| 质粒名称: | pBAM1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 60487 |
| 存储实验室: | Víctor de Lorenzo |
|
载体骨架基本信息 |
|
| 载体名称: | pBAM1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素和卡那霉素 |
| 空载体大小: | 4384 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 合成生物学 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBAM1 was a gift from Víctor de Lorenzo (Addgene plasmid # 60487 ; http://n2t.net/addgene:60487 ; RRID:Addgene_60487) |
| 参考文献部分: | pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes. Martinez-Garcia E, Calles B, Arevalo-Rodriguez M, de Lorenzo V. BMC Microbiol. 2011 Feb 22;11:38. doi: 10.1186/1471-2180-11-38. 10.1186/1471-2180-11-38 PubMed 21342504 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBAM1 4384 bp ds-DNA circular SYN 20-MAR-2020
DEFINITION This plasmids is a modular mini-Tn5 vector that could be used to
generate random mutagenesis libraries or to insert heterologous DNA
into a target genome .
ACCESSION .
VERSION .
KEYWORDS pBAM1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4384)
AUTHORS Martinez-Garcia E, Calles B, Arevalo-Rodriguez M, de Lorenzo V
TITLE pBAM1: an all-synthetic genetic tool for analysis and construction
of complex bacterial phenotypes.
JOURNAL BMC Microbiol. 2011 Feb 22;11:38. doi: 10.1186/1471-2180-11-38.
PUBMED 21342504
REFERENCE 2 (bases 1 to 4384)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 20, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4384
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 4..392
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
oriT 411..519
/note="incP origin of transfer"
terminator 666..705
/label=fd terminator
/note="central terminator from bacteriophage fd (Otsuka and
Kunisawa, 1982)"
CDS complement(709..1569)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1570..1661)
/gene="bla"
/label=AmpR promoter
CDS complement(1718..3145)
/codon_start=1
/product="transposase from the bacterial Tn5 transposon
(Reznikoff, 1993)"
/label=Tn5 transposase
/translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
KLDGFLAAKDLMAQGIKI"
misc_feature 3239..3257
/label=Tn5 ME
/note="hyperactive mosaic end for Tn5 transposase
recognition (Reznikoff et al., 2004)"
primer_bind complement(3354..3373)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 3365..4180
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
misc_feature 4267..4323
/label=MCS
/note="pUC18/19 multiple cloning site"
misc_feature 4355..4373
/label=Tn5 ME
/note="hyperactive mosaic end for Tn5 transposase
recognition (Reznikoff et al., 2004)"
ORIGIN
1 ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg ctttgagagg ctctaagggc
61 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaggctttaa
121 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg
181 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct
241 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg
301 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac
361 tatcaacagg ttgaactgct gatcttcaga tcggcgcgcc ggccggccta cggccagcct
421 cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa
481 cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac
541 caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga
601 tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaaggacaag
661 ggtcgtccaa aaaaaaaggc tccaaaagga gcctttaatt gtatcggttt accaatgttt
721 aatcaggctc gcgccaattt ccgcaatctg acggttacgt tcatccatcg tcgcctggct
781 gccggtggta taaatcacca caatacggct cggtttacca tccgggccca gcgccgcaat
841 aatgccacgg ctgccacgtt cgcccgcacc gcttttatcc gcaataaacc agccagccgg
901 cagcgcgcta cgcagcagcg gacccgccac tttatccgct tccatccaat caatcagttg
961 ctggcggctt gccagggtca gcagctcacc cgtcagcagt ttacgcaggg tggtcgccat
1021 tgctgccggc atggtggtat cacgttcatc gttcggaatc gcttcgttca gttccggttc
1081 ccaacgatcc agacgggtca catgatcgcc catgttatgc agaaacgcgg tcagttcttt
1141 cggaccgcca atggtcgtca gcagcaggtt cgccgcggtg ttatcgctca tggtaatcgc
1201 cgcgctgcac agttcacgca cggtcatgcc atcggtcaga tgtttttcgg tcaccgggct
1261 atattccacc agatcgttct ggctataatg aatacgacgg cccagttgtt cctggcccgc
1321 atccacacgg ctcagaaccg caccgcacag cagcacttta aaggtgctca tcatcggaaa
1381 acgttcttcc ggacgaaaag attccagaat tttgccgctg ttcagatcca gttcaatata
1441 gcccacgcgc gcacccagtt gatcttccgc atctttcact ttcaccaggg tttccggatg
1501 cgcaaacacc ggcaggcaaa acgccgcaaa aaacggaatc agcgccacac gaaaatgctg
1561 aatgctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
1621 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggaat taaaaaaaag
1681 cccgctcatt aggcgggcta ctaatttaaa tcgattagat tttaatgccc tgcgccatca
1741 ggtctttcgc ggccagaaag ccatccagtt tgctttgcag cgcttcccaa ccttcccaca
1801 gcgcacccca gctcgcaatg ccggtacgtt tgctatccat aaagccgccc agacgcgcaa
1861 tcgccatata cgcccattgc aggctgcccg ctttttcttt gcgtttgcgt ttgcctttat
1921 ccagatagcc cagcagttgg cattcatccg gggtcagcac ggtttccgcg ctctggcttt
1981 caacgtgttc cgcttctttc agcaggccct gcgcacgcag tgcttgcggc ggagtaaaag
2041 attcacgcag ttgcagcaga cgcaccgcca caaagctcag aatgctcacc atacgttcca
2101 ggttatccgg ttcttccata cgctgacgtt ccgcacccgc acccgttttc cacgctttgt
2161 gaaattcttc aatgcgccaa cgatgggtat aaatatcaat cacacgcagc gcttgggcca
2221 gactttccac cggctcgctg gtcagcagca gccatttcag cggggtttcg cctttcggcg
2281 gattaatttc ttcggccagc accgcgttca gggtaatgtt gccctgtttc agggtaatac
2341 ggccgctacg caggctcagg ctcgctttac gcgccggacg gtttttacgt ttgccacgtt
2401 tatccaccac gcctttctgc ggaatgctaa tctgatagcc gcccagttcc ggctggtttt
2461 tcaggtgatc atacagatac aggccgcttt ccacatcttt acgcggatgt ttgctacgca
2521 ccacaaaacg ttcgttatgg gccagtttat cttgcagata cgcatgaata tccgcttcac
2581 gatcgcacac cgcaatcacg ttgctcatca tgctgcccat tctcagacgc gaagttgcag
2641 cagcggccag ccatttgccg ctttcttttt catccgcatc cgccggatca tccggacgca
2701 tccaccattc ttgatgcagc aggcccacgg tacgaaaggt ggtcgcttcc agcagcagca
2761 cgctatgcac ccaccaacca cggcttttat cctgaatgct acccagtttg cccagttctt
2821 ccgccacctg atgacgatag ctcagagagg tggtatcttc aattgccagc agttccggaa
2881 attcctgggc cagtttcacg gtctgcatgg cacccgcttt acgaatcgct tccgcgctca
2941 cgttcggatt acgaataaaa cgatacgcgc cttcctgcat ggctttgctg ccttcgctgc
3001 taatggtaat gcttttgccg ctatatttgg ccagttgcgc cgcaacattc accagacgcg
3061 cggtacgacg cggatcaccc agcgcagcac tagaaaacac gcttttcgcc caatccgccg
3121 cacgatgcag tgcactggta atcatgaacg ttaccatgtt aggaggtcac atggaacatc
3181 agattctgga aaacgggaaa ggttccgttc aggacgctac ttgtgtagtt taaaccagct
3241 gtctcttata cacatctgac gtcttgtgtc tcaaaatctc tgatgttaca ttgcacaaga
3301 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
3361 tgttatgagc catattcagc gtgaaacgag ctgtagccgt ccgcgtctga acagcaacat
3421 ggatgcggat ctgtatggct ataaatgggc gcgtgataac gtgggtcaga gcggcgcgac
3481 catttatcgt ctgtatggca aaccggatgc gccggaactg tttctgaaac atggcaaagg
3541 cagcgtggcg aacgatgtga ccgatgaaat ggtgcgtctg aactggctga ccgaatttat
3601 gccgctgccg accattaaac attttattcg caccccggat gatgcgtggc tgctgaccac
3661 cgcgattccg ggcaaaaccg cgtttcaggt gctggaagaa tatccggata gcggcgaaaa
3721 cattgtggat gcgctggccg tgtttctgcg tcgtctgcat agcattccgg tgtgcaactg
3781 cccgtttaac agcgatcgtg tgtttcgtct ggcccaggcg cagagccgta tgaacaacgg
3841 cctggtggat gcgagcgatt ttgatgatga acgtaacggc tggccggtgg aacaggtgtg
3901 gaaagaaatg cataaactgc tgccgtttag cccggatagc gtggtgaccc acggcgattt
3961 tagcctggat aacctgattt tcgatgaagg caaactgatt ggctgcattg atgtgggccg
4021 tgtgggcatt gcggatcgtt atcaggatct ggccattctg tggaactgcc tgggcgaatt
4081 tagcccgagc ctgcaaaaac gtctgtttca gaaatatggc attgataatc cggatatgaa
4141 caaactgcaa tttcatctga tgctggatga atttttctaa taattaattg gggaccctag
4201 aggtcccctt ttttatttta aaaatttttt cacaaaacgg tttacaagca taactagtgc
4261 ggccgcaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
4321 ttcgcgcggc cgcggcctag gcggccttaa ttaaagatgt gtataagaga cagctgggcg
4381 cgcc
//
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