| 质粒名称: | pLV-hTERT-IRES-hygro |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 85140 |
| 存储实验室: | Tobias Meyer |
|
载体骨架基本信息 |
|
| 载体名称: | pLV-EF1a-IRES-Hygro |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 9211 bp |
| 完整质粒大小: | 12578 bp |
| 载体类型: | 慢病毒表达 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | hTERT,TERT |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3399 bp |
| 启动子 | EF1a |
| 克隆方法: | Gibson克隆 |
| 5' 测序引物 | TCAAGCCTCAGACAGTGGTTC |
| 3' 测序引物 | ACACCGGCCTTATTCCAA |
|
文献引用方法 |
|
| 材料和方法部分: | pLV-hTERT-IRES-hygro was a gift from Tobias Meyer (Addgene plasmid # 85140 ; http://n2t.net/addgene:85140 ; RRID:Addgene_85140) |
| 参考文献部分: | Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells. Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, Betzig E, Meyer T. Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub 2016 Nov 14. 10.1038/ncb3438 PubMed 27842057 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLV-hTERT-IRES-hygro 12531 bp ds-DNA circular SYN 16-APR-2019
DEFINITION Lentiviral expression of hTERT, used to create immortalized cell
lines.
ACCESSION .
VERSION .
KEYWORDS pLV-hTERT-IRES-hygro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12531)
AUTHORS Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A,
Betzig E, Meyer T
TITLE Engulfed cadherin fingers are polarized junctional structures
between collectively migrating endothelial cells.
JOURNAL Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub
2016 Nov 14.
PUBMED 27842057
REFERENCE 2 (bases 1 to 12531)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 16, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12531
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2221..2432
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
intron 2433..3371
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3328..3348
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
primer_bind complement(7043..7060)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 7270..7289
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 7451..8476
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
misc_feature 8500..9088
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8553..8573)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8971..8982)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 9295..9928
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(10056..10072)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10056..10072)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10069..10091)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10080..10096
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10104..10134)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10149..10170
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10287..10304)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10458..11046)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10538..10557)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11217..12077)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11840..11859
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12078..12182)
/gene="bla"
/label=AmpR promoter
polyA_signal 12230..12364
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(12267..12286)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 12321..12340
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
3361 ttccatttca ggtgtcgtga ggatccatgc cgcgcgctcc ccgctgccga gccgtgcgct
3421 ccctgctgcg cagccactac cgcgaggtgc tgccgctggc cacgttcgtg cggcgcctgg
3481 ggccccaggg ctggcggctg gtgcagcgcg gggacccggc ggctttccgc gcgctggtgg
3541 cccagtgcct ggtgtgcgtg ccctgggacg cacggccgcc ccccgccgcc ccctccttcc
3601 gccaggtgtc ctgcctgaag gagctggtgg cccgagtgct gcagaggctg tgcgagcgcg
3661 gcgcgaagaa cgtgctggcc ttcggcttcg cgctgctgga cggggcccgc gggggccccc
3721 ccgaggcctt caccaccagc gtgcgcagct acctgcccaa cacggtgacc gacgcactgc
3781 gggggagcgg ggcgtggggg ctgctgctgc gccgcgtggg cgacgacgtg ctggttcacc
3841 tgctggcacg ctgcgcgctc tttgtgctgg tggctcccag ctgcgcctac caggtgtgcg
3901 ggccgccgct gtaccagctc ggcgctgcca ctcaggcccg gcccccgcca cacgctagtg
3961 gaccccgaag gcgtctggga tgcgaacggg cctggaacca tagcgtcagg gaggccgggg
4021 tccccctggg cctgccagcc ccgggtgcga ggaggcgcgg gggcagtgcc agccgaagtc
4081 tgccgttgcc caagaggccc aggcgtggcg ctgcccctga gccggagcgg acgcccgttg
4141 ggcaggggtc ctgggcccac ccgggcagga cgcgtggacc gagtgaccgt ggtttctgtg
4201 tggtgtcacc tgccagaccc gccgaagaag ccacctcttt ggagggtgcg ctctctggca
4261 cgcgccactc ccacccatcc gtgggccgcc agcaccacgc gggcccccca tccacatcgc
4321 ggccaccacg tccctgggac acgccttgtc ccccggtgta cgccgagacc aagcacttcc
4381 tctactcctc aggcgacaag gagcagctgc ggccctcctt cctactcagc tctctgaggc
4441 ccagcctgac tggcgctcgg aggctcgtgg agaccatctt tctgggttcc aggccctgga
4501 tgccagggac tccccgcagg ttgccccgcc tgccccagcg ctactggcaa atgcggcccc
4561 tgtttctgga gctgcttggg aaccacgcgc agtgccccta cggggtgctc ctcaagacgc
4621 actgcccgct gcgagctgcg gtcaccccag cagccggtgt ctgtgcccgg gagaagcccc
4681 agggctctgt ggcggccccc gaggaggagg acacagaccc ccgtcgcctg gtgcagctgc
4741 tccgccagca cagcagcccc tggcaggtgt acggcttcgt gcgggcctgc ctgcgccggc
4801 tggtgccccc aggcctctgg ggctccaggc acaacgaacg ccgcttcctc aggaacacca
4861 agaagttcat ctccctgggg aagcatgcca agctctcgct gcaggagctg acgtggaaga
4921 tgagcgtgcg gggctgcgct tggctgcgca ggagcccagg ggttggctgt gttccggccg
4981 cagagcaccg tctgcgtgag gagatcctgg ccaagttcct gcactggctg atgagtgtgt
5041 acgtcgtcga gctgctcagg tctttctttt atgtcacgga gaccacgttt caaaagaaca
5101 ggctcttttt ctaccggaag agtgtctgga gcaagttgca aagcattgga atcagacagc
5161 acttgaagag ggtgcagctg cgggagctgt cggaagcaga ggtcaggcag catcgggaag
5221 ccaggcccgc cctgctgacg tccagactcc gcttcatccc caagcctgac gggctgcggc
5281 cgattgtgaa catggactac gtcgtgggag ccagaacgtt ccgcagagaa aagagggccg
5341 agcgtctcac ctcgagggtg aaggcactgt tcagcgtgct caactacgag cgggcgcggc
5401 gccccggcct cctgggcgcc tctgtgctgg gcctggacga tatccacagg gcctggcgca
5461 ccttcgtgct gcgtgtgcgg gcccaggacc cgccgcctga gctgtacttt gtcaaggtgg
5521 atgtgacggg cgcgtacgac accatccccc aggacaggct cacggaggtc atcgccagca
5581 tcatcaaacc ccagaacacg tactgcgtgc gtcggtatgc cgtggtccag aaggccgccc
5641 atgggcacgt ccgcaaggcc ttcaagagcc acgtctctac cttgacagac ctccagccgt
5701 acatgcgaca gttcgtggct cacctgcagg agaccagccc gctgagggat gccgtcgtca
5761 tcgagcagag ctcctccctg aatgaggcca gcagtggcct cttcgacgtc ttcctacgct
5821 tcatgtgcca ccacgccgtg cgcatcaggg gcaagtccta cgtccagtgc caggggatcc
5881 cgcagggctc catcctctcc acgctgctct gcagcctgtg ctacggcgac atggagaaca
5941 agctgtttgc ggggattcgg cgggacgggc tgctcctgcg tttggtggat gatttcttgt
6001 tggtgacacc tcacctcacc cacgcgaaaa ccttcctcag gaccctggtc cgaggtgtcc
6061 ctgagtatgg ctgcgtggtg aacttgcgga agacagtggt gaacttccct gtagaagacg
6121 aggccctggg tggcacggct tttgttcaga tgccggccca cggcctattc ccctggtgcg
6181 gcctgctgct ggatacccgg accctggagg tgcagagcga ctactccagc tatgcccgga
6241 cctccatcag agccagtctc accttcaacc gcggcttcaa ggctgggagg aacatgcgtc
6301 gcaaactctt tggggtcttg cggctgaagt gtcacagcct gtttctggat ttgcaggtga
6361 acagcctcca gacggtgtgc accaacatct acaagatcct cctgctgcag gcgtacaggt
6421 ttcacgcatg tgtgctgcag ctcccatttc atcagcaagt ttggaagaac cccacatttt
6481 tcctgcgcgt catctctgac acggcctccc tctgctactc catcctgaaa gccaagaacg
6541 cagggatgtc gctgggggcc aagggcgccg ccggccctct gccctccgag gccgtgcagt
6601 ggctgtgcca ccaagcattc ctgctcaagc tgactcgaca ccgtgtcacc tacgtgccac
6661 tcctggggtc actcaggaca gcccagacgc agctgagtcg gaagctcccg gggacgacgc
6721 tgactgccct ggaggccgca gccaacccgg cactgccctc agacttcaag accatcctgg
6781 actgagaatt cctcgagggc ggccgctcta gagtcgacgg gccgcggtaa caattgttaa
6841 ctaacttaag ctagcaacgg tttccctcta gcgggatcaa ttccgccccc cccccctaac
6901 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
6961 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
7021 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
7081 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
7141 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
7201 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
7261 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
7321 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
7381 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
7441 cgataatacc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga
7501 aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt
7561 cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt
7621 ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt
7681 gcttgacatt ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg
7741 tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga
7801 ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg
7861 accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc
7921 ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc
7981 tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc
8041 ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg
8101 gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc
8161 gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc
8221 aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag
8281 cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt
8341 ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg
8401 gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc
8461 gagggcaaag gaatagcccg ggcggggcgc gtctggaaca atcaacctct ggattacaaa
8521 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
8581 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
8641 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
8701 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
8761 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
8821 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
8881 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
8941 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
9001 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
9061 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
9121 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
9181 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
9241 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
9301 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
9361 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
9421 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
9481 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
9541 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
9601 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
9661 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
9721 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
9781 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
9841 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
9901 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
9961 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta
10021 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
10081 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
10141 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
10201 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
10261 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
10321 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
10381 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
10441 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
10501 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
10561 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
10621 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
10681 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
10741 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
10801 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
10861 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
10921 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
10981 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
11041 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
11101 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
11161 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
11221 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
11281 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
11341 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
11401 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
11461 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
11521 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
11581 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
11641 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
11701 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
11761 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
11821 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
11881 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
11941 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
12001 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
12061 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
12121 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
12181 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
12241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
12301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
12361 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
12421 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
12481 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
