产品名称: |
TNM-FH培养基;TNM-FH培养基(无菌,液体),TNM-FH昆虫细胞培养基,TNM-FH昆虫培养基,TNMFH培养基 HyClone TNM-FH 培养基 |
---|---|
英文名称: | TNM-FH Insect Medium (sterile, liquid) |
培养基类型: | 昆虫细胞培养基 |
级别: | insect cell tested |
品牌: | HyClone |
产品目录号: | Sh30280.03 |
产品规格: | 1L |
颜色与澄清度: | 淡黄色透明液体,无任何沉淀。 |
保存条件: | 避光,2-8℃保存。 |
注意事项: | 避免呼入和皮肤接触。 |
相关产品: | -- |
TNM-FH昆虫细胞培养基产品描述:
TNM-FH培养基是一种改良的Grace昆虫细胞培养基,由Dr. W.F.Hink研制,最初用于培养甘蓝银纹夜蛾(Cabbage looper)x细胞系TN-368。现在该培养基用于培养多种鳞翅目物种衍生细胞系。TNM-FH昆虫培养基是在Grace培养基的基础上增加了乳白蛋白水解物和酵母提取物。乳白蛋白水解物提供各种游离氨基酸。酵母提取物作为维生素来源。当添加5-20%的胎牛血清时,该培养基能够支持多种鳞翅目昆虫细胞系的生长。
TNM-FH培养基配方与配制方法:
Ingredients | mg/L |
INORGANIC SALTS | |
Calcium chloride dihydrate | 1324.620 |
Magnesium chloride anhydrous | 1068.200 |
Magnesium sulphate anhydrous | 1357.660 |
Potassium chloride | 2240.000 |
Sodium dihydrogen phosphate anhydrous | 876.920 |
AMINO ACIDS | |
DL-Serine | 1100.000 |
Glycine | 650.000 |
L-Alanine | 225.000 |
L-Arginine hydrochloride | 700.000 |
L-Asparagine | 350.000 |
L-Aspartic acid | 350.000 |
L-Cystine dihydrochloride | 25.000 |
L-Glutamic acid | 600.000 |
L-Glutamine | 600.000 |
L-Histidine hydrochloride | 3377.640 |
L-Isoleucine | 50.000 |
L-Leucine | 75.000 |
L-Lysine hydrochloride | 625.000 |
L-Methionine | 50.000 |
L-Phenylalanine | 150.000 |
L-Proline | 350.000 |
L-Threonine | 175.000 |
L-Tryptophan | 100.000 |
L-Tyrosine disodium salt | 72.000 |
L-Valine | 100.000 |
ß-Alanine | 200.000 |
VITAMINS | |
Choline chloride | 0.200 |
D-Biotin | 0.010 |
D-Calcium-Pantothenate | 0.020 |
Folic acid | 0.020 |
Niacin | 0.020 |
Pyridoxine hydrochloride | 0.020 |
Riboflavin | 0.020 |
Thiamine hydrochloride | 0.020 |
myo-Inositol | 0.020 |
p-Amino benzoic acid (PABA) | 0.020 |
OTHERS | |
Alpha-Ketoglutaric acid | 370.000 |
D(+) Glucose | 700.000 |
D-Fructose | 400.000 |
Fumaric acid | 55.000 |
L-Malic acid | 670.000 |
Lactalbumin hydrolysate | 3330.000 |
Succinic acid | 60.000 |
Sucrose | 26680.000 |
Yeast extract | 3330.000 |
pH 6.2 |