| 质粒名称: | pCDH-EF1-Luc2-P2A-tdTomato |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 72486 |
| 存储实验室: | Kazuhiro Oka |
|
载体骨架基本信息 |
|
| 载体名称: | pCDH-CMV-MCS |
| 载体出品公司: | Systembio |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 7133 bp |
| 完整质粒大小: | 10289 bp |
| 载体修饰: | ClaI/SalI fragment was replaced with EF1 + new MCS |
| 载体类型: | 哺乳细胞表达，Lentiviral |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Luc2-P2A-tdTomato |
| 物种来源: | Synthetic |
| 插入大小: | 3174 bp |
| 启动子 | EF1 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | GCACTTGATGTAATTCTCC |
| 3' 测序引物 | CAACACCACGGAATTGTCAG |
|
文献引用方法 |
|
| 材料和方法部分: | pCDH-EF1-Luc2-P2A-tdTomato was a gift from Kazuhiro Oka (爱迪基因 plasmid # 72486) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCDH-EF1-Luc2-P2A-tdTomato 10289 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION Express Luc2 and tdTomato under EF1 promoter.
ACCESSION .
VERSION .
KEYWORDS pCDH-EF1-Luc2-P2A-tdTomato
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10289)
TITLE express gene of interest under truncated EF-1 promoter
JOURNAL Unpublished
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCDH-EF1-Luc2-P2A-tdTomato|
FEATURES Location/Qualifiers
source 1..10289
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 234..414
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 458..583
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1076..1309
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 1804..1921
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2013..3191
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2244..3182
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3139..3159
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 3207..4856
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
CDS 4872..4928
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4938..6368
/codon_start=1
/product="tandem dimeric (pseudo-monomeric) derivative of
DsRed (Shaner et al., 2004)"
/label=tdTomato
/note="mammalian codon-optimized"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
RSEGRHHLFLYGMDELYK"
misc_feature 6384..6972
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6437..6457)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6855..6866)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7046..7279
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7351..7472
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7388..7407)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7442..7461
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 7491..7626
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7553..7572
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(7664..7680)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7664..7680)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7677..7699)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7688..7704
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7712..7742)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7757..7778
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7895..7912)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8066..8654)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8146..8165)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8825..9685)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9448..9467
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9686..9790)
/gene="bla"
/label=AmpR promoter
primer_bind 9858..9876
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(9914..9936)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 10036..10055
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 10249..10271
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 10263..10280
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 10264..10280
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
1921 tatcgatagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
1981 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
2041 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
2101 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
2161 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
2221 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
2281 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
2341 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
2401 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
2461 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
2521 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
2581 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
2641 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
2701 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
2761 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
2821 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
2881 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
2941 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
3001 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
3061 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
3121 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
3181 aggtgtcgtg agcaattgga tccaccatgg aagatgccaa aaacattaag aagggcccag
3241 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc
3301 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta
3361 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc
3421 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg
3481 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc
3541 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag
3601 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca
3661 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt
3721 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca
3781 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc
3841 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca
3901 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca
3961 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc
4021 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat
4081 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga
4141 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc
4201 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca
4261 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta
4321 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg
4381 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca
4441 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact
4501 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag
4561 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc
4621 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa
4681 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc
4741 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg
4801 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgaagc
4861 ttggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc
4921 ctggacctcc cgggccaatg gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct
4981 tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
5041 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
5101 tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga
5161 agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg
5221 agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc
5281 aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc
5341 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg
5401 acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc
5461 tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact
5521 acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt
5581 acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc agcaccggca
5641 gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc aaagagttca
5701 tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
5761 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
5821 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt
5881 acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca
5941 agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct
6001 ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg
6061 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
6121 cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc
6181 actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct
6241 actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
6301 aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg gacgagctgt
6361 acaagtaata agaattcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga
6421 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
6481 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
6541 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
6601 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
6661 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
6721 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
6781 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
6841 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
6901 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
6961 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
7021 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat
7081 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
7141 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
7201 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
7261 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
7321 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
7381 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
7441 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
7501 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
7561 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
7621 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg
7681 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
7741 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
7801 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
7861 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
7921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
7981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
8041 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
8101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
8161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
8221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
8281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
8341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
8401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
8461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
8521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
8581 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
8641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
8701 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
8761 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
8821 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
8881 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
8941 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
9001 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
9061 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
9121 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
9181 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
9241 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
9301 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
9361 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
9421 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
9481 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
9541 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
9601 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
9661 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
9721 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
9781 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
9841 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
9901 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
9961 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
10021 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
10081 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
10141 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
10201 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
10261 gttgtaaaac gacggccagt gccaagctg
//
