| 质粒名称: | pTM1_VP30_ZEBOV |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 69119 |
| 存储实验室: | Elke Mühlberger |
|
载体骨架基本信息 |
|
| 载体名称: | pTM1 |
| 载体出品公司: | Bernhard Moss, National Institutes of Health |
| 空载体大小: | 5357 bp |
| 完整质粒大小: | 7136 bp |
| 载体类型: | T7 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | VP30 ZEBOV |
| 物种来源: | 光合细菌 Rhodopseudomonas palustris |
| 插入大小: | 866 bp |
| 启动子: | T7 |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (not destroyed) |
| 3’克隆位点: | PstI (not destroyed) |
| 5’测序引物 | ATT GTA TGG GAT CTG ATC TGG |
| 3’测序引物 | GCC AAC TCA GCT TCC TTT CGG |
|
文献引用方法 |
|
| 材料和方法部分: | pTM1_VP30_ZEBOV was a gift from Elke Mühlberger (Addgene plasmid # 69119 ; http://n2t.net/addgene:69119 ; RRID:Addgene_69119) |
| 参考文献部分: | The L-VP35 and L-L interaction domains reside in the amino terminus of the Ebola virus L protein and are potential targets for antivirals. Trunschke M, Conrad D, Enterlein S, Olejnik J, Brauburger K, Muhlberger E. Virology. 2013 Jul 5;441(2):135-45. doi: 10.1016/j.virol.2013.03.013. Epub 2013 Apr 11. 10.1016/j.virol.2013.03.013 PubMed 23582637 |
买家导航
pTM1_VP30_ZEBOV载体质粒基本信息
pTM1_VP30_ZEBOV质粒图谱载体图谱和pTM1_VP30_ZEBOV载体序列质粒序列多克隆位点信息
pTM1_VP30_ZEBOV质粒载体简介
pTM1_VP30_ZEBOV质粒序列载体序列
LOCUS pTM1_VP30_ZEBOV 7138 bp DNA circular SYN 25-AUG-2024
DEFINITION Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA
polymerase promoter.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7138)
AUTHORS Trunschke M, Conrad D, Enterlein S, Olejnik J, Brauburger K,
Muhlberger E
TITLE The L-VP35 and L-L interaction domains reside in the amino terminus
of the Ebola virus L protein and are potential targets for
antivirals.
JOURNAL Virology. 2013 Jul 5;441(2):135-45. doi:
10.1016/j.virol.2013.03.013. Epub 2013 Apr 11.
PUBMED 23582637
REFERENCE 2 (bases 1 to 7138)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.virol.2013.03.013"; journalName: "Virology"; date:
"2013-07-5- 5"; volume: "441"; issue: "2"; pages: "135-45"
FEATURES Location/Qualifiers
source 1..7138
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 794..813
/label=T7
/note="T7 promoter, forward primer"
promoter 794..812
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 851..1423
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1018..1035)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 1245..1264
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
primer_bind complement(3323..3341)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 3337..3384
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind complement(4364..4386)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 4516..4971
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4603..4622)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4813..4834
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 5000..5104
/gene="bla"
/label=AmpR promoter
CDS 5105..5965
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5323..5342)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6136..6724
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6625..6644
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6878..6895
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 7012..7033
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7048..7078
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7086..7102
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7091..7113
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 7110..7126
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 7110..7126
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
ORIGIN
1 aagcttttgc gatcaataaa tggatcacaa ccagtatctc ttaacgatgt tcttcgcaga
61 tgatgattca ttttttaagt atttggctag tcaagatgat gaatcttcat tatctgatat
121 attgcaaatc actcaatatc tagactttct gttattatta ttgatccaat caaaaaataa
181 attagaagcc gtgggtcatt gttatgaatc tctttcagag gaatacagac aattgacaaa
241 attcacagac tttcaagatt ttaaaaaact gtttaacaag gtccctattg ttacagatgg
301 aagggtcaaa cttaataaag gatatttgtt cgactttgtg attagtttga tgcgattcaa
361 aaaagaatcc tctctagcta ccaccgcaat agatcctgtt agatacatag atcctcgtcg
421 caatatcgca ttttctaacg tgatggatat attaaagtcg aataaagtga acaataatta
481 attctttatt gtcatcatga acggcggaca tattcagttg ataatcggcc ccatgttttc
541 aggtaaaagt acagaattaa ttagacgagt tagacgttat caaatagctc aatataaatg
601 cgtgactata aaatattcta acgataatag atacggaacg ggactatgga cgcatgataa
661 gaataatttt gaagcattgg aagcaactaa actatgtgat gtcttggaat caattacaga
721 tttctccgtg ataggtatcg atgaaggaca gttctttcca gacattgttg aattgatctc
781 gatcccgcga aattaatacg actcactata gggagaccac aacggtttcc ctctagcggg
841 atcaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
901 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
961 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
1021 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
1081 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
1141 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
1201 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
1261 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
1321 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
1381 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggg aattccccgg
1441 ggagctcact agtggatccg cgaaccggta gagtttagtt gcaacctaac acacataaag
1501 cattggtcaa aaagtcaata gaaatttaaa cagtgagtgg agacaacttt taaatggaag
1561 cttcatatga gagaggacgc ccacgagctg ccagacagca ttcaagggat ggacacgacc
1621 accatgttcg agcacgatca tcatccagag agaattatcg aggtgagtac cgtcaatcaa
1681 ggagcgcctc acaagtgcgc gttcctactg tatttcataa gaagagagtt gaaccattaa
1741 cagttcctcc agcacctaaa gacatatgtc cgaccttgaa aaaaggattt ttgtgtgaca
1801 gtagtttttg caaaaaagat caccagttgg agagtttaac tgatagggaa ttactcctac
1861 taatcgcccg taagacttgt ggatcagtag aacaacaatt aaatataact gcacccaagg
1921 actcgcgctt agcaaatcca acggctgatg atttccagca agaggaaggt ccaaaaatta
1981 ccttgttgac actgatcaag acggcagaac actgggcgag acaagacatc agaaccatag
2041 aggattcaaa attaagagca ttgttgactc tatgtgctgt gatgacgagg aaattctcaa
2101 aatcccagct gagtctttta tgtgagacac acctaaggcg cgaggggctt gggcaagatc
2161 aggcagaacc cgttctcgaa gtatatcaac gattacacag tgataaagga ggcagttttg
2221 aagctgcact atggcaacaa tgggaccgac aatccctaat tatgtttatc actgcattct
2281 tgaatattgc tctccagtta ccgtgtgaaa gttctgctgt cgttgtttca gggttaagaa
2341 cattggttcc tcaatcagat aatgaggaag cttcaaccaa cccggggaca tgctcatggt
2401 ctgatgaggg taccccttaa taaggctgac taaaacacta tataaccttc tacttgatca
2461 caatactccg tatacctatc atcatatatt taatcaagac gatatccttt aaaacttatt
2521 cagtactata atcactctcg tttcaaatta ataagatgtg catgattgcc ctaatatatg
2581 aagaggtatg atacaaccct aacagtgatc aaagaaaatc ataatctcgt atcgctcgaa
2641 atataacctg ccaagcatac ctcttgcaca aagtgattct tgtacacaaa taatgtttta
2701 ctctacagga ggtagcaacg atccatccca tcaaaaaata agtatttcat gacttactaa
2761 tgatctctta aaatattaag aaaaactgac ggaacataaa ttctttatgc ttcaagctgt
2821 ggaggaggtg tttggtattg gctattgtta tattacaatc aataacaagc ttgtaaaaat
2881 attgttcttg tttcaagagg tagattgtga ccggaaatgc taaactaatg atgaagatta
2941 atgcggaggt ctgataagaa taaaccttat tattcagatt aggccccaag aggcattctt
3001 catctccttt tagcaaagta ctatttcagg gtagtccaat tagtggcacg tcttttagct
3061 gtatatcagt cgcccctgag atacgccaca aaagtgtctc taagctaaat tggtctgtac
3121 acatcccata cattgtatta ggggcaataa tatctaattg aacttagccg tttaaaattt
3181 agtgcataaa tctgggctaa caccaccagg tcaactccat tggctgaaaa gaagcttacc
3241 tgcagctcga gaggcctaat taattaagtc gacgatccgg ctgctaacaa agcccgaaag
3301 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
3361 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc gagatcaatt
3421 ctgtgagcgt atggcaaacg aaggaaaaat agttatagta gccgcactcg atgggacatt
3481 tcaacgtaaa ccgtttaata atattttgaa tcttattcca ttatctgaaa tggtggtaaa
3541 actaactgct gtgtgtatga aatgctttaa ggaggcttcc ttttctaaac gattgggtga
3601 ggaaaccgag atagaaataa taggaggtaa tgatatgtat caatcggtgt gtagaaagtg
3661 ttacatcgac tcataatatt atatttttta tctaaaaaac taaaaataaa cattgattaa
3721 attttaatat aatacttaaa aatggatgtt gtgtcgttag ataaaccgtt tatgtatttt
3781 gaggaaattg ataatgagtt agattacgaa ccagaaagtg caaatgaggt cgcaaaaaaa
3841 ctgccgtatc aaggacagtt aaaactatta ctaggagaat tattttttct tagtaagtta
3901 cagcgacacg gtatattaga tggtgccacc gtagtgtata taggatctgc tcccggtaca
3961 catatacgtt atttgagaga tcatttctat aatttaggag tgatcatcaa atggatgcta
4021 attgacggcc gccatcatga tcctatttta aatggattgc gtgatgtgac tctagtgact
4081 cggttcgttg atgaggaata tctacgatcc atcaaaaaac aactgcatcc ttctaagatt
4141 attttaattt ctgatgtgag atccaaacga ggaggaaatg aacctagtac ggcggattta
4201 ctaagtaatt acgctctaca aaatgtcatg attagtattt taaaccccgt ggcgtctagt
4261 cttaaatgga gatgcccgtt tccagatcaa tggatcaagg acttttatat cccacacggt
4321 aataaaatgt tacaaccttt tgctccttca tattcagggc cgtcgtttta caacgtcgtg
4381 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
4441 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
4501 atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
4561 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
4621 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
4681 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
4741 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
4801 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
4861 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
4921 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcccaggtgg
4981 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
5041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
5101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
5161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
5221 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
5281 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
5341 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
5401 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
5461 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
5521 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
5581 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
5641 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
5701 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
5761 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
5821 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
5881 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
5941 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
6001 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
6061 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
6121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
6181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
6241 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
6301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
6361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
6421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
6481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
6541 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
6601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
6661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
6721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
6781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
6841 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
6901 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
6961 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
7021 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
7081 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgcc
//
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