| 产品名称： | PMSY培养基；ATCC培养基 2657 |
|---|---|
| 英文名称： | PMSY Medium；ATCC medium 2657 |
| 培养基类型： | 加富培养基 |
| 级别： | for microbiology |
| 品牌： | ELITE-MEDIA（艾礼培养基） |
| 产品目录号： | M2372 |
| 产品规格： | 250g、500g |
| 产品外观： | 乳白色至浅黄色粉末。 |
| 灭菌后颜色与澄清度： | 浅琥珀色，透明，无沉淀。 |
| 保存条件： | 密封，2-25°C保存。 |
| 注意事项： | 避免摄入、吸入、皮肤接触。 |
| 相关产品： | -- |
产品描述：
胰蛋白胨大豆肉汤（TSB）培养基是加富培养基，用于营养需求高的微生物（难养菌）的培养和回收，不需要加血清。胰蛋白胨大豆肉汤培养基适用范围广泛，可用于培养好氧菌、厌氧菌和真菌。例如：
Bacillus subtilis 枯草芽孢杆菌
Bacteroides vulgatus 普通拟杆菌
Candida albicans 白色念珠菌
Escherichia coli 大肠杆菌
Micrococcus luteus 藤黄微球菌
Staphylococcus aureus 金黄色葡萄球菌
Staphylococcus epidermidis 表皮葡萄球菌
Streptococcus pneumonia 肺炎链球菌
Streptococcus pyogenes 酿脓链球菌
用途：
作为致病菌的富集培养基。用于配制血液培养基和血琼脂培养基。
用于无菌试验，检测真菌和好氧菌污染。
在医学上，常用来培养从临床样品中分离的致病微生物。
用于药敏试验和纸片扩散法试验。
配方与配制方法
| 成分 | g/L |
| Na2HPO4 (0.5M) | 25.00 ml |
| NaHPO4 (0.5M) | 25.00 ml |
| (NH4)SO4 (10%) | 10.00 ml |
| Concentrated Base (See Below) | 1.00 ml |
| Na-Thiosulfate (0.1M) | 1.00 ml |
| Yeast extract | 3.00 g |
| Distilled water | 928.00 ml |
121ºC的高压釜15分钟。冷却后，加入10.0 ml过滤灭菌的1M储备溶液琥珀酸钠（2.7g/10ml）。
Concentrated base: per liter
Nitriltriacetic acid (NTA –free acid),...... 20.0 g
MgSO4 anhydrous,............................. 28.9 g
CaCl2.2H2O,................................... 6.7 g
(NH4)6Mo7O24.4H2O...............................18.5 mg
FeSO4.7H2O....................................198.0 g
Hutner’s Metal; 44 (see below)...............100.0 ml
Dissolve NTA separately in 600 ml water and neutralize with KOH (14.6 g KOH) add other components and dissolve in order given. Adjust to pH 6.8 before making to a final volume of 1 liter. A precipitate forms when adjusting the pH from the acid side of 6.8 with KOH (need about 100 ml of 1M KOH ), but eventually will redissolve with stirring. When the pH is near 6.8, the color of the solution changes from a deep yellow to straw color. The final base may be kept for at least a year. Store at 4 oC with a layer of toluene on top to prevent contamination.
Concentrated base: per liter
Nitriltriacetic acid (NTA –free acid),...... 20.0 g
MgSO4 anhydrous,............................. 28.9 g
CaCl2.2H2O,................................... 6.7 g
(NH4)6Mo7O24.4H2O...............................18.5 mg
FeSO4.7H2O....................................198.0 g
Hutner’s Metal; 44 (see below)...............100.0 ml
Dissolve NTA separately in 600 ml water and neutralize with KOH (14.6 g KOH) add other components and dissolve in order given. Adjust to pH 6.8 before making to a final volume of 1 liter. A precipitate forms when adjusting the pH from the acid side of 6.8 with KOH (need about 100 ml of 1M KOH ), but eventually will redissolve with stirring. When the pH is near 6.8, the color of the solution changes from a deep yellow to straw color. The final base may be kept for at least a year. Store at 4 oC with a layer of toluene on top to prevent contamination.
Hutner’s Metal 44: Dissolve the following in order. Do not add components until the previous one has completely dissolved.
Distilled water.............................................800.00 ml
EDTA (free acid, not sodium salt, warm to dissolve)......... 2.50 g
ZnSO4 7H20................................................... 10.95 g
FeSO4.7 H20................................................... 5.00 g
MnSO4. H20.................................................... 1.54 g
CuSO4.5 H20................................................... 0.39 g
Co(NO3)2.6 H20.................................................0.25 g
Na2B4O7.10 H20................................................. 0.18 g
Add a few drops of conc. H2S04 to retard precipitation. Make to a final volume of 1 liter, it should be a clear, lime green solution. Protect from light by wrapping the bottle with Aluminum Foil. Store
