| 质粒名称: | pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 193942 |
| 存储实验室: | Greg Delgoffe |
|
载体骨架基本信息 |
|
| 载体名称: | MSGV-1D3-28Z All ITAMs intact |
| 载体出品公司: | Steven Rosenberg |
| 空载体大小: | 7013bp |
| 完整质粒大小: | 7547bp |
| 载体类型: | Retroviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv) |
| 物种来源: | Synthetic |
| 插入大小: | 719bp |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | TACACAGTCCTGCTGACCA |
| 3’测序引物 | cgtaacagaacagcacgc |
|
文献引用方法 |
|
| 材料和方法部分: | pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1 was a gift from Greg Delgoffe (Addgene plasmid # 193942 ; http://n2t.net/addgene:193942 ; RRID:Addgene_193942) |
| 参考文献部分: | Fully murine CD105-targeted CAR-T cells provide an immunocompetent model for CAR-T cell biology. Lontos K, Wang Y, Colbert M, Kumar A, Joshi S, Philbin M, Wang Y, Frisch A, Lohmueller J, Rivadeneira DB, Delgoffe GM. Oncoimmunology. 2022 Oct 18;11(1):2131229. doi: 10.1080/2162402X.2022.2131229. eCollection 2022. 10.1080/2162402X.2022.2131229 PubMed 36275862 |
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pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1载体质粒基本信息
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1质粒图谱载体图谱和pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1载体序列质粒序列多克隆位点信息
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1质粒载体简介
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1质粒序列载体序列
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1载体图谱质粒图谱pdf版和pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1载体序列质粒序列等相关资料下载
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1质粒载体应用举例
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