| 质粒名称: | pHR_EGFPligand |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 79129 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EGFP surface ligand |
| 物种来源: |
Synthetic
|
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_EGFPligand was a gift from Wendell Lim (Addgene plasmid # 79129 ; http://n2t.net/addgene:79129 ; RRID:Addgene_79129) |
| 参考文献部分: | Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA. Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. Epub 2016 Jan 28. 10.1016/j.cell.2016.01.012 PubMed 26830878 |
买家导航
pHREGFPligand载体基本信息
pHREGFPligand载体图谱质粒图谱和多克隆位点信息
pHREGFPligand载体简介
pHREGFPligand载体序列
LOCUS pHR_EGFPligand 9979 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral vector for surface expressed EGFP (SFFV promoter).
ACCESSION .
VERSION .
KEYWORDS pHR_EGFPligand
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9979)
AUTHORS Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim
WA
TITLE Engineering Customized Cell Sensing and Response Behaviors Using
Synthetic Notch Receptors.
JOURNAL Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012.
Epub 2016 Jan 28.
PUBMED 26830878
REFERENCE 2 (bases 1 to 9979)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9979
/organism="synthetic DNA construct"
/mol_type="other DNA"
intron 655..720
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 850..870
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 1295..1429
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1332..1351)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1386..1405
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 1597..2230
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 2277..2402
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 2899..3132
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 3317..3361
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 3646..3763
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 3916..4323
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
regulatory 4391..4400
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4460..5176
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 5439..6027
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5492..5512)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5910..5921)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6030..6046)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6031..6047)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 6137..6370
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(6395..6413)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(6396..6413)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(6703..6722)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(6936..6955)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7055..7077
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7115..7133)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7201..7305
/gene="bla"
/label=AmpR promoter
CDS 7306..8166
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7524..7543)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8337..8925
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8826..8845
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9079..9096
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(9166..9186)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9171..9500
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9351..9486
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9413..9432
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
ORIGIN
1 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
61 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
121 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
181 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
241 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
301 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
361 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
421 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
481 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
541 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
601 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
661 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
721 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
781 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
841 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
901 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
961 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
1021 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
1081 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
1141 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
1201 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
1261 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
1321 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
1381 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
1441 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
1501 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
1561 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
1621 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
1681 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
1741 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
1801 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
1861 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
1921 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
1981 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
2041 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
2101 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
2161 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
2221 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
2281 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
2341 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
2401 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
2461 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
2521 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
2581 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
2641 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
2701 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
2761 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
2821 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
2881 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
2941 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
3001 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
3061 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
3121 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
3181 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
3241 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
3301 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
3361 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
3421 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
3481 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
3541 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
3601 gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg
3661 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
3721 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
3781 cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa
3841 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
3901 ggcaagctag ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata
3961 gagaagttca gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat
4021 ctgcggtgag cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg
4081 gccccggccc gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc
4141 ttaagaccca tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat
4201 ttgaattaac caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc
4261 tataaaagag ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc
4321 gggggggatc tggagctctc gagaattctc acgcgtcaag tggagcaagg caggtggaca
4381 gtggatcgcc gccaccatgg agaccgacac cctcttgctc tgggtcctgc tgctgtgggt
4441 tccgggtagt accggagaca tggtttctaa gggtgaagaa ttgttcaccg gagttgtgcc
4501 catcctggtt gagctggacg gagacgttaa cggacacaag ttctcagtga gcggcgaagg
4561 agagggcgac gctacgtacg gtaaactgac gttgaagttc atctgtacaa caggaaagct
4621 gccagtgccc tggcctacac tcgtgaccac cctcacctac ggagtccagt gcttctcccg
4681 gtaccccgac cacatgaaac agcacgattt cttcaagtcc gcgatgcctg agggctatgt
4741 tcaggaacgc accatattct tcaaggacga cggtaactat aaaaccaggg ctgaagttaa
4801 gttcgagggg gacacgttgg taaatagaat tgagttgaaa ggaatcgatt tcaaagaaga
4861 cggcaatatt ttgggccaca agctggagta caactacaac agccacaacg tgtatattat
4921 ggctgataag cagaagaacg gcatcaaggt gaacttcaaa atccgacaca atatcgagga
4981 tggctctgtg caactggccg accattacca gcaaaacacg cctatcggcg acggtccggt
5041 tttgctgcca gataaccact acctctccac gcaatcagcc ctctccaagg atcctaacga
5101 aaaaagagac catatggttc tgcttgaatt cgtgacggct gctgggatca ccctgggaat
5161 ggatgagctg tataagaacg ccgtgggcca ggatacgcag gaggtgatcg tcgtgcccca
5221 cagcttgccc ttcaaggtcg tggttattag tgctatcctg gcgttggtgg tgctgactat
5281 tatctccctt atcatactta tcatgctgtg gcaaaaaaaa ccccgataag atccttgact
5341 tgcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac tctagagtcg
5401 acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa
5461 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
5521 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
5581 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
5641 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
5701 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
5761 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
5821 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
5881 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
5941 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
6001 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg
6061 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
6121 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
6181 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
6241 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
6301 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
6361 atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt
6421 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc
6481 ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg
6541 tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg
6601 aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca
6661 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
6721 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
6781 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
6841 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
6901 cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag
6961 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
7021 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
7081 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
7141 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
7201 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
7261 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
7321 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
7381 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
7441 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
7501 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
7561 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
7621 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
7681 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
7741 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
7801 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
7861 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
7921 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
7981 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
8041 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
8101 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
8161 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
8221 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
8281 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
8341 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
8401 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
8461 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
8521 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
8581 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
8641 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
8701 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
8761 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
8821 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
8881 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
8941 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
9001 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
9061 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
9121 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt
9181 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
9241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
9301 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
9361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
9421 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
9481 gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga
9541 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
9601 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
9661 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
9721 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
9781 caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc
9841 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
9901 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
9961 atgacctggt ggataccgg
//
