| 质粒名称: | mCardinal-N1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 54590 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mCardinal-N1 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | McCardinal（主干上的C终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | mCardinal-N1 was a gift from Michael Davidson (Addgene plasmid # 54590 ; http://n2t.net/addgene:54590 ; RRID:Addgene_54590) |
| 参考文献部分: | Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein. Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia KC, Contag CH, Shen K, Blau HM, Lin MZ. Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888. 10.1038/nmeth.2888 PubMed 24633408 |
买家导航
mCardinalN1载体基本信息
mCardinalN1载体图谱质粒图谱和多克隆位点信息
mCardinalN1载体简介
mCardinalN1载体序列
LOCUS mCardinal-N1 4747 bp ds-DNA circular SYN 10-APR-2019
DEFINITION Localization: N1 Cloning Vector, Excitation: 604, Emission: 659.
ACCESSION .
VERSION .
KEYWORDS mCardinal-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4747)
AUTHORS Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS,
Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia
KC, Contag CH, Shen K, Blau HM, Lin MZ
TITLE Non-invasive intravital imaging of cellular differentiation with a
bright red-excitable fluorescent protein.
JOURNAL Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
PUBMED 24633408
REFERENCE 2 (bases 1 to 4747)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 10, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4747
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 94..397
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 398..601
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 552..572
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 624..704
/label=MCS
/note="multiple cloning site"
regulatory 706..715
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 712..1446
/codon_start=1
/product="far-red fluorescent protein derived from
mNeptune, with further red-shifted spectra"
/label=mCardinal
/translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
ARYCDLPSKLGHKLNGMDELYK"
polyA_signal 1568..1689
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1605..1624)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1659..1678
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1696..2151)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1833..1854)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2045..2064
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2178..2282
/gene="bla"
/label=AmpR promoter
promoter 2284..2641
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2307..2327)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2492..2627
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2554..2573
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2676..3470
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2730..2749)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3340..3359
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3658..3677)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3702..3749
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4078..4666
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4567..4586
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
61 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
121 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
181 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
241 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
301 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
361 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
421 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
481 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
541 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
601 tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt
661 cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc
721 aagggcgagg agctgatcaa ggagaacatg cacatgaagc tgtacatgga aggcaccgtg
781 aacaaccacc acttcaagtg caccaccgaa ggggagggca agccctacga gggcacccag
841 acccagagga ttaaggtggt ggagggaggc cccctgccgt tcgcattcga catcctggcc
901 acctgcttta tgtacgggag caagaccttc atcaaccaca cccagggcat ccccgatttc
961 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
1021 ggcgtgctta ccgttaccca ggacaccagc ctccaggacg gctgcttgat ctacaacgtc
1081 aagctcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
1141 tgggaggcca ccaccgagac cctgtacccc gctgacggcg gcctggaagg cagatgcgac
1201 atggccctga agctcgtggg cgggggccac ctgcactgca acctgaagac cacatacaga
1261 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actttgtgga ccgcagactg
1321 gaaagaatca aggaggccga caatgagacc tacgtcgagc agcacgaggt ggctgtggcc
1381 agatactgcg acctccctag caaactgggg cacaaactta atggcatgga cgagctgtac
1441 aagtaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggttttact
1501 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
1561 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
1621 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
1681 tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
1741 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
1801 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
1861 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
1921 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
1981 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
2041 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
2101 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
2161 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
2221 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
2281 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
2341 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
2401 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2461 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
2521 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
2581 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
2641 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
2701 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
2761 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
2821 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
2881 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
2941 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
3001 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
3061 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
3121 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
3181 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
3241 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
3301 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
3361 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
3421 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
3481 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
3541 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
3601 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
3661 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
3721 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
3781 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
3841 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
3901 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
3961 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
4021 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
4081 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
4141 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
4201 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
4261 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
4321 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
4381 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
4441 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
4501 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
4561 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
4621 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
4681 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
4741 atcccct
//
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