| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141386 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 8825 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 370℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-M |
| 物种来源: | SARS-CoV-2 |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: |
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141386 ; http://n2t.net/addgene:141386 ; RRID:Addgene_141386)
For viral preps, please replace (Addgene plasmid # 141386) in the above sentence with: (Addgene viral prep # 141386-LV)
|
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro 9579 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient
expression and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9579)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 9579)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9579
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2338..3519
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2568..3510
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3467..3487
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 3544..3553
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4234..4257
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 4294..4317
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 4329..4902
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4496..4513)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4723..4742
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4935..5534
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4935..4954)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5431..5451
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 5548..6136
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5601..5621)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6019..6030)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6343..6976
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7104..7120)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7104..7120)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7117..7139)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7128..7144
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7152..7182)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7197..7218
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7335..7352)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7506..8094)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7586..7605)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8265..9125)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8888..8907
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9126..9230)
/gene="bla"
/label=AmpR promoter
polyA_signal 9278..9412
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9315..9334)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9369..9388
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
3541 gccgccacca tggccgactc aaatgggacc attactgtag aggagctgaa aaaactgttg
3601 gagcaatgga accttgtgat tggatttctc ttcttgacat ggatttgtct cctgcaattc
3661 gcatacgcga atcgcaaccg attcctctat attataaaac ttatattcct ttggctcctg
3721 tggcctgtga cccttgcctg cttcgtactc gcggcggtgt atcgaataaa ctggataact
3781 ggcggcatag caatagctat ggcatgcctg gtaggtttga tgtggctctc ctattttata
3841 gcgtcattca gactttttgc acgcacgcga agcatgtggt catttaaccc tgaaacaaat
3901 atcttgctta atgtccccct gcatggcaca attttgaccc gaccgctcct tgaatcagaa
3961 ttggtgattg gagccgtgat cctgaggggc cacttgagga tcgcaggaca tcatcttggt
4021 cgctgtgata ttaaggattt gccaaaagaa ataactgtag ctaccagtcg aactttgagc
4081 tattacaagc ttggagcgtc ccaaagggtc gctggcgata gtggatttgc agcctattca
4141 cgctacagga tagggaacta taaactgaat acagaccact catcatcttc tgataacatt
4201 gctttgctcg tccagctcga aggcggcggg ggatggagcc atccacaatt cgagaaaggc
4261 ggtggttcag gaggaggtag cgggggtgga tcatggtcac atccgcagtt tgaaaagtaa
4321 ggatcccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
4381 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
4441 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
4501 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
4561 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
4621 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
4681 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
4741 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
4801 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
4861 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa
4921 ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
4981 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
5041 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
5101 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
5161 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
5221 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
5281 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
5341 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
5401 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
5461 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
5521 agcccggtgc ctagacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
5581 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
5641 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
5701 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
5761 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
5821 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
5881 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
5941 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
6001 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
6061 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
6121 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
6181 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
6241 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
6301 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
6361 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
6421 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
6481 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
6541 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
6601 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
6661 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
6721 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
6781 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
6841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
6901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
6961 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
7021 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct
7081 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
7141 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
7201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
7261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
7321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
7381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
7441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
7501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
7561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
7621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
7681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
7741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
7801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
7861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
7921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
7981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
8041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
8101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
8161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
8221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
8281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
8341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
8401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
8461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
8521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
8581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
8641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
8701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
8761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
8821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
8881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
8941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
9001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
9061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
9121 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
9181 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
9241 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
9301 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
9361 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
9421 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
9481 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
9541 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
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