| 质粒名称: | pT7-NSP2SA11 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 89169 |
| 存储实验室: | Takeshi Kobayashi |
|
载体骨架基本信息 |
|
| 载体名称: | p3E5 |
| 空载体大小: | 3074 bp |
| 完整质粒大小: | 4133 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Rotavirus SA11 NSP2 |
| 物种来源: | Rotavirus SA11 |
| 插入大小: | 1059 bp |
| GenBank编号 | LC178571 |
| 启动子: | T7 promotor |
| 克隆方法: | Gibson克隆 |
| 5’测序引物 | T7 forward CTGTGGATAACCGTATTACCG |
| 3’测序引物 | T7 terminal GCTAGTTATTGCTCAGCGG |
|
文献引用方法 |
|
| 材料和方法部分: | pT7-NSP2SA11 was a gift from Takeshi Kobayashi (Addgene plasmid # 89169 ; http://n2t.net/addgene:89169 ; RRID:Addgene_89169) |
| 参考文献部分: | Entirely plasmid-based reverse genetics system for rotaviruses. Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M, Matsuura Y, Taniguchi K, Kobayashi T. Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi: 10.1073/pnas.1618424114. 10.1073/pnas.1618424114 PubMed 28137864 |
买家导航
pT7NSP2SA11载体基本信息
pT7NSP2SA11载体图谱质粒图谱和多克隆位点信息
pT7NSP2SA11载体简介
pT7NSP2SA11载体序列
LOCUS pT7-NSP2SA11 4133 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Express Rotavirus SA11 NSP2 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pT7-NSP2SA11
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4133)
AUTHORS Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N, Onishi M,
Matsuura Y, Taniguchi K, Kobayashi T
TITLE Entirely plasmid-based reverse genetics system for rotaviruses.
JOURNAL Proc Natl Acad Sci U S A. 2017 Jan 30. pii: 201618424. doi:
10.1073/pnas.1618424114.
PUBMED 28137864
REFERENCE 2 (bases 1 to 4133)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4133
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(641..1501)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1264..1283
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1502..1606)
/gene="bla"
/label=AmpR promoter
primer_bind 1674..1692
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(1730..1752)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 1852..1871
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2081..2100
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 2390..2408
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 2390..2407
/label=SP6
/note="SP6 promoter, forward primer"
terminator 2476..2523
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind 2519..2537
/label=T7 Term
/note="T7 terminator, reverse primer"
promoter complement(3741..3759)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3844..3861)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4015..470)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4095..4114)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
61 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
121 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
181 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
241 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
301 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
361 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
421 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
481 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
541 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
601 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
661 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
721 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
781 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
841 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
901 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
961 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1021 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1081 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1141 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1201 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1261 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1321 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1381 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1441 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1501 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1561 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1621 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
1681 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
1741 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
1801 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
1861 agcagattgt actgagagtg caccattcga cgctctccct tatgcgactc ctgcattaag
1921 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcaagg
1981 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
2041 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
2101 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
2161 tctggctagc gatgaccctg ctgattggtt cgctgaccat ttccgggtgc gggacggcgt
2221 taccagaaac tcagaaggtt cgtccaacca aaccgactct gacggcagtt tacgagagag
2281 atgatagggt ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt
2341 tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac
2401 actatagaat acaagcttgc atgcctgcag gtcgactcta gaggatctcg atccggatat
2461 agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
2521 tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc
2581 cggatccttt tttttgagct ctcccttagc catccgagtg gacgacgtcc tccttcggat
2641 gcccaggtcg gaccgcgagg aggtggagat gccatgccga cccggtcaca taagcgcttt
2701 ctattcttgc taagccatca tcatcctcaa attgatagcg aattaaacgc caacttgaga
2761 aacttcgtcc atttttctat ccgttgacag ccctttaaat ggattttttt ctggtttcat
2821 tttttggaaa agcaaccttt tacacacgtc aagtgtatta ccctgtttca ttgatgatgt
2881 aaatgcatac cagttttgcc aaataattct ttgatcaagt aggttaaaat cattattaac
2941 tccagtttta acattactct tgaaagttgc atatactctg tcagcgtgat tagcaactga
3001 tgaatacttt acaattctat aatgaccctt accatgtgtg attacagcaa acttgttata
3061 ttgccatcga agttcagcga ctaattcttt aacatgaaca tctgaaattg gtttatcttc
3121 gttcaatgta actttatatt caataaaatt ctgatccaga attggcatca attgatgttc
3181 taaataagtt agtttccaca tggtgaaggc agcgttttga aatacaattt ctcctccttc
3241 tgcagttatt gtagtttcaa tttctttaga ctgtccaata gcaatcagtg ttgatttcaa
3301 aagtaaatct tttgaatgaa atagaatatc ctgtggattt tctttacgta tcaccaaatt
3361 ttctaaatgc cttattgata ctactcgtga taatacatta gagacgtttg cttgcgtcac
3421 tttcaatgaa tttagagctt cacatatcaa catagccacc ttagtaaaca taattgtttc
3481 aaaattcatg cctcttgaat tatcattagt attataccgt ttcttaaatt gaggaggcgg
3541 tgctattcca taaataattg aatcataaaa cttatccatg tcctttttgt ctactttagc
3601 tgtcagcata gctttaatag ctaaattatt aaaaggaata aatttatagc tatcattctc
3661 caaatgagga tagcaaaagc aagctagctc agccatggct acaccgcaag gctcaaacgg
3721 cgactgagac gctttaaaag cctatagtga gtcgtattaa cccgggatcg atatccccct
3781 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
3841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
3901 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
3961 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
4021 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
4081 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgc
//
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