| 质粒名称: | pBA2219 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 38152 |
| 存储实验室: | David Waugh |
|
载体骨架基本信息 |
|
| 载体名称: | p15A |
| 载体出品公司: | Novagen |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 插入p15A复制子的T7启动子盒和网关克隆盒。 |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | DsbC |
| 物种来源: | E. coli |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | 388476123 |
| 启动子 | T7 |
| 标签/融合蛋白: | N-末端先导肽（克隆产物）（主干上的N-末端） |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 promoter |
| 3' 测序引物 | T7 terminator |
|
文献引用方法 |
|
| 材料和方法部分: | pBA2219 was a gift from David Waugh (Addgene plasmid # 38152 ; http://n2t.net/addgene:38152 ; RRID:Addgene_38152) |
| 参考文献部分: | Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3). Austin BP, Waugh DS. Protein Expr Purif. 2012 Mar;82(1):116-24. Epub 2011 Dec 14. 10.1016/j.pep.2011.11.015 PubMed 22197595 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBA2219 3619 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBA2219
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3619)
AUTHORS Austin BP, Waugh DS
TITLE Isolation of Metarhizium anisopliae carboxypeptidase A with native
disulfide bonds from the cytosol of Escherichia coli BL21(DE3).
JOURNAL Protein Expr Purif. 2012 Mar;82(1):116-24. Epub 2011 Dec 14.
PUBMED 22197595
REFERENCE 2 (bases 1 to 3619)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3619
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 108..653
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind 554..573
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 770..787
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(1237..1256)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 1248..2063
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(1321..1340)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 2665..2684
/label=T7
/note="T7 promoter, forward primer"
promoter 2665..2683
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 2715..2737
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 2744..2776
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
protein_bind 2786..2810
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(3460..3484)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind complement(3533..3551)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 3547..3594
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
61 gagttttcgt tccactgagc gtcagacccc ttaataagat gatcttcttg agatcgtttt
121 ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg
181 aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca
241 ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct
301 aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc
361 aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca
421 gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac
481 gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga
541 gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
601 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct
661 ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc
721 gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt
781 gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt
841 ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc
901 agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc
961 aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt
1021 tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt
1081 tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa
1141 gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
1201 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
1261 aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg
1321 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg
1381 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
1441 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
1501 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa
1561 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
1621 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
1681 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
1741 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc
1801 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
1861 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
1921 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
1981 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
2041 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
2101 ttacgctgac ttgacgggac ggcggctttg ttgaataaat cgaacttttg ctgagttgaa
2161 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa
2221 aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg
2281 gctggatgat ggggcgattc aggcctggta tgagtcagca acaccttctt cacgaggcag
2341 acctcagcgc tcaaagatgc aggggtaaaa gctaaccgca tctttaccga caaggcatcc
2401 ggcagttcaa cagatcggga agggctggat ttgctgagga tgaaggtgga ggaaggtgat
2461 gtcattctgg tgaagaagct cgaccgtctt ggccgcgaca ccgccgacat gatccaactg
2521 ataaaagagt ttgatgctca gggtgtagcg gttcggttta ttgacgacgg gatcagtacc
2581 gacggtgata tggggcaaat ggtggtcacc atcctgtcgg ctgtggcaca ggctgaacgc
2641 cggaggatct cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc
2701 cctctagaaa taattttgtt taactttaag aaggagatat accatggcta gcatgactgg
2761 tggacagcaa atgggtcgga tcatcacaag tttgtacaaa aaagttgtgg acgacgcggc
2821 aattcaacaa acgttagcca aaatgggcat caaaagcagc gatattcagc ccgcgcctgt
2881 agctggcatg aagacagttc tgactaacag cggcgtgttg tacatcaccg atgatggtaa
2941 acatatcatt caggggccaa tgtatgacgt tagtggcacg gctccggtca atgtcaccaa
3001 taagatgctg ttaaagcagt tgaatgcgct tgaaaaagag atgatcgttt ataaagcgcc
3061 gcaggaaaaa cacgtcatca ccgtgtttac tgatattacc tgtggttact gccacaaact
3121 gcatgagcaa atggcagact acaacgcgct ggggatcacc gtgcgttatc ttgctttccc
3181 gcgccagggg ctggacagcg atgcagagaa agaaatgaaa gctatctggt gtgcgaaaga
3241 taaaaacaaa gcgtttgatg atgtgatggc aggtaaaagc gtcgcaccag ccagttgcga
3301 cgtggatatt gccgaccatt acgcacttgg cgtccagctt ggcgttagcg gtactccggc
3361 agttgtgctg agcaatggca cacttgttcc gggttaccag ccgccgaaag agatgaaaga
3421 attcctcgac gaacaccaaa aaatgaccag cggtaaataa tgcaactttc ttgtacaaag
3481 tggtgatcgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
3541 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
3601 ggaggaacta tatccggat
//
