| 出品人: | Lynch,M.D. and Gill,R.T. |
|---|---|
| 载体名称: | pBTL-4 |
| 质粒类型: | 广宿主载体；克隆载体；原核表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 2996 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 四环素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pBTL-4载体质粒基本信息
pBTL-4质粒图谱载体图谱和pBTL-4载体序列质粒序列多克隆位点信息
pBTL-4质粒载体简介
pBTL-4质粒序列载体序列
LOCUS DQ058742 2996 bp DNA circular SYN 08-MAY-2006
DEFINITION Broad host range vector pBTL-4, complete sequence.
ACCESSION DQ058742
VERSION DQ058742.1 GI:69048475
AUTHORS Lynch,M.D. and Gill,R.T.
TITLE Broad host range vectors for stable genomic library construction
JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering,
University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
80309, USA
FEATURES Location/Qualifiers
source 1..2996
/organism="Broad host range vector pBTL-4"
/mol_type="other DNA"
/db_xref="taxon:334974"
regulatory 60..96
/regulatory_class="terminator"
/note="SoxR terminator"
gene 872..1534
/gene="rep"
/note="rep gene from pBBR1, GenBank Accession Number
X66730"
CDS 872..1534
/gene="rep"
/note="required for replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AAY99753.1"
/db_xref="GI:69048476"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
gene 1631..2821
/gene="tetA"
/note="tetracycline resistance gene from pBR322"
CDS 1631..2821
/gene="tetA"
/codon_start=1
/transl_table=11
/product="tetracycline/H+ antiporter"
/protein_id="AAY99754.1"
/db_xref="GI:69048477"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
regulatory 2841..2872
/regulatory_class="terminator"
/note="TonB terminator"
regulatory 2899..2981
/regulatory_class="promoter"
/note="lac promoter"
ORIGIN
1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
1621 ggaagctaaa atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga
1681 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca
1741 ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt
1801 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct
1861 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg
1921 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
1981 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
2041 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
2101 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
2161 ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
2221 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
2281 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
2341 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
2401 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
2461 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
2521 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
2581 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
2641 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
2701 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
2761 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
2821 aatggaaggg atccccctca agtcaaaagc ctccggtcgg aggcttttga ctttctgcta
2881 tggaggtcag gtatgatttt gcattaggca ccccaggctt tacactttat gcttccggct
2941 cgtatgttgt gtggaattgt gagcggataa caatttcaca ctctagagaa ttcgtc
//
