| 质粒名称: | pGOAL19 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 20190 |
| 存储实验室: | Tanya Parish |
|
载体骨架基本信息 |
|
| 载体名称: | pBR322 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2500bp |
| 完整质粒大小: | -- |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pGOAL19 was a gift from Tanya Parish (Addgene plasmid # 20190 ; http://n2t.net/addgene:20190 ; RRID:Addgene_20190) |
| 参考文献部分: | Use of a flexible cassette method to generate a double unmarked Mycobacterium tuberculosis tlyA plcABC mutant by gene replacement. Parish T, Stoker NG. Microbiology. 2000 Aug . 146 ( Pt 8)():1969-75. 10.1099/00221287-146-8-1969 PubMed 10931901 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGOAL19 10435 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pGOAL19
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10435)
AUTHORS Parish T, Stoker NG
TITLE Use of a flexible cassette method to generate a double unmarked
Mycobacterium tuberculosis tlyA plcABC mutant by gene replacement.
JOURNAL Microbiology. 2000 Aug . 146 ( Pt 8)():1969-75.
PUBMED 10931901
REFERENCE 2 (bases 1 to 10435)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10435
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 84..103
/label=T7
/note="T7 promoter, forward primer"
promoter 84..102
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
gap 273..772
/estimated_length=500
CDS complement(2728..5775)
/codon_start=1
/gene="lacZ"
/product="beta-galactosidase"
/label=lacZ
/translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
SWSPSVSAEFQLSAGRYHYQLVWCQK"
primer_bind 5751..5773
/label=M13/pUC Forward
/note="In lacZ gene"
promoter complement(6065..6083)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(6066..6083)
/label=SP6
/note="SP6 promoter, forward primer"
terminator 6307..6350
/label=bacterial terminator
/note="putative bacterial transcription terminator"
gap 7630..7882
/estimated_length=253
promoter 7965..7993
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(8176..8198)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 8284..8424
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 8339..8358
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(8439..8456)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8610..9198)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8690..8709)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9369..10229)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9992..10011
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10230..10334)
/gene="bla"
/label=AmpR promoter
primer_bind 10402..10420
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 aattcttaat taagcggccg cggtacccaa aaaaagcccg ctcattaggc gggctaattc
61 gcctcgaggt ggcttatcga aattaatacg actcactata gggagaccgg aagcttcacg
121 tggtcgacgg tatcgataag cttgatatcg aattcctgca gcccggggga tcgaaaaggt
181 taggaatacg gttagccatt tgcctgcttt tatatagtta tatgggattc acctttatgt
241 tgataagaaa taaaagaaaa tgccaatagg atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcggcat
781 tttcttttgc gtttttattt gttaactgtt aattgtcctt gttcaaggat gctgtctttg
841 acaacagatg ttttcttgcc tttgatgttc agcaggaagc ttggcgcaaa cgttgattgt
901 ttgtctgcgt agaatcctct gtttgtcata tagcttgtaa tcacgacatt gtttcctttc
961 gcttgaggta cagcgaagtg tgagtaagta aaggttacat cgttaggatc aagatccatt
1021 tttaacacaa ggccagtttt gttcagcggc ttgtatgggc cagttaaaga attagaaaca
1081 taaccaagca tgtaaatatc gttagacgta atgccgtcaa tcgtcatttt tgatccgcgg
1141 gagtcagtga acaggtacca tttgccgttc attttaaaga cgttcgcgcg ttcaatttca
1201 tctgttactg tgttagatgc aatcagcggt ttcatcactt ttttcagtgt gtaatcatcg
1261 tttagctcaa tcataccgag agcgccgttt gctaactcag ccgtgcgttt tttatcgctt
1321 tgcagaagtt tttgactttc ttgacggaag aatgatgtgc ttttgccata gtatgctttg
1381 ttaaataaag attcttcgcc ttggtagcca tcttcagttc cagtgtttgc ttcaaatact
1441 aagtatttgt ggcctttatc ttctacgtag tgaggatctc tcagcgtatg gttgtcgcct
1501 gagctgtagt tgccttcatc gatgaactgc tgtacatttt gatacgtttt tccgtcaccg
1561 tcaaagattg atttataatc ctctacaccg ttgatgttca aagagctgtc tgatgctgat
1621 acgttaactt gtgcagttgt cagtgtttgt ttgccgtaat gtttaccgga gaaatcagtg
1681 tagaataaac ggatttttcc gtcagatgta aatgtggctg aacctgacca ttcttgtgtt
1741 tggtctttta ggatagaatc atttgcatcg aatttgtcgc tgtctttaaa gacgcggcca
1801 gcgtttttcc agctgtcaat agaagtttcg ccgacttttt gatagaacat gtaaatcgat
1861 gtgtcatccg catttttagg atctccggct aatgcaaaga cgatgtggta gccgtgatag
1921 tttgcgacag tgccgtcagc gttttgtaat ggccagctgt cccaaacgtc caggcctttt
1981 gcagaagaga tatttttaat tgtggacgaa tcgaattcag gaacttgata tttttcattt
2041 ttttgctgtt cagggatttg cagcatatca tggcgtgtaa tatgggaaat gccgtatgtt
2101 tccttatatg gcttttggtt cgtttctttc atatgcgcaa acgcttgagt tgcgcctcct
2161 gccagcagtg cggtagtaaa ggttaatact gttgcttgtt ttgcaaactt tttgatgttc
2221 atcgttcatg tctccttttt tatgtactgt gttagcggtc tgcttcttcc agccctcctg
2281 tttgaagatg gcaagttagt tacgcacaat aaaaaaagac ctaaaatatg taaggggtga
2341 cgccaaagta tcgacctcga gtcaccgggt gacggcaacc ccgctactta cgctggccgg
2401 gcgcagtgcc cgcgacggac ggctcaagtt gtcctcgctg ccactcgctg cgacgacggg
2461 cctggcctca ccgtcccgac ctacgactca ccggtcgcga gtgccaacgt tattcttagc
2521 actcgcctat gccgagtgca agaagccccg caccgggtca tgcccctcgt tcgaccttgt
2581 cctcggccct ccgatccggg tgagtatgtt cggcccatga ccgccaacga caacaagacc
2641 cgtaaatggt cggccgcaga cgtccccgat caaagcgggc gcgtcgttgt ggtcactcga
2701 ggggggatcc cccctgcccg gttattatta tttttgacac cagaccaact ggtaatggta
2761 gcgaccggcg ctcagctgga attccgccga tactgacggg ctccaggagt cgtcgccacc
2821 aatccccata tggaaaccgt cgatattcag ccatgtgcct tcttccgcgt gcagcagatg
2881 gcgatggctg gtttccatca gttgctgttg actgtagcgg ctgatgttga actggaagtc
2941 gccgcgccac tggtgtgggc cataattcaa ttcgcgcgtc ccgcagcgca gaccgttttc
3001 gctcgggaag acgtacgggg tatacatgtc tgacaatggc agatcccagc ggtcaaaaca
3061 ggcggcagta aggcggtcgg gatagttttc ttgcggccct aatccgagcc agtttacccg
3121 ctctgctacc tgcgccagct ggcagttcag gccaatccgc gccggatgcg gtgtatcgct
3181 cgccacttca acatcaacgg taatcgccat ttgaccacta ccatcaatcc ggtaggtttt
3241 ccggctgata aataaggttt tcccctgatg ctgccacgcg tgagcggtcg taatcagcac
3301 cgcatcagca agtgtatctg ccgtgcactg caacaacgct gcttcggcct ggtaatggcc
3361 cgccgccttc cagcgttcga cccaggcgtt agggtcaatg cgggtcgctt cacttacgcc
3421 aatgtcgtta tccagcggtg cacgggtgaa ctgatcgcgc agcggcgtca gcagttgttt
3481 tttatcgcca atccacatct gtgaaagaaa gcctgactgg cggttaaatt gccaacgctt
3541 attacccagc tcgatgcaaa aatccatttc gctggtggtc agatgcggga tggcgtggga
3601 cgcggcgggg agcgtcacac tgaggttttc cgccagacgc cactgctgcc aggcgctgat
3661 gtgcccggct tctgaccatg cggtcgcgtt cggttgcact acgcgtactg tgagccagag
3721 ttgcccggcg ctctccggct gcggtagttc aggcagttca atcaactgtt taccttgtgg
3781 agcgacatcc agaggcactt caccgcttgc cagcggctta ccatccagcg ccaccatcca
3841 gtgcaggagc tcgttatcgc tatgacggaa caggtattcg ctggtcactt cgatggtttg
3901 cccggataaa cggaactgga aaaactgctg ctggtgtttt gcttccgtca gcgctggatg
3961 cggcgtgcgg tcggcaaaga ccagaccgtt catacagaac tggcgatcgt tcggcgtatc
4021 gccaaaatca ccgccgtaag ccgaccacgg gttgccgttt tcatcatatt taatcagcga
4081 ctgatccacc cagtcccaga cgaagccgcc ctgtaaacgg ggatactgac gaaacgcctg
4141 ccagtattta gcgaaaccgc caagactgtt acccatcgcg tgggcgtatt cgcaaaggat
4201 cagcgggcgc gtctctccag gtagcgaaag ccattttttg atggaccatt tcggcacagc
4261 cgggaagggc tggtcttcat ccacgcgcgc gtacatcggg caaataatat cggtggccgt
4321 ggtgtcggct ccgccgcctt catactgcac cgggcgggaa ggatcgacag atttgatcca
4381 gcgatacagc gcgtcgtgat tagcgccgtg gcctgattca ttccccagcg accagatgat
4441 cacactcggg tgattacgat cgcgctgcac cattcgcgtt acgcgttcgc tcatcgccgg
4501 tagccagcgc ggatcatcgg tcagacgatt cattggcacc atgccgtggg tttcaatatt
4561 ggcttcatcc accacataca ggccgtagcg gtcgcacagc gtgtaccaca gcggatggtt
4621 cggataatgc gaacagcgca cggcgttaaa gttgttctgc ttcatcagca ggatatcctg
4681 caccatcgtc tgctcatcca tgacctgacc atgcagagga tgatgctcgt gacggttaac
4741 gcctcgaatc agcaacggct tgccgttcag cagcagcaga ccattttcaa tccgcacctc
4801 gcggaaaccg acatcgcagg cttctgcttc aatcagcgtg ccgtcggcgg tgtgcagttc
4861 aaccaccgca cgatagagat tcgggatttc ggcgctccac agtttcgggt tttcgacgtt
4921 cagacgtagt gtgacgcgat cggcataacc accacgctca tcgataattt caccgccgaa
4981 aggcgcggtg ccgctggcga cctgcgtttc accctgccat aaagaaactg ttacccgtag
5041 gtagtcacgc aactcgccgc acatctgaac ttcagcctcc agtacagcgc ggctgaaatc
5101 atcattaaag cgagtggcaa catggaaatc gctgatttgt gtagtcggtt tatgcagcaa
5161 cgagacgtca cggaaaatgc cgctcatccg ccacatatcc tgatcttcca gataactgcc
5221 gtcactccaa cgcagcacca tcaccgcgag gcggttttct ccggcgcgta aaaatgcgct
5281 caggtcaaat tcagacggca aacgactgtc ctggccgtaa ccgacccagc gcccgttgca
5341 ccacagatga aacgccgagt taacgccatc aaaaataatt cgcgtctggc cttcctgtag
5401 ccagctttca tcaacattaa atgtgagcga gtaacaaccc gtcggattct ccgtgggaac
5461 aaacggcgga ttgaccgtaa tgggataggt tacgttggtg tagatgggcg catcgtaacc
5521 gtgcatctgc cagtttgagg ggacgacgac agtatcggcc tcaggaagat cgcactccag
5581 ccagctttcc ggcaccgctt ctggtgccgg aaaccaggca aagcgccatt cgccattcag
5641 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
5701 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
5761 acgttgtaaa acgacgggat ccattcttgc ttccctcatc ctcatctcaa cgcatccatg
5821 catgtttggg cgcatcctga attaggtcag actgcaggcg ctgggcccgg cagtgctcgt
5881 gtagtcaacc acaacttcgg gcgtccaccc gcatcaagcg caccgccgaa acccttatcc
5941 ggcggtcgtt cacggccaat tcgggaccga cgcgacggcc tgaaggtggc atttccgcag
6001 tgtctgggca tgtgtcgtct agagcggccg ccaccgcggt ggagctcagc cagatcctat
6061 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
6121 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
6181 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
6241 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
6301 tcgctagaaa tcatccttag cgaaagctaa ggattttttt tatctgaatt ggtaccgcgg
6361 ccgccggggg ccgggggcgg cgccgggcgg cccggggcgt caggcgccgg gggcggtgtc
6421 cggcggcccc cagaggaact gcgccagttc ctccggatcg gtgaagccgg agagatccag
6481 cggggtctcc tcgaacacct cgaagtcgtg caggaaggtg aaggcgagca gttcgcgggc
6541 gaagtcctcg gtccgcttcc actgcgcccc gtcgagcagc gcggccagga tctcgcggtc
6601 gccccggaag gcgttgagat gcagttgcac caggctgtag cgggagtctc ccgcatagac
6661 gtcggtgaag tcgacgatcc cggtgacctc ggtcgcggcc aggtccacga agatgttggt
6721 cccgtgcagg tcgccgtgga cgaaccgggg ttcgcggccg gccagcagcg tgtccacgtc
6781 cggcagccag tcctccaggc ggtccagcag ccggggcgag aggtagcccc acccgcggtg
6841 gtcctcgacg gtcgccgcgc ggcgttcccg cagcagttcc gggaagacct cggaatgggg
6901 ggtgagcacg gtgttcccgg tcagcggcac cctgtgcagc cggccgagca cccggccgag
6961 ttcgcgggcc agggcgagca gcgcgttccg gtcggtcgtg ccgtccatcg cggaccgcca
7021 ggtggtgccg gtcatccggc tcatcaccag gtagggccac ggccaggctc cggtgccggg
7081 ccgcagctcg ccgcggccga ggaggcgggg caccggcacc ggggcgtccg ccaggaccgc
7141 gtacgcctcc gactccgacg cgaggctctc cggaccgcac cagtgctcgc cgaacagctt
7201 gatcaccggg tcgggctcgc cgaccagtac ggggttggtg ctctcgccgg gcacccgcag
7261 caccggcggc accggcagcc cgagctcctc cagggctcgg cgggccagcg gctcccagaa
7321 ttcctggtcg ttccgcaggc tcgcgtagga atcatccgaa tcaatacggt cgagaagtaa
7381 cagggattct tgtgtcacag cggacctcta ttcacagggt acgggccggc ttaattccgc
7441 acggccggtc gcgacacggc ctgtccgcac cgcggtcagg cgttgacgat gacgggctgg
7501 tcggccacgt cggggacgtt ctcggtggtg ctgcggtcgg gatcgccaat ctctacgggc
7561 cgaccgaggc gacggtgtac gccaccgcct ggttctgcga cggcgaggcg ccgccaggcc
7621 ccgccgatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
7861 nnnnnnnnnn nnnnnnnnnn nnctgcaggc atgcnnnnnn agatccatgg atatctagat
7921 ttaaagatct ggtaccgcgg ccgcttaatt aagaattctc atgtttgaca gcttatcatc
7981 gataagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt
8041 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc
8101 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atctgcctcg cgcgtttcgg
8161 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
8221 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
8281 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
8341 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
8401 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
8461 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
8521 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
8581 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
8641 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
8701 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
8761 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
8821 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
8881 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
8941 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
9001 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
9061 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
9121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
9181 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
9241 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
9301 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
9361 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
9421 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
9481 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
9541 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
9601 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
9661 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
9721 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
9781 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
9841 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
9901 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
9961 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
10021 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
10081 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
10141 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
10201 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
10261 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
10321 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
10381 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaag
//
