| 质粒名称: | PA-mCherry1-C1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 54495 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | PA-mCherry1-C1 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | PA-mCherry1（主干上的N终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | PA-mCherry1-C1 was a gift from Michael Davidson (Addgene plasmid # 54495 ; http://n2t.net/addgene:54495 ; RRID:Addgene_54495) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS PA-mCherry1-C1 4716 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission:
515 / 517.
ACCESSION .
VERSION .
KEYWORDS PA-mCherry1-C1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4716)
TITLE Michael Davidson Empty Backbones
REFERENCE 2 (bases 1 to 4716)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4716
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 115..418
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 419..622
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 573..593
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 655..664
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 661..1368
/codon_start=1
/product="photoactivatable monomeric derivative of DsRed
fluorescent protein (Subach et al., 2009)"
/label=PAmCherry1
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(806..824)
/label=mCherry-R
/note="mCherry, reverse primer"
misc_feature 1369..1434
/label=MCS
/note="multiple cloning site"
polyA_signal 1558..1679
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1595..1614)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1649..1668
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1686..2141)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1823..1844)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2035..2054
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2168..2272
/gene="bla"
/label=AmpR promoter
promoter 2274..2631
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2297..2317)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2482..2617
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2544..2563
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2666..3460
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2720..2739)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3330..3349
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3648..3667)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3692..3739
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4068..4656
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4557..4576
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc ggtcgccacc
661 atggtgagca agggcgagga ggataacatg gccatcatta aggagttcat gcgcttcaag
721 gtgcacatgg aggggtccgt gaacggccac gtgttcgaga tcgagggcga gggcgagggc
781 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
841 ttcacctggg acatcctgag ccctcagttc atgtacggct ccaatgccta cgtgaagcac
901 cccgccgaca tccccgacta ctttaagctg tccttccccg agggcttcaa gtgggagcgc
961 gtgatgaaat tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
1021 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgtg
1081 atgcagaaga agaccatggg ctgggaggcc ctctccgagc ggatgtaccc cgaggacggc
1141 gccctgaagg gcgaggtcaa gcccagagtg aagctgaagg acggcggcca ctacgacgct
1201 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
1261 aaccgcaagc tggacatcac cagccacaac gaggactaca ccatcgtgga gcagtacgag
1321 agagccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
1381 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga
1441 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
1501 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
1561 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
1621 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
1681 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
1741 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
1801 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
1861 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
1921 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
1981 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
2041 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
2101 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
2161 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
2221 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
2281 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
2341 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
2401 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
2461 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
2521 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
2581 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
2641 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
2701 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
2761 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
2821 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
2881 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
2941 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
3001 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
3061 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
3121 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
3181 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
3241 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
3301 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
3361 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
3421 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
3481 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
3541 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
3601 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
3661 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
3721 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
3781 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
3841 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
3901 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
3961 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
4021 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
4081 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
4141 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
4201 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
4261 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
4321 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
4381 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
4441 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
4501 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
4561 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
4621 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
4681 cggttcctgg ccttttgctg gccttttgct cacatg
//
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