| 质粒名称: | pAG001 His6-TwinStrep-SUMO-AapCas12b |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 153162 |
| 存储实验室: | Omar Abudayyeh, Jonathan Gootenberg |
|
载体骨架基本信息 |
|
| 载体名称: | SUMO expression backbone |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 9473 bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SUMO expression backbone |
|
文献引用方法 |
|
| 材料和方法部分: | pAG001 His6-TwinStrep-SUMO-AapCas12b was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 153162 ; http://n2t.net/addgene:153162 ; RRID:Addgene_153162) |
| 参考文献部分: | Point-of-care testing for COVID-19 using SHERLOCK diagnostics. Joung J, Ladha A, Saito M, Segel M, Bruneau R, Huang MW, Kim NG, Yu X, Li J, Walker BD, Greninger AL, Jerome KR, Gootenberg JS, Abudayyeh OO, Zhang F. medRxiv. 2020 May 8. doi: 10.1101/2020.05.04.20091231. |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAG001 His6-TwinStrep-SUMO-AapCas12b 9473 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Bacterial expression plasmid for AapCas12b.
ACCESSION .
VERSION .
KEYWORDS pAG001 His6-TwinStrep-SUMO-AapCas12b
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9473)
AUTHORS Joung J, Ladha A, Saito M, Segel M, Bruneau R, Huang MW, Kim NG, Yu
X, Li J, Walker BD, Greninger AL, Jerome KR, Gootenberg JS,
Abudayyeh OO, Zhang F
TITLE Point-of-care testing for COVID-19 using SHERLOCK diagnostics.
JOURNAL medRxiv. 2020 May 8. doi: 10.1101/2020.05.04.20091231.
PUBMED 32511521
REFERENCE 2 (bases 1 to 9473)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9473
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 1..20
/label=T7
/note="T7 promoter, forward primer"
promoter 1..19
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 20..44
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 59..81
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 100..117
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 127..144
/codon_start=1
/product="thrombin recognition and cleavage site"
/label=thrombin site
/translation="LVPRGS"
CDS 157..240
/codon_start=1
/product="two Strep-Tag(R)II moieties connected by a linker
for enhanced binding to Strep-Tactin(R), an engineered form
of streptavidin (Schmidt et al., 2013)"
/label=Twin-Strep-tag
/translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
CDS 241..531
/codon_start=1
/gene="S. cerevisiae SMT3 (truncated)"
/product="cleavable ubiquitin-like protein tag"
/label=SUMO
/translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"
primer_bind complement(3974..3992)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 3988..4035
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(4210..4238)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(4265..4283)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4351..4455
/gene="bla"
/label=AmpR promoter
CDS 4456..5316
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4674..4693)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5487..6075
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5976..5995
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6229..6246
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 6261..6400
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(6326..6345)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6486..6508
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(6502..6693)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(8005..9087)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 9049..9068
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(9088..9165)
/gene="lacI"
/label=lacI promoter
primer_bind 9371..9390
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
121 agcggcctgg tgccgcgcgg cagccatatg gctagctgga gccatccgca gtttgaaaaa
181 ggtggtggta gcggtggtgg ttcaggtggt agtgcatggt cacaccctca gtttgagaaa
241 atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct
301 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa
361 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg
421 gactccttaa gattcttgta cgacggtatt agaatccaag ctgatcagac ccctgaagat
481 ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggatccatg
541 gccgtaaaat ctatgaaagt taagttgcgt ctggacaaca tgccagaaat tcgcgctggc
601 ctttggaaac tgcacaccga ggtcaatgca ggcgttcgtt attataccga gtggttaagt
661 ttattgcgtc aggaaaattt ataccgtcgc agtccaaacg gggacgggga acaggagtgt
721 tacaagacag ctgaggagtg caaggcagag ttgcttgagc gcttgcgcgc acgccaagtt
781 gagaatggac actgcggtcc ggctgggtca gacgatgagc ttcttcaatt ggcacgtcag
841 ttatacgaat tattagtacc ccaggcgatc ggtgcgaaag gcgacgctca gcagatcgcg
901 cgtaaattcc tgtccccgct ggctgataaa gatgctgtcg gtggcctggg aattgccaaa
961 gccgggaata aaccgcgttg ggtccgtatg cgcgaggctg gagagccggg atgggaagaa
1021 gagaaggcaa aagcggaagc ccgtaaatcc acggaccgta ctgcagacgt cttgcgcgcc
1081 ttagccgatt ttgggcttaa accgttgatg cgcgtctaca ccgactcaga tatgtcgtct
1141 gttcaatgga agcccctgcg taaagggcag gccgtacgta cctgggatcg tgatatgttt
1201 cagcaggcga tcgagcgtat gatgtcctgg gaatcctgga accaacgcgt aggcgaagca
1261 tacgcaaaat tggtcgaaca aaagtcgcgc ttcgagcaga agaactttgt cggacaggaa
1321 catctggttc aattggtcaa ccagctgcaa caggacatga aggaagcatc tcacggtctg
1381 gaatcgaaag agcaaactgc acactatctt actggtcgtg cgttgcgtgg tagtgataaa
1441 gtgtttgaga agtgggagaa gcttgatccc gatgcgcctt ttgatcttta cgacaccgag
1501 attaagaacg ttcaacgtcg caatacacgt cgctttggtt cgcatgactt attcgccaag
1561 ttggcagaac cgaaatacca agccctgtgg cgtgaagacg cgagctttct tacgcgttat
1621 gcagtgtaca atagtattgt ccgcaaactg aaccacgcca aaatgtttgc aacctttacg
1681 cttccagacg ccacagctca ccctatttgg acccgctttg ataaattggg gggtaatctt
1741 caccaatata cctttctgtt caacgagttc ggtgagggtc gccatgctat ccgcttccaa
1801 aagttactga cggtggaaga cggcgtggca aaggaagttg atgacgtgac tgtcccgatc
1861 agcatgtcag cccagttaga cgacctgctg ccgcgcgatc cgcatgaatt ggtcgcgctt
1921 tatttccaag attatggcgc ggaacaacac ttagcgggtg aattcggtgg ggcaaaaatc
1981 caatatcgtc gcgaccagct taaccacctg cacgctcgcc gcggtgcccg cgatgtttat
2041 ctgaaccttt cggtacgtgt gcagagtcaa tcagaggcac gcggcgaacg tcgtcctcca
2101 tatgctgcag tctttcgctt ggtaggggat aatcaccgcg catttgtgca tttcgataag
2161 ttatctgact accttgcaga acatccggat gatggtaaat tgggctcgga gggtctttta
2221 tcaggcttac gcgtaatgag tgtagacctg ggcctgcgta cttcagccag catctctgta
2281 tttcgcgtcg cacgtaaaga cgagttaaaa ccgaattccg agggtcgcgt tcctttctgc
2341 ttccctattg aagggaacga gaacctggta gcggttcacg aacgctccca attgttgaag
2401 ctgcccggag agacagaatc gaaggatctg cgtgctatcc gtgaggagcg ccagcgtacg
2461 ttgcgccaat tacgtacaca gcttgcatat ttgcgtcttt tagtacgctg tggttcagag
2521 gatgtgggtc gccgtgaacg ttcctgggca aagctgatcg aacaaccgat ggatgctaat
2581 cagatgaccc ccgactggcg cgaagctttc gaggacgagc tgcagaaact taagtcattg
2641 tatggtatct gtggagatcg tgaatggact gaggcagtct acgaatctgt gcgccgcgta
2701 tggcgccata tgggaaagca ggtacgcgac tggcgtaagg atgttcgttc cggcgaacgc
2761 cctaagattc gcggctatca gaaagacgtt gttggtggca attcgattga acagattgag
2821 tatcttgaac gtcagtacaa atttctgaag tcttggtcat tctttggaaa agtatcaggt
2881 caggtaattc gtgccgagaa ggggtcccgc ttcgctatca ccttacgcga acatatcgat
2941 catgcgaaag aagaccgcct taaaaaattg gctgatcgta tcatcatgga ggcattagga
3001 tatgtgtatg cactggacga cgaacgtggc aaagggaaat gggttgccaa gtatccgccg
3061 tgtcaattga ttttactgga agaattgtcg gagtaccagt ttaacaacga ccgtcccccc
3121 tcagagaata accagcttat gcaatggtcg catcgtggag tgttccaaga actgttaaac
3181 caagcacaag ttcatgacct tcttgtaggt actatgtatg cggcattttc cagtcgcttt
3241 gacgcacgta cgggggctcc ggggatccgt tgtcgtcgcg tacctgcacg ttgcgcacgc
3301 gaacagaatc cggagccttt tccttggtgg ttgaataagt tcgttgcgga gcataagttg
3361 gacggctgcc ctttgcgcgc tgatgacctg atccccacag gcgaaggtga gttcttcgtt
3421 agtccatttt cagctgagga gggtgacttt caccagatcc atgccgacct taacgccgcc
3481 caaaatttgc aacgtcgtct ttggtcagac tttgatatct cgcaaattcg tctgcgctgt
3541 gattgggggg aggttgatgg cgagccagtt ttaattccgc gcacaactgg caagcgtacc
3601 gctgacagct acggcaacaa ggttttttat accaagaccg gtgtaacgta ttacgaacgt
3661 gagcgcggta aaaaacgtcg caaagttttt gctcaggaag aactgagtga agaagaggct
3721 gaacttttgg tggaagccga tgaggcccgt gagaaatcag tagttttgat gcgtgatccg
3781 tcaggaatca ttaatcgtgg agactggacg cgtcaaaaag aattttggag tatggtcaac
3841 caacgcatcg agggttacct tgttaagcag atccgctcgc gcgtccgtct gcaggaaagt
3901 gcttgtgaga acactgggga catctgagcg gccgcactcg aggcccgaaa ggaagctgag
3961 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
4021 ttgaggggtt ttttgctgaa aggaggaact atatccggat atcccgcaag aggcccggca
4081 gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga
4141 tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata
4201 aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
4261 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
4321 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
4381 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
4441 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
4501 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
4561 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
4621 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
4681 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
4741 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
4801 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
4861 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
4921 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
4981 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
5041 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
5101 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
5161 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
5221 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
5281 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
5341 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
5401 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
5461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
5521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
5581 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
5641 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
5701 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
5761 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
5821 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
5881 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
5941 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
6001 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
6061 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
6121 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
6181 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
6241 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
6301 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
6361 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
6421 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
6481 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
6541 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
6601 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
6661 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
6721 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
6781 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
6841 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
6901 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
6961 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
7021 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
7081 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
7141 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct
7201 gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt
7261 tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg
7321 ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg
7381 cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag
7441 gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc
7501 gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc
7561 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc
7621 cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg
7681 ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg
7741 agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga
7801 aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg
7861 ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag
7921 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc
7981 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
8041 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag
8101 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
8161 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
8221 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac
8281 cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc
8341 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat
8401 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat
8461 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact
8521 taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc
8581 cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac
8641 atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc
8701 atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc
8761 cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag
8821 ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact
8881 ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt
8941 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac
9001 gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc
9061 gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg
9121 ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct
9181 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca
9241 ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
9301 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
9361 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
9421 tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
//
