| 质粒名称: | pHAGE_puro |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 118692 |
| 存储实验室: | Christopher Vakoc |
|
载体骨架基本信息 |
|
| 载体名称: | pHAGE_puro |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7576 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHAGE_puro was a gift from Christopher Vakoc (Addgene plasmid # 118692 ; http://n2t.net/addgene:118692 ; RRID:Addgene_118692) |
| 参考文献部分: | A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence. Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G, Mackay JP, Kinney JB, Shi J, Vakoc CR. Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29. 10.1016/j.ccell.2018.10.015 PubMed 30503706 |
买家导航
pHAGEpuro载体基本信息
pHAGEpuro载体图谱质粒图谱和多克隆位点信息
pHAGEpuro载体简介
pHAGEpuro载体序列
LOCUS pHAGE_puro 7585 bp ds-DNA circular SYN 07-DEC-2018
DEFINITION Empty vector to express overexpression cDNA.
ACCESSION .
VERSION .
KEYWORDS pHAGE_puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7585)
AUTHORS Christopher Vakoc
TITLE A transcription factor addiction in leukemia imposed by the MLL
promoter sequence
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7585)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7585
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 276..295
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 364..963
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(364..383)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 860..880
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 977..1565
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(1030..1050)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(1448..1459)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 1640..1873
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(1992..2010)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 2078..2182
/gene="bla"
/label=AmpR promoter
CDS 2183..3043
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2401..2420)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3214..3802
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3703..3722
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3956..3973
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 4065..4084
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 4213..4234
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4249..4279
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4287..4303
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4292..4314
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 4311..4327
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4311..4327
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 4377..4396
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 4588..5221
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 5268..5393
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5890..6123
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 6645..6762
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 6814..7117
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 7118..7321
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 7272..7292
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 7318..7342
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 7429..343
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(7464..7487)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
ORIGIN
1 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
61 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
121 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
181 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
241 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
301 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
361 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
421 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
481 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
541 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
601 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
661 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
721 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
781 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
841 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
901 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
961 tgaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
1021 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
1081 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
1141 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
1201 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
1261 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
1321 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
1381 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
1441 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
1501 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
1561 cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
1621 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
1681 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
1741 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
1801 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
1861 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
1921 aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg
1981 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
2041 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
2101 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
2161 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
2221 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
2281 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
2341 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
2401 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
2461 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
2521 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
2581 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
2641 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
2701 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
2761 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
2821 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
2881 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
2941 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
3001 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
3061 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
3121 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
3181 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
3241 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
3301 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
3361 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
3421 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
3481 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
3541 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
3601 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
3661 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
3721 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
3781 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
3841 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
3901 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
3961 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
4021 gccgattcat taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc
4081 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
4141 ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga
4201 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
4261 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
4321 ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
4381 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
4441 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
4501 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
4561 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
4621 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
4681 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
4741 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
4801 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
4861 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
4921 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
4981 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
5041 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
5101 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
5161 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
5221 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
5281 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
5341 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
5401 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
5461 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
5521 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
5581 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
5641 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
5701 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
5761 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
5821 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
5881 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
5941 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
6001 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
6061 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
6121 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
6181 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
6241 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
6301 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
6361 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
6421 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
6481 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
6541 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
6601 tcgacggtat cgccgaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg
6661 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
6721 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
6781 agcagagatc cagtttggac tagtggagtt ccgcgttaca taacttacgg taaatggccc
6841 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
6901 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
6961 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
7021 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
7081 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
7141 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
7201 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
7261 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
7321 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
7381 aagacaccgg cggccgcacc ggtctgcagc tagctcgaga attctaccgg gtaggggagg
7441 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
7501 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
7561 cgttctttgg tggccccttc gcgcc
//
