| 质粒名称: | pLR-NI |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31006 |
| 存储实验室: | Adam Bogdanove, Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pCS468 |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 2460 bp |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | last NI repeat |
| 物种来源: | Xanthamonas oryzae |
| 插入大小: | 107 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | ApaI(未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | pTC14_F1 (cctactcaggagagcgttca) |
|
文献引用方法 |
|
| 材料和方法部分: | pLR-NI was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 31006 ; http://n2t.net/addgene:31006 ; RRID:Addgene_31006) |
| 参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
pLRNI载体基本信息
pLRNI载体图谱质粒图谱和多克隆位点信息
pLRNI载体简介
pLRNI载体序列
LOCUS pLR-NI 2568 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLR-NI
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2568)
AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller
JA, Somia NV, Bogdanove AJ, Voytas DF
TITLE Efficient design and assembly of custom TALEN and other TAL
effector-based constructs for DNA targeting.
JOURNAL Nucleic Acids Res. 2011 Apr 14. ():.
PUBMED 21493687
REFERENCE 2 (bases 1 to 2568)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2568
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 1..17
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 498..1289
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 1382..1970
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1871..1890
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2124..2141
/label=L4440
/note="L4440 vector, forward primer"
terminator 2300..2327
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 2419..2505
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 2554..8
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2568..17
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
ORIGIN
1 gtaaaacgac ggccagtctt aagctcgggc ccgtctcgct atcgccagca acattggcgg
61 caagcaagcg ctcgaaagca ttgtggccca gctgagccgg cctgatccgg cgttggccgc
121 gttgaccaac gagagacgtc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
181 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
241 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
301 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
361 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
421 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
481 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
541 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
601 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
661 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
721 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
781 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
841 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
901 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
961 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
1021 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
1081 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
1141 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
1201 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
1261 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
1321 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
1381 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
1441 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
1501 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
1561 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
1621 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
1681 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
1741 cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag
1801 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
1861 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
1921 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
1981 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
2041 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
2101 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
2161 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
2221 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
2281 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
2341 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
2401 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
2461 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
2521 ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgtt
//
