Axenic Dimastigella培养基

产品名称：	Axenic Dimastigella培养基；ATCC培养基 1865
英文名称：	Axenic Dimastigella medium；ATCC medium 1865
培养基类型：	加富培养基
级别：	for microbiology
品牌：	ELITE-MEDIA（艾礼培养基）
产品目录号：	M2746
产品规格：	250g、500g
产品外观：	乳白色至浅黄色粉末。
灭菌后颜色与澄清度：	浅琥珀色，透明，无沉淀。
保存条件：	密封，2-25°C保存。
注意事项：	避免摄入、吸入、皮肤接触。
相关产品：	--

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产品描述：

胰蛋白胨大豆肉汤（TSB）培养基是加富培养基，用于营养需求高的微生物（难养菌）的培养和回收，不需要加血清。胰蛋白胨大豆肉汤培养基适用范围广泛，可用于培养好氧菌、厌氧菌和真菌。

例如：
Bacillus subtilis 枯草芽孢杆菌
Bacteroides vulgatus 普通拟杆菌
Candida albicans 白色念珠菌
Escherichia coli 大肠杆菌
Micrococcus luteus 藤黄微球菌
Staphylococcus aureus 金黄色葡萄球菌
Staphylococcus epidermidis 表皮葡萄球菌
Streptococcus pneumonia 肺炎链球菌
Streptococcus pyogenes 酿脓链球菌

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用途：

作为致病菌的富集培养基。
用于配制血液培养基和血琼脂培养基。
用于无菌试验，检测真菌和好氧菌污染。
在医学上，常用来培养从临床样品中分离的致病微生物。
用于药敏试验和纸片扩散法试验。

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配方与配制方法

成分	g/L
Sonneborn's Paramecium Medium (ATCC Medium 802, see below)	980.0 ml
Vitamin Solution (see below)	10.0 ml
Heat-killed Bacterial Suspension (see below)	10.0 ml

 ATCC Medium 802:

Solution 1, Rye grass Cerophyll:
Cerophyll*	2.5 g
Distilled water	1.0 L

以5.0毫升的量配药。在121摄氏度下高压灭菌25分钟。倾斜将在溶液2上生长的细菌加入溶液1中，并在接种草履虫之前在30C下孵育24小时。


Vitamin Solution:

Calcium D-(+)-pantothenate	0.05 g
Nicotinamide	0.05 g
Pyridoxal . HCl	0.05 g
Pyridoxamine . HCl	0.025 g
Riboflavin	0.05 g
Folic acid	0.025 g
Thiamine . HCl	0.15 g
Biotin	0.0125 mg
DL-Thioctic acid	0.5 ml
Distilled water	100.0 ml

将10.0 ml维生素溶液（溶液会出现浑浊，在配制前剧烈搅拌）加入980 ml培养基802中。在121摄氏度下蒸15分钟。冷却并以10毫升等分试样的形式分配到T-25组织培养瓶中。在接种protist之前，向每个烧瓶中加入0.1ml热杀细菌悬浮液。

Heat-killed Bacterial Suspension: Prepare heat-killed Klebsiella pneumoniae subsp. pneumoniae ATCC 27889 in the following manner: 1. Inoculate a loopful of ATCC 27889 from a nutrient agar slant into 5 ml of nutrient broth. Incubate at 35C overnight. 2. Add 0.5 ml of bacterized broth prepared in step 1 to each of ten 1-L Erlenmeyer flasks, each containing 250 ml of Nutrient Broth. Incubate cultures at 35C for 24 hours. 3. Aseptically transfer bacterial suspensions to 500-ml sterilized screw-capped centrifuge bottles. Fill bottles with a maximum of 400 ml. Centrifuge in a refrigerated centrifuge at 5000 rpm for 10 minutes. 4. Decant supernatant and resuspend pellets in Page's Balanced Salt Solution (Medium 1323, see below). Pool all suspensions in a single bottle and centrifuge as in step 3. 5. Discard supernatant and resuspend pellet in Medium 1323 (final volume of cell suspension should be approximately 400 ml). 6. Repeat step 5 twice more. 7. Resuspend the final pellet in less than 100 ml of Medium 1323. Make sure cells are thoroughly suspended. 8. Transfer to a 125 ml screw-capped serum bottle and dilute to a final volume of 100 ml with Medium 1323. 9. Do a serial dilution of the suspension prepared in step 8. Carry dilution out to 10(-9) dilution. Plate 0.1 ml aliquots in triplicate from the 10(-7) - 10(-9) dilution tubes. Place the aliquots in the center of 100 mm petri plates containing nutrient agar and spread evenly over the surfaces with a spread bar. Incubate plates at 35C overnight. 10. Place bottle prepared in step 8 in a 60C water bath so that the liquid level of the water bath is above that of the suspension in the bottle. At 10-minute intervals swirl the bottle. Incubate for a total of 30 minutes. Allow the bottle to cool to room temperature. This treatment should kill all bacterial cells. 11. As a check for viable cells, add 3 drops of the cell suspension prepared in step 10 to the edge of a 100 mm petri plate containing nutrient agar. Hold the plate vertically to allow the drops to move to the opposite edge. Incubate plate at 35C for 48 hours. 12. Determine bacterial cell concentration from plates prepared in step 9. 13. Adjust the concentration of the heat-killed bacteria to 10(10) cells/ml.


ATCC Medium 1323:
Solution 1: Na2HPO4 ......................2.84 g
KH2PO4 .......................2.72 g
Distilled water............500.0 ml

在121摄氏度下高压灭菌20分钟。

Solution 2:
MgSO4 . 7H2O .................8.0 mg
CaCl2 . 2H2O .................8.0 mg
NaCl.........................0.240 g
Distilled water............500.0 mg