| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141369 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 14489 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-nsp4 |
| 物种来源: | SARS-CoV-2 |
| 基因突变: | human codon optimized |
| GenBank编号 | JN247409 |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: |
pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141369 ; http://n2t.net/addgene:141369 ; RRID:Addgene_141369)
|
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro 10416 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10416)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 10416)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10416
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..1182
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 231..1173
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1130..1150
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 2734..2757
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 2794..2817
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 2829..3402
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(2996..3013)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3223..3242
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 3435..4034
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3435..3454)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3931..3951
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 4048..4636
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4101..4121)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4519..4530)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4843..5476
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5604..5620)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5604..5620)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5617..5639)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5628..5644
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5652..5682)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5697..5718
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5835..5852)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6006..6594)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6086..6105)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6765..7625)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7388..7407
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7626..7730)
/gene="bla"
/label=AmpR promoter
polyA_signal 7778..7912
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7815..7834)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7869..7888
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 8080..8713
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 8760..8885
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 9382..9615
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 9800..9844
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 10107..10222
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
ORIGIN
1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
1201 ttcgccgcca ccatgaagat tgtaaacaat tggcttaaac aacttattaa agtaaccctg
1261 gtattcttgt ttgtcgccgc aatcttttac ttgataacac cggtccacgt gatgtctaaa
1321 cacacagact ttagtagtga gataatcgga tataaagcta tcgatggcgg cgttacccgc
1381 gacatcgctt caaccgacac atgtttcgct aataagcacg ccgattttga cacttggttt
1441 agtcaaagag gaggaagcta cacaaacgac aaggcttgtc cgcttatcgc ggctgtaata
1501 acaagggaag ttgggtttgt cgtgcctggt ttgccaggta ctatactcag aaccacaaac
1561 ggtgactttc tgcacttctt gccaagggtt tttagcgcgg taggcaatat ctgctatacg
1621 ccaagcaagc tgattgaata caccgacttt gcgacgagcg catgtgtcct cgctgcagag
1681 tgtacgatat ttaaggatgc gagcggaaag cccgtacctt actgctatga tactaatgtc
1741 cttgagggat cagttgcgta tgagagtctt aggccggata cacgatatgt gctcatggat
1801 ggttccataa tccagttccc aaatacctac ctcgaagggt ccgttcgggt cgtcaccaca
1861 tttgatagcg aatattgcag acacggaaca tgcgaacgga gtgaagcagg ggtctgcgtc
1921 tccacaagtg ggcggtgggt cctcaataac gattattacc ggagcctgcc cggcgtgttt
1981 tgcggggtcg atgcggttaa cttgttgacg aatatgttta cgcctctcat acaacccatt
2041 ggcgcactgg atatatctgc aagtatagtc gctggaggca ttgtggcaat cgtggttacg
2101 tgtttggcgt attattttat gaggttccgc cgggctttcg gggaatattc ccacgtagtc
2161 gccttcaaca cactcttgtt tctcatgtct ttcactgtac tgtgtttgac tcctgtctac
2221 tctttcttgc cgggtgtata ctcagttatt tacttgtacc tcacgtttta tttgacaaac
2281 gatgtttcat ttctcgcgca tatccaatgg atggtgatgt ttacgccatt ggtgcctttc
2341 tggatcacga tcgcttatat catttgcatc agcaccaaac acttctattg gttctttagt
2401 aactacttga agcgcagggt tgtttttaac ggtgtcagct tctcaacgtt tgaagaggca
2461 gcactgtgta ctttccttct gaataaggaa atgtatctca agctgcggtc cgatgtcctg
2521 cttccgctta cccagtataa tcgctatctc gccctgtata ataagtacaa gtacttctca
2581 ggcgcgatgg atacaaccag ttatagagag gctgcctgct gccatcttgc caaagctttg
2641 aatgacttca gcaatagtgg ctcagacgtt ctctaccaac caccgcaaac ttccataacc
2701 tcagccgtac tccagctcga aggcggcggg ggatggagcc atccacaatt cgagaaaggc
2761 ggtggttcag gaggaggtag cgggggtgga tcatggtcac atccgcagtt tgaaaagtaa
2821 ggatcccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
2881 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
2941 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
3001 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
3061 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
3121 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
3181 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
3241 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
3301 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
3361 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa
3421 ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
3481 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
3541 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
3601 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
3661 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
3721 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
3781 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
3841 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
3901 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
3961 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
4021 agcccggtgc ctagacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
4081 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
4141 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
4201 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
4261 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
4321 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
4381 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
4441 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
4501 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
4561 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
4621 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
4681 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
4741 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
4801 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
4861 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
4921 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
4981 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
5041 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
5101 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
5161 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
5221 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
5281 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
5341 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
5401 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
5461 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
5521 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct
5581 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
5641 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
5701 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
5761 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
5821 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
5881 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
5941 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
6001 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
6061 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
6121 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
6181 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
6241 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
6301 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
6361 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
6421 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
6481 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
6541 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
6601 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
6661 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
6721 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
6781 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
6841 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
6901 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
6961 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
7021 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
7081 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
7141 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
7201 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
7261 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
7321 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
7381 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
7441 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
7501 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
7561 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
7621 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
7681 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
7741 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
7801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
7861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
7921 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
7981 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
8041 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
8101 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
8161 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
8221 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
8281 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
8341 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
8401 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
8461 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
8521 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
8581 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
8641 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
8701 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
8761 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
8821 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
8881 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
8941 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
9001 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
9061 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
9121 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
9181 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
9241 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
9301 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
9361 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
9421 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
9481 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
9541 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
9601 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
9661 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
9721 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
9781 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
9841 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
9901 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
9961 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
10021 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
10081 agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg gggtacagtg
10141 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
10201 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttatc
10261 gatgagtaat tcatacaaaa ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt
10321 aaactcccac taacgtagaa cccagagatc gctgcgttcc cgccccctca cccgcccgct
10381 ctcgtcatca ctgaggtgga gaagagcatg cgtgag
//
