| 出品人: | Fodor,B.D.(2004) |
|---|---|
| 载体名称: | pMHE5Tc |
| 质粒类型: | 广宿主载体；细菌表达载体;体外表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T7/lac |
| 载体大小: | 6032 bp |
| 5' 测序引物及序列: | T7 promoter 5’TAATACGACTCACTATAGGG 3’ |
| 3' 测序引物及序列: | T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’ |
| 载体标签: | 6XHis tag（N-ter）；FLAG-tag、Strep-tag II (C-ter) |
| 载体抗性: | 四环素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pMHE5Tc载体质粒基本信息
pMHE5Tc质粒图谱载体图谱和pMHE5Tc载体序列质粒序列多克隆位点信息
pMHE5Tc质粒载体简介
pMHE5Tc质粒序列载体序列
LOCUS AY299697 6032 bp DNA circular SYN 10-FEB-2004
DEFINITION Broad host range expression vector pMHE5Tc, complete sequence.
ACCESSION AY299697
VERSION AY299697.1 GI:34334148
TITLE Modular broad-host-range expression vectors for single-protein and
protein complex purification
JOURNAL Appl. Environ. Microbiol. 70 (2), 712-721 (2004)
PUBMED 14766546
AUTHORS Fodor,B.D.
JOURNAL Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of
Sciences, Temesvari krt., Szeged, Csongrad 6701, Hungary
FEATURES Location/Qualifiers
source 1..6032
/organism="Broad host range expression vector pMHE5Tc"
/mol_type="genomic DNA"
/db_xref="taxon:244055"
primer_bind 187..205
/note="T7 promoter primer"
regulatory 187..203
/regulatory_class="promoter"
/note="T7 promoter"
misc_feature 204..229
/note="lac operator"
regulatory 260..266
/regulatory_class="ribosome_binding_site"
misc_feature 274..276
/note="fusion protein start codon"
misc_feature 280..297
/note="His-tag"
misc_feature 298..309
/note="Factor Xa cleavage site"
misc_feature 364..387
/note="FLAG-tag"
misc_feature 373..387
/note="enterokinase cleavage site"
misc_feature 391..414
/note="Strep-tag II"
primer_bind complement(468..485)
/note="T7 terminator primer"
regulatory 481..528
/regulatory_class="terminator"
/note="T7 terminator"
rep_origin 1160..1822
/note="pBBR1 oriV"
misc_feature 2816..3809
/note="RP4 mob; oriT"
regulatory complement(3899..4014)
/regulatory_class="terminator"
/note="T4 terminator"
gene complement(4320..5510)
/gene="tet"
CDS complement(4320..5510)
/gene="tet"
/codon_start=1
/transl_table=11
/product="tetracycline resistance protein"
/protein_id="AAQ64668.1"
/db_xref="GI:34334149"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
regulatory 5895..6010
/regulatory_class="terminator"
/note="T4 terminator"
ORIGIN
1 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
61 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
121 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
181 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
241 taattttgtt taactttaag aaggagatat accatggggc atcatcatca tcatcatatc
301 gagggaaggc ctgtcgacgg tatcgataag cttgatatcg aattcctgca gctcgaggga
361 tccgactaca aggacgacga cgacaagaac tggagccatc cgcagttcga gaagtgaaga
421 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
481 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
541 aactatatcc ggatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt
601 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
661 ccaataggcc gactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
721 gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga
781 aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt
841 atgtctattg ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct
901 caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga
961 tcatttattc tgcctcccag agcctgataa aaacggtgaa tccgttagcg aggtgccgcc
1021 ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca
1081 gacaaggtat agggcggcga ggcggctaca gccgatagtc tggaacagcg cacttacggg
1141 ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg
1201 gctcgccatc gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc
1261 gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg
1321 gctggctggg cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg
1381 tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa
1441 ccaccacggc ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca
1501 cgcggtcatt gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga
1561 tccagcgctc ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga
1621 tgagcttgga aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca
1681 cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct
1741 catgcgcttt gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga
1801 tttctctgga ctgcgtggcc atgcttatct ccatgcggta gggtgccgca cggttgcggc
1861 accatgcgca atcagctgca acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa
1921 gtggcagtca attacagatt ttctttaacc tacgcaatga gctattgcgg ggggtgccgc
1981 aatgagctgt tgcgtacccc ccttttttaa gttgttgatt tttaagtctt tcgcatttcg
2041 ccctatatct agttctttgg tgcccaaaga agggcacccc tgcggggttc ccccacgcct
2101 tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc
2161 ggccttcggc cttgcccaag gtggcgctgc ccccttggaa cccccgcact cgccgccgtg
2221 aggctcgggg ggcaggcggg cgggcttcgc cttcgactgc ccccactcgc ataggcttgg
2281 gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg ctgcgcgagc cttgacccgc
2341 cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc cgcaggccgg aggcttttcc
2401 ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg ccgcgcagtt ggagccggtg
2461 ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct gtggtcaagc tcgtgggcag
2521 gcgcagcctg tccatcagct tgtccagcag ggttgtccac gggccgagcg aagcgagcca
2581 gccggtggcc gctcgcggcc atcgtccaca tatccacggg ctggcaaggg agcgcagcga
2641 ccgcgcaggg cgaagcccgg agagcaagcc cgtagggcgc cgcagccgcc gtaggcggtc
2701 acgactttgc gaagcaaagt ctagtgagta tactcaagca ttgagtggcc cgccggaggc
2761 accgccttgc gctgcccccg tcgagccggt tggacaccaa aagggagggg caggcatggc
2821 ggcatacgcg atcatgcgat gcaagaagct ggcgaaaatg ggcaacgtgg cggccagtct
2881 caagcacgcc taccgcgagc gcgagacgcc caacgctgac gccagcagga cgccagagaa
2941 cgagcactgg gcggccagca gcaccgatga agcgatgggc cgactgcgcg agttgctgcc
3001 agagaagcgg cgcaaggacg ctgtgttggc ggtcgagtac gtcatgacgg ccagcccgga
3061 atggtggaag tcggccagcc aagaacagca ggcggcgttc ttcgagaagg cgcacaagtg
3121 gctggcggac aagtacgggg cggatcgcat cgtgacggcc agcatccacc gtgacgaaac
3181 cagcccgcac atgaccgcgt tcgtggtgcc gctgacgcag gacggcaggc tgtcggccaa
3241 ggagttcatc ggcaacaaag cgcagatgac ccgcgaccag accacgtttg cggccgctgt
3301 ggccgatcta gggctgcaac ggggcatcga gggcagcaag gcacgtcaca cgcgcattca
3361 ggcgttctac gaggccctgg agcggccacc agtgggccac gtcaccatca gcccgcaagc
3421 ggtcgagcca cgcgcctatg caccgcaggg attggccgaa aagctgggaa tctcaaagcg
3481 cgttgagacg ccggaagccg tggccgaccg gctgacaaaa gcggttcggc aggggtatga
3541 gcctgcccta caggccgccg caggagcgcg tgagatgcgc aagaaggccg atcaagccca
3601 agagacggcc cgagaccttc gggagcgcct gaagcccgtt ctggacgccc tggggccgtt
3661 gaatcgggat atgcaggcca aggccgccgc gatcatcaag gccgtgggcg aaaagctgct
3721 gacggaacag cgggaagtcc agcgccagaa acaggcccag cgccagcagg aacgcgggcg
3781 cgcacatttc cccgaaaagt gccacctggc ggcgttgtga caatttaccg aacaactccg
3841 cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatag
3901 ctttatgctt gtaaaccgtt ttgtgaaaaa atttttaaaa taaaaaaggg gacctctagg
3961 gtccccaatt aattagtaat ataatctatt aaaggtcatt caaaaggtca tccaccggat
4021 caattcccct gctcgcgcag gctgggtgcc aagctctcgg gtaacatcaa ggcccgatcc
4081 ttggagccct tgccctcccg cacgatgatc gtgccgtgat cgaaatccag atccttgacc
4141 cgcagttgca aaccctcact gatccgccga gatgcgccgc gtgcggctgc tggagatggc
4201 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
4261 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt
4321 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
4381 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc
4441 gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct
4501 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
4561 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
4621 gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc
4681 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
4741 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
4801 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
4861 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
4921 aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag
4981 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
5041 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
5101 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
5161 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
5221 cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg
5281 agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga
5341 aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa
5401 tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
5461 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
5521 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagctcgcg cagatcagtt
5581 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
5641 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
5701 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
5761 cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg
5821 ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta
5881 ctaagctgat ccggtggatg accttttgaa tgacctttaa tagattatat tactaattaa
5941 ttggggaccc tagaggtccc cttttttatt ttaaaaattt tttcacaaaa cggtttacaa
6001 gcataaagct caaggagatg gcgcccaaca gt
//
