| 质粒名称: | p21-Reporter |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 52432 |
| 存储实验室: | Joel Voldman |
|
载体骨架基本信息 |
|
| 载体名称: | pTurboRFP-PRL |
| 载体出品公司: | Evrogen |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4127 bp |
| 完整质粒大小: | 4794 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | p21-p53 Tandem Response Element Promoter |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 667 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | 13X repeated p21-p53 response element |
| 标签/融合蛋白: | TurboRFP（主干上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | SV40pro_F_primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p21-Reporter was a gift from Joel Voldman (Addgene plasmid # 52432 ; http://n2t.net/addgene:52432 ; RRID:Addgene_52432) |
| 参考文献部分: | Cell-based biosensor to report DNA damage in micro- and nanosystems. Fendyur A, Varma S, Lo CT, Voldman J. Anal Chem. 2014 Aug 5;86(15):7598-605. doi: 10.1021/ac501412c. Epub 2014 Jul 7. 10.1021/ac501412c PubMed 25001406 |
买家导航
p21Reporter载体基本信息
p21Reporter载体图谱质粒图谱和多克隆位点信息
p21Reporter载体简介
p21Reporter载体序列
LOCUS p21-Reporter 4741 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION DNA damage inducible Red Fluorescent Protein for mammalian
expression .
ACCESSION .
VERSION .
KEYWORDS p21-Reporter
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4741)
AUTHORS Fendyur A, Varma S, Lo CT, Voldman J
TITLE Cell-based biosensor to report DNA damage in micro- and nanosystems.
JOURNAL Anal Chem. 2014 Aug 5;86(15):7598-605. doi: 10.1021/ac501412c. Epub
2014 Jul 7.
PUBMED 25001406
REFERENCE 2 (bases 1 to 4741)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4741
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 802..1497
/codon_start=1
/product="red fluorescent protein from Entacmaea
quadricolor"
/label=TurboRFP
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
DLPSKLGHR"
polyA_signal 1620..1741
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1657..1676)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1711..1730
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1748..2203)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1885..1906)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2097..2116
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2230..2334
/gene="bla"
/label=AmpR promoter
promoter 2336..2693
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2359..2379)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2544..2679
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2606..2625
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2728..3522
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2782..2801)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3392..3411
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3710..3729)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3754..3801
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4130..4718
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4619..4638
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
61 ttctgtggat aaccgtatta ccgccatgca ttagttatta ctagcgctac cggactcaga
121 tctcgagctc aagccaacat gttgggacat gttcagggcg ctggcaagtg gcaacatcgt
181 ggaacaacat gttgggacat gttcgatcac gctgcgcgcg gccaaatggc cgtacaacat
241 gttgggacat gttcacacac ccgccgcgct gcgttttctg gatacaacat gttgggacat
301 gttcagccgc tacagggcgc gtcagattcg cagccaacat gttgggacat gttcacgtgg
361 cactgttcgc gcaacatcgc cttacaacat gttgggacat gttcatgtgc gcggaacccc
421 tattctatcg cctacaacat gttgggacat gttcgccata ccacatttgg caagaccgac
481 caaacaacat gttgggacat gttcagtcca cacctccccc gtcagcgacg cccacaacat
541 gttgggacat gttcaccgtc aacagcgcgt catccattcc accgcaacat gttgggacat
601 gttcaattcg cgttaaattt ttgtgtacag gttgcaacat gttgggacat gttcgcgctc
661 agctcatttt tgcggtcatc gatacaacat gttgggacat gttcaggagt atcctccagc
721 gcggggatct gtcacaacat gttgggacat gttcacaact gggtatataa ggtacgggcc
781 cgggatccac cggtcgccac catgagcgag ctgatcaagg agaacatgca catgaagctg
841 tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag
901 ccctacgagg gcacccagac catgaagatc aaggtggtcg agggcggccc tctccccttc
961 gccttcgaca tcctggctac cagcttcatg tacggcagca aagccttcat caaccacacc
1021 cagggcatcc ccgacttctt taagcagtcc ttccctgagg gcttcacatg ggagagaatc
1081 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagctt ccagaacggc
1141 tgcatcatct acaacgtcaa gatcaacggg gtgaacttcc catccaacgg ccctgtgatg
1201 cagaagaaaa cacgcggctg ggaggccaac accgagatgc tgtaccccgc tgacggcggc
1261 ctgagaggcc acagccagat ggccctgaag ctcgtgggcg ggggctacct gcactgctcc
1321 ttcaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcttccac
1381 ttcgtggacc acagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag
1441 cacgagatgg ctgtggccaa gtactgcgac ctccctagca aactggggca cagatgatgc
1501 ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
1561 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
1621 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
1681 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
1741 aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
1801 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
1861 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
1921 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
1981 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
2041 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
2101 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
2161 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
2221 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
2281 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
2341 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
2401 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
2461 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
2521 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
2581 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
2641 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
2701 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
2761 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
2821 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
2881 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
2941 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
3001 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
3061 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
3121 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
3181 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
3241 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
3301 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
3361 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
3421 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
3481 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
3541 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
3601 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
3661 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
3721 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
3781 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
3841 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
3901 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
3961 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
4021 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
4081 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
4141 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
4201 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
4261 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
4321 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
4381 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
4441 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
4501 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
4561 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
4621 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
4681 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
4741 t
//
