Rosetta-gami(DE3)pLysS

价格：800元

联系方式：I47-825O-882O

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感受态细胞基本信息

出品公司:	Novagen/ Millipore
感受态细胞名称:	Rosetta-gami(DE3)pLysS, Rosetta gami DE3 pLysS
菌株类型:	E.coli
产品目录号:	71057-3, 71057-4
培养基:	LB培养基
生长条件:	37 ℃, 有氧
基因型:
抗性:	氯霉素，卡那，链霉素，四环素
质粒转化条件:	42 ℃热激
应用:	蛋白表达
诱导方法:	IPTG
菌株特点:	Rosetta-gami系列菌株来源于Origami系列宿主菌，该系列菌株的有点除了含有Origami菌株原有的TrxB和Gor基因突变提高含二硫键正确折叠蛋白的高效表达，同时含有Rosetta系列菌株中含有的真核细胞稀有密码子。该菌株同时集合了Origami(DE3)和Rosetta(DE3)的优点。 Rosetta-gami(DE3)pLysS系列菌株含有pRARE质粒，能够提供AUA, AGG, AGA, CUA, CCC, 和GGA六个真核细胞中的稀有密码子tRNA，能够有效提升真核细胞蛋白的表达水平。pRARE质粒具有氯霉素抗性。 Rosetta-gami(DE3)pLysS感受态细胞同时具有氯霉素，卡那，链霉素和四环素四种抗生素抗性。 Rosetta-gami(DE3)pLysS被建议使用于含有Bla标记的氨苄抗性表达质粒。当使用pCDF系列载体时，还需要使用壮观霉素(Spectinomycin)进行筛选。菌株中的rpsL突变使得该菌株具有链霉素抗性 DE3是溶源性的 λDE3, 所以带有T7 RNA聚合酶的染色体拷贝。该菌株适用于pET系列载体，及其他T7启动子系列载体。含有pLSs质粒，pLySs质粒能够产生T7溶菌酶，可以有效降低目的基因的基础表达水平。pLySs质粒使得该菌株具有氯霉素抗性。pLySs质粒的起始复制位点是p15 Origin, 这使得该质粒能够和pUC-及pBR322-衍生的质粒互相共存。亮氨酸生长缺陷型菌株。

JM109(DE3) Chemically Competent Cell	C58C1 Chemically Competent Cell
EGY48 Chemically Competent Cell	LBA4404 Chemically Competent Cell
BL21 Star(DE3)pLysS Chemically Competent	XL10-Gold Chemically Competent Cell
Mach1-T1 Chemically Competent Cell	K599 Chemically Competent Cell
Ar 1193 Chemically Competent Cell	MSU440 Chemically Competent Cell
Ar.Qual Chemically Competent Cell	Ar.A4 Chemically Competent Cell
AGL1(pSoup) Chemically Competent Cell	EHA105(pSoup) Chemically Competent Cell
GV3101 (pJIC SA_Rep) Chemically Competen	GV3101(pSoup-p19) Chemically Competent C
GV3101(pSoup) Chemically Competent Cell	EHA101 Chemically Competent Cell
AGL1 Chemically Competent Cell	YM4271 Chemically Competent Cell
R5421 Chemically Competent Cell	Y190 Chemically Competent Cell
BY4741 Chemically Competent Cell	INVSc1 Chemically Competent Cell
NMY51 Chemically Competent Cell	AH109 Chemically Competent Cell
Y187 Chemically Competent Cell	Y2HGold Chemically Competent Cell
Y1HGold Chemically Competent Cell	SHuffle T7 E. coli Chemically Competent
ArcticExpress (DE3) pRARE2 Chemically Co	ArcticExpress (DE3) Chemically Competent
HT115(DE3) Chemically Competent Cell	M15(pREP4) Chemically Competent Cell
ER2566 Chemically Competent Cell	C43(DE3) pLysS Chemically Competent Cell
OverExpress C43(DE3) Chemically Competen	TB1 Chemically Competent Cell
OrigamiB(DE3)pLysS Chemically Competent	Rosetta-gami B(DE3) Chemically Competent
Rosetta 2(DE3) Chemically Competent Cell	Rosetta(DE3) Chemically Competent Cell
BL21-CodonPlus (DE3)-RIPL Chemically Com	BL21(AI) Chemically Competent Cell
BW25113 Chemically Competent Cell	JM108 Chemically Competent Cell
MG1655 Chemically Competent Cell	JM330 Chemically Competent Cell
DH5α λpir Chemically Competent Cell	S17-1 λpir Chemically Competent Cell
MC1061F- Chemically Competent Cell	GT115 Chemically Competent Cell
EPI300 Chemically Competent Cell	EPI400 Chemically Competent Cell
TOP10F` Chemically Competent Cell	Turbo Chemically Competent Cell
Stable Chemically Competent Cell	BJ5183-AD-1 Chemically Competent Cell
HB101 Chemically Competent Cell	TG1 Chemically Competent Cell
DB3.1 Chemically Competent Cell	XL2-Blue Chemically Competent Cell
XL1-Blue MRF` Kan Chemically Competent C	JM110 Chemically Competent Cell
TOP10	DH5α
Origami 2(DE3)	DH10Bac
Stbl4感受态细胞	Stbl3 Ultracompetent Cells
Origami 2	Origami 2(DE3)pLysS
Origami 2(DE3)pLacI	Origami B
OrigamiB(DE3)pLysS	OrigamiB(DE3)pLacI
GV3101	BL21(DE3)
Origami(DE3)	OrigamiB(DE3)
Origami	Origami(DE3)pLysS
Origami(DE3)pLacI	BL21 Star(DE3)
BL21 Star(DE3)pLySs	BL21-AI
Sure	M15[pREP4]
One Shot ccdB Survival 2 T1R Competent C	BL21-Gold(DE3)
Rosetta(DE3)	Rosetta(DE3)pLySs
Rosetta-gami(DE3)	BL21(DE3)pLySs
RosettaBlue(DE3)	BL21
Rosetta-gami 2(DE3)	Rosetta-gami(DE3)pLysS
Rosetta-gami 2(DE3)pLysS	Rosetta-gami B(DE3)

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Rosetta-gami(DE3)pLysS

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