| 质粒名称: | pMXs-Sox2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 13367 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pMXs |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4600 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SRY-box containing gene 2,Sox2 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 960 bp |
| 基因突变: | None |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pMX-S1811 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMXs-Sox2 was a gift from Shinya Yamanaka (Addgene plasmid # 13367 ; http://n2t.net/addgene:13367 ; RRID:Addgene_13367) |
| 参考文献部分: | Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174 |
买家导航
pMXsSox2载体基本信息
pMXsSox2载体图谱质粒图谱和多克隆位点信息
pMXsSox2载体简介
pMXsSox2载体序列
LOCUS pMXs-Sox2 5766 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Retroviral expression of mouse Sox2.
ACCESSION .
VERSION .
KEYWORDS pMXs-Sox2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5766)
AUTHORS Takahashi K, Yamanaka S
TITLE Induction of pluripotent stem cells from mouse embryonic and adult
fibroblast cultures by defined factors.
JOURNAL Cell. 2006 Aug 25. 126(4):663-76.
PUBMED 16904174
REFERENCE 2 (bases 1 to 5766)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5766
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(2..21)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(674..1534)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1297..1316
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1535..1639)
/gene="bla"
/label=AmpR promoter
LTR 1669..2262
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 2325..2682
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 2741..3157
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 3087..3109
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 3125..3141
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 3167..3541
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind 3479..3495
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
protein_bind 3592..3616
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(4577..4601)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
LTR 4918..5387
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(5517..5534)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5688..510)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
61 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
601 atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa
661 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
721 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
781 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
841 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
901 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
961 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
1021 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
1081 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
1141 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
1201 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
1261 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
1321 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
1381 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
1441 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
1501 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
1561 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
1621 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcataa tgaaagaccc
1681 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
1741 ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
1801 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
1861 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
1921 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
1981 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
2041 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
2101 acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
2161 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
2221 tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat
2281 cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gccagcaact
2341 tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct gcgtcggtac
2401 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttcggaaca
2461 cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg tggcccgacc
2521 tgagtccaaa aatcccgatc gttttggact ctttggtgca cccccctaat aggagggata
2581 tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct
2641 ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg ttctgtgttg
2701 tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg ttaccactcc
2761 cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt
2821 agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt
2881 cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt
2941 cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc
3001 cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc
3061 tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc
3121 ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat atgagatctt
3181 atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa gagttactaa
3241 cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg aagtctggag
3301 acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc acccttaccg
3361 agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac ctcgctggaa
3421 aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg gcatcgcagc
3481 ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat cctctagact
3541 gccggatcta gctagttaat taaggatccc agtgtggtgg tacgggaaat cacaagtttg
3601 tacaaaaaag caggcttgta taacatgatg gagacggagc tgaagccgcc gggcccgcag
3661 caagcttcgg ggggcggcgg cggaggaggc aacgccacgg cggcggcgac cggcggcaac
3721 cagaagaaca gcccggaccg cgtcaagagg cccatgaacg ccttcatggt atggtcccgg
3781 gggcagcggc gtaagatggc ccaggagaac cccaagatgc acaactcgga gatcagcaag
3841 cgcctgggcg cggagtggaa acttttgtcc gagaccgaga agcggccgtt catcgacgag
3901 gccaagcggc tgcgcgctct gcacatgaag gagcacccgg attataaata ccggccgcgg
3961 cggaaaacca agacgctcat gaagaaggat aagtacacgc ttcccggagg cttgctggcc
4021 cccggcggga acagcatggc gagcggggtt ggggtgggcg ccggcctggg tgcgggcgtg
4081 aaccagcgca tggacagcta cgcgcacatg aacggctgga gcaacggcag ctacagcatg
4141 atgcaggagc agctgggcta cccgcagcac ccgggcctca acgctcacgg cgcggcacag
4201 atgcaaccga tgcaccgcta cgacgtcagc gccctgcagt acaactccat gaccagctcg
4261 cagacctaca tgaacggctc gcccacctac agcatgtcct actcgcagca gggcaccccc
4321 ggtatggcgc tgggctccat gggctctgtg gtcaagtccg aggccagctc cagccccccc
4381 gtggttacct cttcctccca ctccagggcg ccctgccagg ccggggacct ccgggacatg
4441 atcagcatgt acctccccgg cgccgaggtg ccggagcccg ctgcgcccag tagactgcac
4501 atggcccagc actaccagag cggcccggtg cccggcacgg ccattaacgg cacactgccc
4561 ctgtcgcaca tgtgaaaccc agctttcttg tacaaagtgg tgatattcca gctgagcgcc
4621 ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg caggccatgt
4681 ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt taaactcgag cggccgccag
4741 cacagtggtc gacgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
4801 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
4861 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
4921 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
4981 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
5041 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
5101 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
5161 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
5221 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
5281 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
5341 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta
5401 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa
5461 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
5521 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
5581 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
5641 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
5701 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
5761 ggacta
//
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