| 保藏机构: | NBRCC |
|---|---|
| 菌种名称: | NBRC102228 , NBRC 102228 |
| 英文名称: | Azorhizophilus paspali |
| 中文名称: | 帕斯帕利固氮菌 |
| 菌种又名: | Azotobacter paspali |
| 存储日期: |
2006/12/08
|
| 历史来源: | IAM 12667 <- ATCC 23833 <- D.B. Johnstone <- J. Dobereiner, AX-8A |
| 其他保藏中心编号: | ATCC 23833=DSM 2283=IAM 12667=JCM 20726=LMG 3864=NCAIM B.01794=NCIB 12095=NRRL B-14628 |
| 菌种代号: | AX-8A=WR-129 |
| 培养基: |
965
AZOTOBACTER MEDIUM
CaCl2 x 2 H2O 0.10 g
MgSO4 x 7 H2O 0.10 g
Na2MoO4 x 2 H2O 5.00 mg
K2HPO4 0.90 g
KH2PO4 0.10 g
FeSO4 x 7 H2O 0.01 g
CaCO3 5.00 g
Agar 15.00 g
Distilled water 950.00 ml
Glucose 5.00 g
Mannitol 5.00 g
Adjust pH to 7.3. Sterilize glucose and mannitol separately (in 50 ml H2O) and add to the medium after autoclaving. Calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies.
|
| 培养温度: | 30°C |
| 是否需氧: | 是 |
| 分离来源: | |
| 生物安全等级: | 1 |
| 菌株类型: | 是 |
| 参考文献: |
B1418
1981. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 6. Int. J. Syst. Bacteriol. 31: 215-218.
B2120
Malik, K. A. 1988. A new freeze-drying method for the preservation of nitrogen-fixing and other fragile bacteria. J. Microbiol. Methods 8:259-271.
B2410
Dobereiner, J. 1966. Azotobacter paspali, sp. nov., una bacteria fixadora de nitragenio na rizosferade Paspalum. Pesquisa Agropecuaria Brasileira. 1: 357-365.
B2411
Thompson, J. P., Skerman, V. B. D. 1979. Azotobacteraceae - the taxonomy and ecology of the aerobic nitrogen-fixing bacteria.Academic Press.
B3485
Skerman, V. B. D., McGowan, V., Sneath, P. H. A. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420.
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