Rosetta-gami2(DE3)pLacI

价格：1000元

联系方式：I47-825O-882O

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感受态细胞基本信息

出品公司:	Novagen/ Millipore
感受态细胞名称:	Rosetta-gami 2(DE3)pLacI, Rosetta-gami2(DE3)pLacI, Rosetta gami 2 DE3 pLacI, Rosetta-gami2 DE3 pLacI
菌株类型:	E.coli
产品目录号:	71353-3, 71353-4
培养基:	LB培养基
生长条件:	37 ℃, 有氧
基因型:
抗性:	氯霉素，链霉素，四环素
质粒转化条件:	42 ℃热激
应用:	蛋白表达
诱导方法:	IPTG
菌株特点:	Rosetta-gami 2系列菌株是集合了Rosetta 2系列菌株和Origami 2系列菌株的有点，增强了细胞内二硫键的形成和含有在大肠杆菌中稀有密码子的真核细胞蛋白的表达能力。 Rosetta-gami 2(DE3)系列菌株是来源于Origami 2系列菌株。而Origami 2 系列菌株是卡那敏感的K-12细胞菌株，携带有TrxB和Gor基因突变提高含二硫键正确折叠蛋白的高效表达。本菌株含有pRARE2质粒，除了能够提供原Rosetta(DE3)宿主菌含有的AUA, AGG, AGA, CUA, CCC, 和GGA六个稀有密码子的tRNA外，还提供了第七个稀有密码子CGG的tRNA。同时，pRARE2质粒具有氯霉素抗性。通过提供原本在大肠杆菌中稀少的真核细胞密码子，增加了真核细胞的在大肠杆菌中的蛋白表达水平。 Rosetta-gami 2(DE3)感受态细胞中的Gor基因突变使得菌株具有四环素抗性。 DE3是溶源性的 λDE3, 所以带有T7 RNA聚合酶的染色体拷贝。该菌株适用于pET系列载体，及其他T7启动子系列载体。 pLacI基因型表明该菌株含有pACYC184质粒，该质粒含有Lac抑制子基因。亮氨酸生长缺陷型菌株。

JM109(DE3) Chemically Competent Cell	C58C1 Chemically Competent Cell
EGY48 Chemically Competent Cell	LBA4404 Chemically Competent Cell
BL21 Star(DE3)pLysS Chemically Competent	XL10-Gold Chemically Competent Cell
Mach1-T1 Chemically Competent Cell	K599 Chemically Competent Cell
Ar 1193 Chemically Competent Cell	MSU440 Chemically Competent Cell
Ar.Qual Chemically Competent Cell	Ar.A4 Chemically Competent Cell
AGL1(pSoup) Chemically Competent Cell	EHA105(pSoup) Chemically Competent Cell
GV3101 (pJIC SA_Rep) Chemically Competen	GV3101(pSoup-p19) Chemically Competent C
GV3101(pSoup) Chemically Competent Cell	EHA101 Chemically Competent Cell
AGL1 Chemically Competent Cell	YM4271 Chemically Competent Cell
R5421 Chemically Competent Cell	Y190 Chemically Competent Cell
BY4741 Chemically Competent Cell	INVSc1 Chemically Competent Cell
NMY51 Chemically Competent Cell	AH109 Chemically Competent Cell
Y187 Chemically Competent Cell	Y2HGold Chemically Competent Cell
Y1HGold Chemically Competent Cell	SHuffle T7 E. coli Chemically Competent
ArcticExpress (DE3) pRARE2 Chemically Co	ArcticExpress (DE3) Chemically Competent
HT115(DE3) Chemically Competent Cell	M15(pREP4) Chemically Competent Cell
ER2566 Chemically Competent Cell	C43(DE3) pLysS Chemically Competent Cell
OverExpress C43(DE3) Chemically Competen	TB1 Chemically Competent Cell
OrigamiB(DE3)pLysS Chemically Competent	Rosetta-gami B(DE3) Chemically Competent
Rosetta 2(DE3) Chemically Competent Cell	Rosetta(DE3) Chemically Competent Cell
BL21-CodonPlus (DE3)-RIPL Chemically Com	BL21(AI) Chemically Competent Cell
BW25113 Chemically Competent Cell	JM108 Chemically Competent Cell
MG1655 Chemically Competent Cell	JM330 Chemically Competent Cell
DH5α λpir Chemically Competent Cell	S17-1 λpir Chemically Competent Cell
MC1061F- Chemically Competent Cell	GT115 Chemically Competent Cell
EPI300 Chemically Competent Cell	EPI400 Chemically Competent Cell
TOP10F` Chemically Competent Cell	Turbo Chemically Competent Cell
Stable Chemically Competent Cell	BJ5183-AD-1 Chemically Competent Cell
HB101 Chemically Competent Cell	TG1 Chemically Competent Cell
DB3.1 Chemically Competent Cell	XL2-Blue Chemically Competent Cell
XL1-Blue MRF` Kan Chemically Competent C	JM110 Chemically Competent Cell
TOP10	DH5α
Origami 2(DE3)	DH10Bac
Stbl4感受态细胞	Stbl3 Ultracompetent Cells
Origami 2	Origami 2(DE3)pLysS
Origami 2(DE3)pLacI	Origami B
OrigamiB(DE3)pLysS	OrigamiB(DE3)pLacI
GV3101	BL21(DE3)
Origami(DE3)	OrigamiB(DE3)
Origami	Origami(DE3)pLysS
Origami(DE3)pLacI	BL21 Star(DE3)
BL21 Star(DE3)pLySs	BL21-AI
Sure	M15[pREP4]
One Shot ccdB Survival 2 T1R Competent C	BL21-Gold(DE3)
Rosetta(DE3)	Rosetta(DE3)pLySs
Rosetta-gami(DE3)	BL21(DE3)pLySs
RosettaBlue(DE3)	BL21
Rosetta-gami 2(DE3)	Rosetta-gami(DE3)pLysS
Rosetta-gami 2(DE3)pLysS	Rosetta-gami B(DE3)

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Rosetta-gami2(DE3)pLacI

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