| 质粒名称: | pMTMVi-M |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 118757 |
| 存储实验室: | Jose-Antonio Daros |
|
载体骨架基本信息 |
|
| 载体名称: | pUC18 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 3373 bp |
| 载体修饰: | -- |
| 载体类型: | 构建烟草花叶病毒重组克隆的中间产物 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Tobacco mosaic virus cDNA fragment with a cloning polylinker |
| 物种来源: | Tobacco mosaic virus |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMTMVi-M was a gift from Jose-Antonio Daros (Addgene plasmid # 118757 ; http://n2t.net/addgene:118757 ; RRID:Addgene_118757) |
| 参考文献部分: | Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Shi X, Cordero T, Garrigues S, Marcos JF, Daròs JA, Coca M.. Plant Biotech Journal (2018) 10.1111/pbi.13038 |
买家导航
pMTMViM载体基本信息
pMTMViM载体图谱质粒图谱和多克隆位点信息
pMTMViM载体简介
pMTMViM载体序列
LOCUS pMTMVi-M 3373 bp ds-DNA circular SYN 19-DEC-2018
DEFINITION Intermediate plasmid to build tobacco mosaic virus recombinant
clones that move systemically throughout the plant.
ACCESSION .
VERSION .
KEYWORDS pMTMVi-M
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3373)
AUTHORS Shi X, Cordero T, Garrigues S, Marcos JF, Daros JA, Coca M.
TITLE Efficient production of antifungal proteins in plants using a new
transient expression vector derived from tobacco mosaic virus.
JOURNAL Plant Biotech Journal (2018)
REFERENCE 2 (bases 1 to 3373)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 19, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3373
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 368..389
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 404..434
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 442..458
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 447..469
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 466..482
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 466..482
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 478..783
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSAQLTLTKGNKSWVPNSPYSESYYARSLAVVLQRRDWEN
PGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDALEVLQLEVVKMHNK"
primer_bind 501..521
/label=T3
/note="T3 promoter, forward primer"
promoter 503..521
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(547..566)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(548..566)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(576..593)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(576..592)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(585..607)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin complement(1864..2452)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1944..1963)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2464..3324)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3087..3106
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 atggtctcga agattacaaa cgtgagagac ggagggccca tggaacttac agaagaagtc
61 gttgatgagt tcatggaaga tgtccctatg tcgatcaggc ttgcaaagtt tcgatctcga
121 accggaaaaa agagtgatgt ccgcaaaggg aaaaatagta gtagtgatcg gtcagtgccg
181 aacaagaact atagaaatgt taaggatttt ggaggaatga gttttaaaaa gaataattta
241 atcgatgatg attcggaggc tactgtcgcc gaatcggatt cgttttaaat agatcttaca
301 gtatcactac tccatctcag ttcgtgttct tgtcaccggt gaaagcgggc agtgagcgca
361 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgctcc
421 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
481 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctgggtaccc
541 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
601 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
661 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
721 aatggcgaat gggacgcgct cgaggtcctg caacttgagg tagtcaagat gcataataaa
781 taacggattg tgtccgtaat cacacgtggt gcgtacgata acgcatagtg tttttccctc
841 cacttaaatc gaagggttgt gtcttggatc gcgcgggtca aatgtatatg gttcatatac
901 atccgcaggc acgtaataaa gcgaggggtt cggaaccaaa tcctcaaaaa gaggtccgaa
961 aaataataat aatttaggta aggggcgttc aggcggaagg cctaaaccaa aaagttttga
1021 tgaagttgaa aaagagtttg ataatttgat tgaagatgaa gccgagacgt cggtcgcgga
1081 ttctgattcg tattaaatat gtcttactca atcacttctc catcgcaatt tgtgtttttg
1141 tcatctgtat gggctgaccc tatagaattg ttaaacgttt gtacaaattc gttaggtaac
1201 cagtttcaaa cacagcaagc aagaactact gttcaacagc agttcagcga ggtgtggaaa
1261 cctttccctc agagcaccgt cagatttcct ggcgatgttt ataaggtgta caggtacaat
1321 gcagttttag atcctctaat tactgcgttg ctggggtctt tcgatactag gaatagaata
1381 atcgaagtag aaaaccagca gaatccgaca acagctgaaa cgttagatgc tacccgcagg
1441 gtagacgacg ctacggttgc aattcggtct gctataaata atttagttaa tgaactagta
1501 agaggtactg gactgtacaa tcaaaatact tttgaaagta tgtctgggtt ggtctggacc
1561 tctgcacctg catcttaaat gcataggtgc tgaaatataa agtttgtgtt tctaaaacac
1621 acgtggtacg tacgataacg tatagtgttt ttccctccac ttaaatcgaa gggtagtgtc
1681 ttggagcgcg cggagtaaac atatatggtt catatatgtc cgtaggcacg taaaaaagcg
1741 agggattcga attcccccgg aacccccggg ttggggccca gggtcggcat ggcatctcca
1801 cctcctcgcg gtccgacctg ggctacttcg gtaggctaag ggggagaccg cgttgctggc
1861 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
1921 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
1981 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
2041 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
2101 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
2161 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
2221 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
2281 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
2341 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
2401 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
2461 tttttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
2521 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
2581 ccccagtgct gcaatgatac cgcgagagcc acgctcaccg gctccagatt tatcagcaat
2641 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
2701 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
2761 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
2821 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
2881 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
2941 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
3001 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
3061 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
3121 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
3181 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
3241 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
3301 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
3361 gggttattgt ctc
//
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