| 产品名称： | Gibco Advanced DMEM/F-12培养基、Gibco Advanced DMEM/F-12高糖培养基 |
|---|---|
| 产品又名： | Gibco Advanced DMEM/F-12细胞培养基、Gibco Advanced DMEM/F-12细胞培养基 |
| 英文名称： | Gibco Advanced DMEM/F-12 |
| 培养基类型： | 哺乳细胞培养基 |
| 级别： | For Mammalian Cell Culture |
| 品牌： | Gibco |
| 产品目录号： | 12634010，12634-010 |
| 葡萄糖: | 高糖 |
| 谷氨酰胺： | 不含L-谷氨酰胺 |
| 酚红： | 含酚红 |
| 丙酮酸钠： | 含丙酮酸钠 |
| HEPES | 不含HEPES |
| 环保特征: | 可循环使用包装 |
| 产品规格： | 500ml |
| 血清添加: | 使用时，正常浓度添加血清。 |
| 运输条件： | 室温 |
| 产品外观： | 红色液体 |
| 颜色与澄清度： | 红色透明至微不透明液体。 |
| 保存条件： | 密封，2-8°C保存。 |
| 注意事项： | 避免呼入和皮肤接触。 |
| Gibco： |
新鲜酵母浸出液 热灭活马血清 |
Gibco Advanced DMEM/F-12培养基图片
Gibco Advanced DMEM/F-12细胞培养基产品描述：
Advanced DMEM/F-12（Dulbecco改良的Eagle培养基/Ham的F-12）是一种广泛使用的基础培养基，可用于哺乳动物细胞的培养。与传统的DMEM/F-12相比，血清补充剂可以减少50-90%，而生长速度和形态没有变化。在晚期DMEM/F-12中成功培养的细胞无适应性，包括MRC-5、SP2、Vero、WI-38和Jurkat。Gibco Advanced DMEM/F-12培养基使用方法
高级DMEM/F-12与其他培养基不同，因为添加了以下成分以减少血清：乙醇胺、谷胱甘肽、抗坏血酸、胰岛素、转铁蛋白、AlbuMAX®II细胞培养用富含脂质的牛血清白蛋白，以及微量元素亚硒酸钠、偏钒酸铵、硫酸铜，氯化锰。晚期DMEM/F-12需要补充1-5%胎牛血清和4 mM L-谷氨酰胺或谷氨酰胺™ 补充。许多细胞系不需要适应这种介质。必须为每个细胞系优化FBS浓度，以获得最大的血清还原率。先进的DMEM/F-12使用碳酸氢钠缓冲系统（3.7 g/L），因此需要5–10%的二氧化碳环境来维持生理pH值。cGMP生产与质量体系
为了供应链的连续性，我们在纽约州格兰德岛和英国苏格兰的两个独立工厂生产先进的DMEM/F-12。这两个工厂都符合cGMP生产要求，通过了ISO 13485认证，并在FDA注册为医疗器械制造商。Gibco Advanced DMEM/F-12培养基配方
| Components | Molecular Weight | Concentration (mg/L) | mM |
| Amino Acids | |||
| Glycine | 75 | 18.75 | 0.25 |
| L-Alanine | 89 | 4.45 | 0.049999997 |
| L-Arginine hydrochloride | 211 | 147.5 | 0.69905216 |
| L-Asparagine-H2O | 150 | 7.5 | 0.05 |
| L-Aspartic acid | 133 | 6.65 | 0.05 |
| L-Cysteine hydrochloride-H2O | 176 | 17.56 | 0.09977272 |
| L-Cystine 2HCl | 313 | 31.29 | 0.09996805 |
| L-Glutamic Acid | 147 | 7.35 | 0.05 |
| L-Histidine hydrochloride-H2O | 210 | 31.48 | 0.14990476 |
| L-Isoleucine | 131 | 54.47 | 0.41580153 |
| L-Leucine | 131 | 59.05 | 0.45076334 |
| L-Lysine hydrochloride | 183 | 91.25 | 0.4986339 |
| L-Methionine | 149 | 17.24 | 0.11570469 |
| L-Phenylalanine | 165 | 35.48 | 0.2150303 |
| L-Proline | 115 | 17.25 | 0.15 |
| L-Serine | 105 | 26.25 | 0.25 |
| L-Threonine | 119 | 53.45 | 0.44915968 |
| L-Tryptophan | 204 | 9.02 | 0.04421569 |
| L-Tyrosine disodium salt dihydrate | 261 | 55.79 | 0.21375479 |
| L-Valine | 117 | 52.85 | 0.4517094 |
| Vitamins | |||
| Ascorbic Acid phosphate | 289.54 | 2.5 | 0.008634386 |
| Biotin | 244 | 0.0035 | 1.43E-05 |
| Choline chloride | 140 | 8.98 | 0.06414285 |
| D-Calcium pantothenate | 477 | 2.24 | 0.004696017 |
| Folic Acid | 441 | 2.65 | 0.006009071 |
| Niacinamide | 122 | 2.02 | 0.016557377 |
| Pyridoxine hydrochloride | 206 | 2 | 0.009708738 |
| Riboflavin | 376 | 0.219 | 5.82E-04 |
| Thiamine hydrochloride | 337 | 2.17 | 0.006439169 |
| Vitamin B12 | 1355 | 0.68 | 5.02E-04 |
| i-Inositol | 180 | 12.6 | 0.07 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111 | 116.6 | 1.0504504 |
| Cupric sulfate (CuSO4-5H2O) | 250 | 0.0013 | 5.20E-06 |
| Ferric Nitrate (Fe(NO3)3"9H2O) | 404 | 0.05 | 1.24E-04 |
| Ferric sulfate (FeSO4-7H2O) | 278 | 0.417 | 0.0015 |
| Magnesium Chloride (anhydrous) | 95 | 28.64 | 0.30147368 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120 | 48.84 | 0.407 |
| Potassium Chloride (KCl) | 75 | 311.8 | 4.1573334 |
| Sodium Bicarbonate (NaHCO3) | 84 | 2438 | 29.02381 |
| Sodium Chloride (NaCl) | 58 | 6995.5 | 120.61207 |
| Sodium Phosphate dibasic (Na2HPO4) anhydrous | 142 | 71.02 | 0.50014085 |
| Sodium Phosphate monobasic (NaH2PO4-H2O) | 138 | 62.5 | 0.45289856 |
| Zinc sulfate (ZnSO4-7H2O) | 288 | 0.864 | 0.003 |
| Proteins | |||
| AlbuMAX® II | 400 | Infinity | |
| Human Transferrin (Holo) | 7.5 | Infinity | |
| Insulin Recombinant Full Chain | 10 | Infinity | |
| Reducing Agents | |||
| Glutathione, monosodium | 307 | 1 | 0.003257329 |
| Trace Elements | |||
| Ammonium Metavanadate | 116.98 | 3.00E-04 | 2.56E-06 |
| Manganous Chloride | 198 | 5.00E-05 | 2.53E-07 |
| Sodium Selenite | 173 | 0.005 | 2.89E-05 |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 3151 | 17.505556 |
| Ethanolamine | 97.54 | 1.9 | 0.019479187 |
| Hypoxanthine Na | 159 | 2.39 | 0.015031448 |
| Linoleic Acid | 280 | 0.042 | 1.50E-04 |
| Lipoic Acid | 206 | 0.105 | 5.10E-04 |
| Phenol Red | 376.4 | 8.1 | 0.021519661 |
| Putrescine 2HCl | 161 | 0.081 | 5.03E-04 |
| Sodium Pyruvate | 110 | 110 | 1 |
| Thymidine | 242 | 0.365 | 0.001508265 |
Gibco细胞培养基选择指南
| Transfection Reagent （细胞转染试剂） | ||
|---|---|---|
| 产品描述 | 规格 | 货号 |
|
Lipofectamin 2000 （Lipo2000、Lipo 2000） Each unit contains one vial of Lipofectamine 2000 Reagent. |
0.75 ml |
11668-027 11668027 |
|
Lipofectamin 3000 （Lipo3000、lipo 3000） Contains one vial of Lipofectamine 3000 Reagent and one vial of P3000TM Enhancer Reagent. |
0.75 ml |
L3000-008 L3000008 |
| Dulbecco’s Modified Eagle Media （DMEM培养基） | ||
| 产品描述 | 规格 | 货号 |
|
DMEM (1X), liquid (high glucose) Contains no L-glutamine or sodium pyruvate |
500 mL |
11960-044 11960044 |
|
DMEM (1X), liquid (high glucose)
Contains L-glutamine, but no sodium pyruvate
|
500 mL |
11965-092 11965092 |
|
DMEM (1X), liquid (high glucose) (DMEM高糖培养基，最常用)
Contains L-glutamine and sodium pyruvate
|
500 mL |
11995-065 11995065 |
|
DMEM (1X), liquid (high glucose)
Contains 110mg/L sodium pyruvate, but no L-glutamine
|
500 mL |
10313-021 10313021 |
|
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red
|
500 mL |
21063-029 21063029 |
|
DMEM (1X), liquid (high glucose)
Contains no L-glutamine, sodium pyruvate, or phenol red
|
500 mL |
31053-028 31053028 |
|
DMEM (1X), liquid (low glucose)
Contains L-glutamine and sodium pyruvate.
|
500 mL |
11885-084 11885084 |
|
DMEM (1X), liquid (high glucose)
Contains L-glutamine, and 25 mM HEPES, but no sodium pyruvate
|
500 mL |
12430-054 12430054 |
|
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, 15 mM HEPES butter, but no phenol red
|
500 mL |
11039-021 11039021 |
|
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, but no HEPES buffer
|
500 mL |
11320-033 11320033 |
|
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine and 15 mM HEPES buffer
|
500 mL |
11330-032 11330032 |
| RPMI Media （RPMI1640培养基） | ||
| 产品描述 | 规格 | 货号 |
|
RPMI 1640 (1X), liquid（最常用）
Contains L-glutamine
|
500 mL |
11875-093 11875093 |
|
RPMI 1640 (1X), liquid
Contains L-glutamine, but no phenol red
|
500 mL |
11835-030 11835030 |
|
RPMI 1640 (1X), liquid
Contains no L-glutamine
|
500 mL |
21870-076 21870076 |
|
RPMI 1640 (1X), liquid
Contains L-glutamine and 25 mM HEPES buffer
|
500 mL |
22400-089 22400089 |
| Minimum Essential Media （MEM培养基） | ||
| 产品描述 | 规格 | 货号 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts and L-glutamine
|
500 mL |
11095-080 11095080 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts and non-essential amino acids, but no L-glutamine
|
500 mL |
10370-021 10370021 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts, but no L-glutamine
|
500 mL |
11090-081 11090081 |
|
MEM α (1X), liquid
Contains L-glutamine, ribonucleosides, and deoxyribonuclosides
|
500 mL |
12571-063 12571063 |
|
MEM α Medium (1X), liquid
Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides
|
500 mL |
12561-056 12561056 |
|
MEM α Medium (1X), liquid
Contains L-glutamine, ribonucleosides and deoxyribonucleosides,
but no phenol red
|
500 mL |
41061-029 41061029 |
| GlutaMAX™ Media （GlutaMAX系列培养基） | ||
| 产品描述 | 规格 | 货号 |
|
DMEM (1X), liquid (high glucose)
Contains 110 mg/L sodium pyruvate
|
500 mL |
10569-010 10569010 |
|
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate
|
500 mL |
10564-011 10564011 |
|
DMEM (1X), liquid (high glucose)
Contains no sodium pyruvate
|
500 mL |
10566-016 10566016 |
|
DMEM (1X), liquid (low glucose)
Contains 110 mg/L sodium pyruvate
|
500 mL |
10567-014 10567014 |
|
DMEM/F12 (1X), liquid 1:1
Contains no HEPES buffer
|
500 mL |
10565-018 10565018 |
| F12 Nutrient Mixture (1X), liquid | 500 mL |
31765-035 31765035 |
|
IMDM (1X), liquid
Contains 25 mM HEPES buffer and 3,024 mg/L sodium bicarbonate
|
500 mL |
31980-030 31980-030 |
|
MEM α (1X), liquid （最常用）
Contains no ribonucleosides or deoxyribonucleosides
|
500 mL |
32561-037 32561037 |
|
MEM α (1X), liquid
Contains ribonucleosides and deoxyribonucleosides
|
500 mL |
32571-036 32571036 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts
|
500 mL |
41090-036 41090036 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts and 25 mM HEPES buffer
|
500 mL |
42360-032 42360032 |
| OptiMEM® I Reduced Serum Medium (1X), liquid | 500 mL |
51985-034 51985034 |
| RPMI 1640 (1X), liquid | 500 mL |
61870-036 61870036 |
|
RPMI 1640 (1x), liquid
Contains 25 mM HEPES buffer
|
500 mL |
72400-047 72400047 |
| 其他常用基础培养基 | ||
| 产品描述 | 规格 | 货号 |
|
F12 Nutrient Mixture (1X), liquid
Contains L-glutamine
|
500 mL |
11765-054 11765054 |
|
F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid
Contains L-glutamine
|
500 mL |
21127-022 21127022 |
|
IMDM (1X), liquid
Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol
|
12440-053 12440053 |
|
|
Liebovitz’s L-15 Medium (1X), liquid (L15 Medium)
Contains L-glutamine
|
11415-064 11415064 |
|
|
McCoy’s 5A Medium (modified) (1X), liquid
Contains L-glutamine
|
16600-082 16600082 |
|
|
Medium 199 (1X), liquid
Contains Earle’s salts, L-glutamine, and 2,200 mg/L sodium bicarbonate
|
11150-059 11150059 |
|
| Reduced Serum Media 细胞减血清培养基 | ||
| 产品描述 | 规格 | 货号 |
| Advanced D-MEM (1X), liquid | 500 mL |
12491-015 12491015 |
| Advanced D-MEM/F12 (1X), liquid | 500 mL |
12634-010 12634010 |
| Advanced MEM (1X), liquid | 500 mL |
12492-013 12492013 |
| Advanced RPMI 1640 (1X), liquid | 500 mL |
12633-012 12633012 |
| Opti-MEM®-I Reduced Serum Medium (1X), liquid | 500 mL |
31985-070 31985070 |
| Balanced Salt Solutions 细胞培养缓冲液 | ||
| 产品描述 | 规格 | 货号 |
|
DPBS (1X), liquid
Contains calcium and magnesium
|
500 mL |
14040-133 14040133 |
|
DPBS (1X), liquid
Contains no calcium or magnesium
|
500 mL |
14190-144 14190144 |
|
DPBS (10X), liquid
Contains no calcium or magnesium
|
500 mL |
14200-075 14200075 |
|
HBSS (1X), liquid
Contains calcium and magnesium, but no phenol red
|
500 mL |
14025-092 14025092 |
|
HBSS (1X), liquid
Contains no calcium or magnesium
|
500 mL |
14170-112 14170112 |
|
HBSS (1X), liquid
Contains no calcium chloride, magnesium chloride,
magnesium sulfate, or phenol red
|
500 mL |
14175-095 14175-095 |
| PBS 7.4 (1X), liquid | 500 mL |
10010-023 10010023 |
| PBS 7.4 (10X), liquid | 500 mL |
70011-044 70011044 |
| PBS 7.2 (1X), liquid | 500 mL |
20012-027 20012027 |
| PBS 7.2 (10X), liquid | 500 mL |
70013-032 70013032 |
| Insect Media 昆虫培养基 | ||
| 产品描述 | 规格 | 货号 |
|
Grace’s Insect Medium, Supplemented (1X), liquid
Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, and L-glutamine, but no insect hemolymph
|
500 mL |
11605-094 11605094 |
|
Schneider’s Drosophila Medium (1X), liquid
Contains L-glutamine
|
500 mL |
11720-034 11720034 |
|
SF900-II SFM (1X), liquid
Contains L-Glutamine, Insect cell culture tested
|
1000 mL |
10902-088 10902088 |
| Reagents 细胞培养试剂 | ||
| 产品描述 | 规格 | 货号 |
|
TrypLE™ Express Dissociation Enzyme with Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
|
100 mL |
12605-010 12605010 |
|
TrypLE™ Express Dissociation Enzyme without Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
|
100 mL |
12604-013 12604013 |
|
Trypsin-EDTA (0.25% Trypsin, EDTA•4Na) (1X), liquid（胰蛋白酶，最常用）
Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
|
100 mL |
25200-056 25200056 |
|
Trypsin-EDTA (0.05% Trypsin, EDTA•4Na) (1X), liquid
Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red |
100 mL |
25300-054 25300054 |
|
HEPES Buffer Solution (1M)
Contains 238.3 g/L prepared in distilled water
|
100 mL |
15630-080 15630080 |
| L-glutamine-200 mM (100X), liquid | 100 mL |
25030-081 25030081 |
| GlutaMAX™-I (100X), liquid | 100 mL |
35050-061 35050061 |
|
Antibiotic-Antimycotic (100X), liquid
10,000 units penicillin (base), 10,000 units streptomycin (base), and 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Fungizone™ Antimycotic in 0.85% saline
|
100 mL |
15240-062 15240062 |
|
Penicillin-Streptomycin, liquid （青链霉素双抗）
10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline
|
100 mL |
15140-122 15140122 |
| MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid | 100 mL |
11140-050 11140050 |
|
Distilled Water（无菌水）
Membrane filtered and endotoxin screened
|
500 mL |
15230-162 15230162 |
