| 质粒名称: | pTRKH2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 71312 |
| 存储实验室: | Rodolphe Barrangou, Todd Klaenhammer |
|
载体骨架基本信息 |
|
| 载体名称: | pIL253 |
| 载体出品公司: | -- |
| 载体抗性: | 红霉素 |
| 空载体大小: | 4963 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 插入pSA34，pIC-19R MCS |
| 载体类型: | 细菌表达；大肠杆菌革兰氏阳性菌穿梭载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pTRKH2 was a gift from Rodolphe Barrangou & Todd Klaenhammer (Addgene plasmid # 71312 ; http://n2t.net/addgene:71312 ; RRID:Addgene_71312) |
| 参考文献部分: | High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening. O'Sullivan DJ, Klaenhammer TR. Gene. 1993 Dec 31;137(2):227-31. PubMed 8299952 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTRKH2 6719 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Theta replicating shuttle vector for E. coli and gram positive
bacteria. High copy number in gram positives. Erythromycin
resistance, recommended to use BHI agar plates for E. coli..
ACCESSION .
VERSION .
KEYWORDS pTRKH2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6719)
AUTHORS O'Sullivan DJ, Klaenhammer TR
TITLE High- and low-copy-number Lactococcus shuttle cloning vectors with
features for clone screening.
JOURNAL Gene. 1993 Dec 31;137(2):227-31.
PUBMED 8299952
REFERENCE 2 (bases 1 to 6719)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6719
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 102..123
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 138..168
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 176..192
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 181..203
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 200..216
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 200..216
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 212..454
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITNSSISRSRARESLAAGRRIPGNSLAVVLQRRDWENPGVTQ
LNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
primer_bind complement(295..312)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(295..311)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(304..326)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5424..5443
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(5510..5612)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(6004..6021)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6138..6683)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(6218..6237)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
61 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
121 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
181 agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt catcgatatc
241 tagatctcga gctcgcgaaa gcttggctgc aggtcgacgg atccccggga attcactggc
301 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
361 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
421 ccaacagttg cgcagcctga atggcgaatg ctagaatcga tacgattttg aagtggcaac
481 agataaaaaa aagcagttta aaattgttgc tgaactttta aaacaagcaa atacaatcat
541 tgtcgcaaca gatagcgaca gagaaggcga aaacattgcc tggtcgatca ttcataaagc
601 aaatgccttt tctaaagata aaacgtataa aagactatgg atcaatagtt tagaaaaaga
661 tgtgatccgt agcggttttc aaaatttgca accaggaatg aattactatc ccttttatca
721 agaagcgcaa aagaaaaacg aaatgataca ccaatcagtg caaaaaaaga tataatggga
781 gataagacgg ttcgtgttcg tgctgacttg caccatatca taaaaatcga aacagcaaag
841 aatggcggaa acgtaaaaga agttatggaa ataagactta gaagcaaact taagagtgtg
901 ttgatagtgc agtatcttaa aattttgtat aataggaatt gaagttaaat tagatgctaa
961 aaatttgtaa ttaagaagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt
1021 tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga
1081 taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag
1141 taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt
1201 aaaactgaat actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa
1261 caaacagagg tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa
1321 aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta
1381 caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat
1441 tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt
1501 cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac
1561 gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt
1621 tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt
1681 attgtctatt tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct
1741 tttgtaaatt tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatac
1801 tactgacagc ttccaaggag ctaaagaggt ccctagcgct cttatcatgg ggaagctcgg
1861 atcatatgca agacaaaata aactcgcaac agcacttgga gaaatgggac gaatcgagaa
1921 aaccctcttt acgctggatt acatatctaa taaagccgta aggagacggg ttcaaaaagg
1981 tttaaataaa ggagaagcaa tcaatgcatt agctagaact atattttttg gacaacgtgg
2041 agaatttaga gaacgtgctc tccaagacca gttacaaaga gctagtgcac taaacataat
2101 tattaacgct ataagtgtgt ggaacactgt atatatggaa aaagccgtag aagaattaaa
2161 agcaagagga gaatttagag aagatttaat gccatatgcg tggccgttag gatgggaaca
2221 tatcaatttt cttggagaat acaaatttga aggattacat gacactgggc aaatgaattt
2281 acgtccttta cgtataaaag agccgtttta ttcttaatat aacggctctt tttatagaaa
2341 aaatccttag cgtggttttt ttccgaaatg ctggcggtac cccaagaatt agaaatgagt
2401 agatcaaatt attcacgaat agaatcagga aaatcagatc caaccataaa aacactagaa
2461 caaattgcaa agttaactaa ctcaacgcta gtagtggatt taatcccaaa tgagccaaca
2521 gaaccagagc cagaaacaga atcagaacaa gtaacattgg atttagaaat ggaagaagaa
2581 aaaagcaatg acttcgtgtg aataatgcac gaaatcgttg cttatttttt tttaaaagcg
2641 gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc catgacacat
2701 ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc caaaccaacg
2761 cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta actttgtttg
2821 aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt caagtatcta
2881 agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt gctttttttg
2941 tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac ttatataaaa
3001 aatagtttat tggagataag aaaattagca aatatctata cactagaaac gtttaagaaa
3061 gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt ttcttcggag
3121 ggggaagatt atatatataa gttaatagaa aataacaaaa taatttattc gattagtgga
3181 aaaaaattga cttataaagg aaaaaaatct ttttcaaaac atgcaatatt gaaacagttg
3241 aatgaaaaag caaaccaagt taattaaaca acctatttta taggatttat aggaaaggag
3301 aacagctgaa tgaatatccc ttttgttgta gaaactgtgc ttcatgacgg cttgttaaag
3361 tacaaattta aaaatagtaa aattcgctca atcactacca agccaggtaa aagcaaaggg
3421 gctatttttg cgtatcgctc aaaatcaagc atgattggcg gtcgtggtgt tgttctgact
3481 tccgaggaag cgattcaaga aaatcaagat acatttacac attggacacc caacgtttat
3541 cgttatggaa cgtatgcaga cgaaaaccgt tcatacacga aaggacattc tgaaaacaat
3601 ttaagacaaa tcaatacctt ctttattgat tttgatattc acacggcaaa agaaactatt
3661 tcagcaagcg atattttaac aaccgctatt gatttaggtt ttatgcctac tatgattatc
3721 aaatctgata aaggttatca agcatatttt gttttagaaa cgccagtcta tgtgacttca
3781 aaatcagaat ttaaatctgt caaagcagcc aaaataattt cgcaaaatat ccgagaatat
3841 tttggaaagt ctttgccagt tgatctaacg tgtaatcatt ttggtattgc tcgcatacca
3901 agaacggaca atgtagaatt ttttgatcct aattaccgtt attctttcaa agaatggcaa
3961 gattggtctt tcaaacaaac agataataag ggctttactc gttcaagtct aacggtttta
4021 agcggtacag aaggcaaaaa acaagtagat gaaccctggt ttaatctctt attgcacgaa
4081 acgaaatttt caggagaaaa gggtttaata gggcgtaata acgtcatgtt taccctctct
4141 ttagcctact ttagttcagg ctattcaatc gaaacgtgcg aatataatat gtttgagttt
4201 aataatcgat tagatcaacc cttagaagaa aaagaagtaa tcaaaattgt tagaagtgcc
4261 tattcagaaa actatcaagg ggctaatagg gaatacatta ccattctttg caaagcttgg
4321 gtatcaagtg atttaaccag taaagattta tttgtccgtc aagggtggtt taaattcaag
4381 aaaaaaagaa gcgaacgtca acgtgttcat ttgtcagaat ggaaagaaga tttaatggct
4441 tatattagcg aaaaaagcga tgtatacaag ccttatttag tgacgaccaa aaaagagatt
4501 agagaagtgc taggcattcc tgaacggaca ttagataaat tgctgaaggt actgaaggcg
4561 aatcaggaaa ttttctttaa gattaaacca ggaagaaatg gtggcattca acttgctagt
4621 gttaaatcat tgttgctatc gatcattaaa gtaaaaaaag aagaaaaaga aagctatata
4681 aaggcgctga caaattcttt tgacttagag catacattca ttcaagagac tttaaacaag
4741 ctagcagaac gccctaaaac ggacacacaa ctcgatttgt ttagctatga tacaggctga
4801 aaataaaacc cgcactatgc cattacattt atatctatga tacgtgtttg ttttttcttt
4861 gctgtttagc gaatgattag cagaaatata cagagtaaga ttttaattaa ttattagggg
4921 gagaaggaga gagtagcccg aaaactttta gttggcttgg actgaacgaa gtgagggaaa
4981 ggctactaaa acgtcgaggg gcagtgagag cgaagcgaac acttgatttt ttaattttct
5041 atcttttata ggtcattaga gtatacttat ttgtcctata aactatttag cagcataata
5101 gatttattga ataggtcatt taagttgagc atattagagg aggaaaatct tggagaaata
5161 tttgaagaac ccgattacat ggattggatt agttcttgtg gttacgtggt ttttaactaa
5221 aagtagtgaa tttttgattt ttggtgtgtg tgtcttgttg ttagtatttg ctagtcaaag
5281 tgattaaata gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
5341 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
5401 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
5461 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
5521 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
5581 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc
5641 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
5701 tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt
5761 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
5821 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg
5881 agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg
5941 caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc
6001 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
6061 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
6121 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
6181 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
6241 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
6301 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
6361 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
6421 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
6481 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
6541 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
6601 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
6661 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctag
//
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