TKB1

价格：1200元

联系方式：I47-825O-882O

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感受态细胞基本信息

出品公司:	Agilent
感受态细胞名称:	BL21感受态细胞, BL 21, BL-21
菌株类型:	E.coli
产品目录号:	200134
培养基:	LB培养基
生长条件:	37 ℃, 有氧
基因型:
抗性:	四环素
质粒转化条件:	42 ℃热激
应用:	毒性蛋白表达
诱导方法:	IPTG
菌株特点:	作为E.coli B宿主菌，主要用来进行蛋白表达，细胞内缺少lon蛋白酶和ompT外膜蛋白酶，能够有效避免目的蛋白的降解。 TKB1感受态细胞是BL21(DE3)衍生而来，含有酪氨酸激酶(Tyrosine Kinase)。BL21(DE3)TK感受态细胞，简写为TKB1，带有T7 RNA聚合酶的染色体拷贝，该基因的启动子是LacUV5。通过IPTG诱导表达的T7 RNA聚合酶，允许其下游目的蛋白表达，从而实现对下游目的蛋白的可控诱导型基因表达。 TKB1感受态细胞内含有一个能有诱导表达酪氨酸激酶基因的质粒（pTK）。 TKB1感受态细胞能够转化进去ColE1 Origin为复制起点的，包含有编码磷酸化目的基团或者蛋白的质粒。如果目的蛋白是构建在一个含有亲和标签的融合表达载体上面，那么对于磷酸化蛋白的纯化就会相对比较容易。从TKB1感受态细胞中纯化得到的目的蛋白，能够用来筛选表达库，或者亲和纯化和蛋白印记酪氨酸磷酸化蛋白。

JM109(DE3) Chemically Competent Cell	C58C1 Chemically Competent Cell
EGY48 Chemically Competent Cell	LBA4404 Chemically Competent Cell
BL21 Star(DE3)pLysS Chemically Competent	XL10-Gold Chemically Competent Cell
Mach1-T1 Chemically Competent Cell	K599 Chemically Competent Cell
Ar 1193 Chemically Competent Cell	MSU440 Chemically Competent Cell
Ar.Qual Chemically Competent Cell	Ar.A4 Chemically Competent Cell
AGL1(pSoup) Chemically Competent Cell	EHA105(pSoup) Chemically Competent Cell
GV3101 (pJIC SA_Rep) Chemically Competen	GV3101(pSoup-p19) Chemically Competent C
GV3101(pSoup) Chemically Competent Cell	EHA101 Chemically Competent Cell
AGL1 Chemically Competent Cell	YM4271 Chemically Competent Cell
R5421 Chemically Competent Cell	Y190 Chemically Competent Cell
BY4741 Chemically Competent Cell	INVSc1 Chemically Competent Cell
NMY51 Chemically Competent Cell	AH109 Chemically Competent Cell
Y187 Chemically Competent Cell	Y2HGold Chemically Competent Cell
Y1HGold Chemically Competent Cell	SHuffle T7 E. coli Chemically Competent
ArcticExpress (DE3) pRARE2 Chemically Co	ArcticExpress (DE3) Chemically Competent
HT115(DE3) Chemically Competent Cell	M15(pREP4) Chemically Competent Cell
ER2566 Chemically Competent Cell	C43(DE3) pLysS Chemically Competent Cell
OverExpress C43(DE3) Chemically Competen	TB1 Chemically Competent Cell
OrigamiB(DE3)pLysS Chemically Competent	Rosetta-gami B(DE3) Chemically Competent
Rosetta 2(DE3) Chemically Competent Cell	Rosetta(DE3) Chemically Competent Cell
BL21-CodonPlus (DE3)-RIPL Chemically Com	BL21(AI) Chemically Competent Cell
BW25113 Chemically Competent Cell	JM108 Chemically Competent Cell
MG1655 Chemically Competent Cell	JM330 Chemically Competent Cell
DH5α λpir Chemically Competent Cell	S17-1 λpir Chemically Competent Cell
MC1061F- Chemically Competent Cell	GT115 Chemically Competent Cell
EPI300 Chemically Competent Cell	EPI400 Chemically Competent Cell
TOP10F` Chemically Competent Cell	Turbo Chemically Competent Cell
Stable Chemically Competent Cell	BJ5183-AD-1 Chemically Competent Cell
HB101 Chemically Competent Cell	TG1 Chemically Competent Cell
DB3.1 Chemically Competent Cell	XL2-Blue Chemically Competent Cell
XL1-Blue MRF` Kan Chemically Competent C	JM110 Chemically Competent Cell
TOP10	DH5α
Origami 2(DE3)	DH10Bac
Stbl4感受态细胞	Stbl3 Ultracompetent Cells
Origami 2	Origami 2(DE3)pLysS
Origami 2(DE3)pLacI	Origami B
OrigamiB(DE3)pLysS	OrigamiB(DE3)pLacI
GV3101	BL21(DE3)
Origami(DE3)	OrigamiB(DE3)
Origami	Origami(DE3)pLysS
Origami(DE3)pLacI	BL21 Star(DE3)
BL21 Star(DE3)pLySs	BL21-AI
Sure	M15[pREP4]
One Shot ccdB Survival 2 T1R Competent C	BL21-Gold(DE3)
Rosetta(DE3)	Rosetta(DE3)pLySs
Rosetta-gami(DE3)	BL21(DE3)pLySs
RosettaBlue(DE3)	BL21
Rosetta-gami 2(DE3)	Rosetta-gami(DE3)pLysS
Rosetta-gami 2(DE3)pLysS	Rosetta-gami B(DE3)

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TKB1

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感受态细胞基本信息

感受态细胞特点和简介

感受态细胞制备方法

感受态细胞操作方法

感受态细胞注意事项

感受态细胞说明书pdf版和相关资料下载

感受态细胞应用举例

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