| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.74 ; pGL4.74[hRluc/TK] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | HSV-TK |
| 载体大小: | 4237 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | hRluc |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.74[hRluc/TK]载体是信号通路报告载体；含海肾荧光素酶报告基因hRluc。 |
| 产品目录号: | E6921 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.74[hRluc/TK]载体pGL4.74质粒基本信息
pGL4.74[hRluc/TK]质粒图谱pGL4.74载体图谱和pGL4.74[hRluc/TK]载体序列pGL4.74质粒序列多克隆位点信息
pGL4.74[hRluc/TK]质粒pGL4.74载体简介
pGL4.74载体简介 The pGL4.74[hRluc/TK] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.74[hRluc/TK] Vector contains the hRluc luciferase reporter gene and an HSV-TK promoter and can be used as an expression control or a coreporter vector.
pGL4.74[hRluc/TK]质粒序列pGL4.74载体序列
LOCUS AY738230 4237 bp DNA circular SYN 08-NOV-2004
DEFINITION Renilla luciferase reporter vector pGL4.74[hRluc/TK], complete
sequence.
ACCESSION AY738230
VERSION AY738230.1 GI:55535642
KEYWORDS .
SOURCE Renilla luciferase reporter vector pGL4.74[hRluc/TK]
ORGANISM Renilla luciferase reporter vector pGL4.74[hRluc/TK]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4237)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
Corporation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4237)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2004) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..4237
/organism="Renilla luciferase reporter vector
pGL4.74[hRluc/TK]"
/mol_type="other DNA"
/db_xref="taxon:296667"
regulatory 27..779
/regulatory_class="promoter"
/note="HSV-TK"
CDS 815..1750
/codon_start=1
/transl_table=11
/product="luciferase hRluc"
/protein_id="AAV52885.1"
/db_xref="GI:55535643"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
misc_feature 1784..2005
/note="SV40 late poly(A) region"
primer_bind 2071..2090
/note="reporter vector primer 4 (RVPrimer4)"
rep_origin 2330
/note="ColE1-derived"
gene complement(3119..3979)
/gene="ampR"
CDS complement(3119..3979)
/gene="ampR"
/note="AmpR"
/codon_start=1
/transl_table=11
/product="synthetic beta-lactamase"
/protein_id="AAV52886.1"
/db_xref="GI:55535644"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4084..4237
/regulatory_class="polyA_signal_sequence"
/note="synthetic; transcriptional pause site"
primer_bind complement(4186..4205)
/note="reporter vector primer 3 (RVPrimer3)"
ORIGIN
1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
661 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
781 agcttggcaa tccggtactg ttggtaaagc caccatggct tccaaggtgt acgaccccga
841 gcaacgcaaa cgcatgatca ctgggcctca gtggtgggct cgctgcaagc aaatgaacgt
901 gctggactcc ttcatcaact actatgattc cgagaagcac gccgagaacg ccgtgatttt
961 tctgcatggt aacgctgcct ccagctacct gtggaggcac gtcgtgcctc acatcgagcc
1021 cgtggctaga tgcatcatcc ctgatctgat cggaatgggt aagtccggca agagcgggaa
1081 tggctcatat cgcctcctgg atcactacaa gtacctcacc gcttggttcg agctgctgaa
1141 ccttccaaag aaaatcatct ttgtgggcca cgactggggg gcttgtctgg cctttcacta
1201 ctcctacgag caccaagaca agatcaaggc catcgtccat gctgagagtg tcgtggacgt
1261 gatcgagtcc tgggacgagt ggcctgacat cgaggaggat atcgccctga tcaagagcga
1321 agagggcgag aaaatggtgc ttgagaataa cttcttcgtc gagaccatgc tcccaagcaa
1381 gatcatgcgg aaactggagc ctgaggagtt cgctgcctac ctggagccat tcaaggagaa
1441 gggcgaggtt agacggccta ccctctcctg gcctcgcgag atccctctcg ttaagggagg
1501 caagcccgac gtcgtccaga ttgtccgcaa ctacaacgcc taccttcggg ccagcgacga
1561 tctgcctaag atgttcatcg agtccgaccc tgggttcttt tccaacgcta ttgtcgaggg
1621 agctaagaag ttccctaaca ccgagttcgt gaaggtgaag ggcctccact tcagccagga
1681 ggacgctcca gatgaaatgg gtaagtacat caagagcttc gtggagcgcg tgctgaagaa
1741 cgagcagtaa ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat
1801 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
1861 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
1921 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
1981 gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag
2041 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
2101 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
2161 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
2221 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
2281 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
2341 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
2401 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
2461 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
2521 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
2581 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
2641 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
2701 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
2761 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
2821 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
2881 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
2941 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
3001 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
3061 agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt
3121 accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt tcgtccatag
3181 tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca tcaggcccca
3241 gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca gcaatgaacc
3301 agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc tccatccagt
3361 ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt ttccgaagag
3421 ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg gcttcgttca
3481 actctggttc ccagcggtca agccgggtca catgatcacc catattatga agaaatgcag
3541 tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca
3601 tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga tgcttttccg
3661 tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga ccaagctgct
3721 cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca
3781 tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca
3841 gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg
3901 tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac
3961 gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt tatcagggtt
4021 actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta ttggacaggc
4081 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
4141 gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct
4201 gtccccagtg caagtgcagg tgccagaaca tttctct
//
