pAc5/V5-HisA

价格：1600元

联系方式：I47-825O-882O

买家导航

pAc5/V5-HisA载体质粒基本信息

出品公司:	Invitrogen
载体名称:	pAc5/V5-HisA 、pAc5/V5-His A
质粒类型:	昆虫表达载体；果蝇细胞表达载体
克隆方法:	多克隆位点，限制性内切酶
启动子:	P-AC5 (Actin 5C)
载体大小:	5.4 kb
5' 测序引物及序列:	AC5 Forward：5'd[ACACAAAGCCGCTCCATCAG]3'
3' 测序引物及序列:	--
载体标签:	V5 Epitope Tag , His Tag (C-末端)
载体抗性:	氨苄青霉素
真核筛选标记:	无
克隆菌株:	TOP10；DH5-T1R
宿主细胞（系）:	果蝇细胞系S2
备注:	pAc5-V5-His 载体可以与筛选载体pCoHygro或者pCoBlast共转染，建立稳表达细胞系。
产品目录号:	V4110-20
瞬表达/稳表达:	瞬表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

pAc5/V5-HisA质粒图谱载体图谱和pAc5/V5-HisA载体序列质粒序列多克隆位点信息

pAc5-V5-HisA载体图谱

pAc5/V5-HisA质粒载体简介

pAc5/V5-HisA质粒序列载体序列

CTTCAAGGAATTAATTCTATATTCTAAAAACACAAATGATACTTCTAAAAAAAATCATGAATGGCATCAACT
CTGAATCAAATCTTTGCAGATGCACCTACTTCTCATTTCCACTGTCACATCATTTTTCCAGATCTCGCTGCC
TGTTATGTGGCCCACAAACCAAGACACGTTTTATGGCCATTAAAGCTGGCTGATCGTCGCCAAACACCAAAT
ACATATCAATATGTACATTCGAGAAAGAAGCGATCAAAGAAGCGTCTTCGGGCGAGTAGGAGAATGCGGAGG
AGAAGGAGAACGAGCTGATCTAGTATCTCTCCACAATCCAATGCCAACTGACCAACTGGCCATATTCGGAGC
AATTTGAAGCCAATTTCCATCGCCTGGCGATCGCTCCATTCTTGGCTATATGTTTTTCACCGTTCCCGGGGC
CATTTTCAAAGACTCGTCGGTAAGATAAGATTGTGTCACTCGCTGTCTCTCTTCATTTGTCGAAGAATGCTG
AGGAATTTCGCGATGACGTCGGCGAGTATTTTGAAGAATGAGAATAATTTGTATTTATACGAAAATCAGTTA
GTGGAATTTTCTACAAAAACATGTTATCTATAGATAATTTTGTTGCAAAATATGTTGACTATGACAAAGATT
GTATGTATATACCTTTAATGTATTCTCATTTTCTTATGTATTTATAATGGCAATGATGATACTGATGATATT
TTAAGATGATGCCAGACCACAGGCTGATTTCTGCGTCTTTTGCCGAACGCAGTGCATGTGCGGTTGTTGTTT
TTTGGAATAGTTTCAATTTTCGGACTGTCCGCTTTGATTTCAGTTTCTTGGCTTATTCAAAAAGCAAAGTAA
AGCCAAAAAAGCGAGATGGCAATACCAAATGCGGCAAAACGGTAGTGGAAGGAAAGGGGTGCGGGGCAGCGG
AAGGAAGGGTGGGGCGGGGCGTGGCGGGGTCTGTGGCTGGGCGCGACGTCACCGACGTTGGAGCCACTCCTT
TGACCATGTGTGCGTGTGTGTATTATTCGTGTCTCGCCACTCGCCGGTTGTTTTTTTCTTTTTATCTCGCTC
TCTCTAGCGCCATCTCGTACGCATGCTCAACGCACCGCATGTTGCCGTGTCCTTTATGCGTCATTTTGGCTC
GAAATAGGCAATTATTTAAACAAAGATTAGTCAACGAAAACGCTAAAATAAATAAGTCTACAATATGGTTAC
TTATTGCCATGTGTGTGCAGCCAACGATAGCAACAAAAGCAACAACACAGTGGCTTTCCCTCTTTCACTTTT
TGTTTGCAAGCGCGTGCGAGCAAGACGGCACGACCGGCAAACGCAATTACGCTGACAAAGAGCAGACGAAGT
TTTGGCCGAAAAACATCAAGGCGCCTGATACGAATGCATTTGCAATAACAATTGCGATATTTAATATTGTTT
ATGAAGCTGTTTGACTTCAAAACACACAAAAAAAAAAATAAAACAAATTATTTGAAAGAGAATTAGGAATCG
GACAGCTTATCGTTACGGGCTAACAGCACACCGAGACGAAATAGCTTACCTGACGTCACAGCCTCTGGAAGA
ACTGCCGCCAAGCAGAGAGAGAGAGAAAAAGAGGGAGAGCAGCTTAGACCGCATGTGCTTGTGTGTGAGGCG
TCTCTCTCTTCGTCTCCTGTTTGCGCAAACGCATAGACTGCACTGAGAAAATCGATTACCTATTTTTTATGA
ATGAATATTTGCACTATTACTATTCAAAACTATTAAGATAGCAATCACATTCAATAGCCAAATACTATACCA
CCTGAGCGATGCAACGAAATGATCAATTTGAGCAAAAATGCTGCATATTTAGGACGGCATCATTATAGAAAT
GCTTCTTGCTGTGTACTTTTCTCTCGTCTGGCAGCTGTTTCGCCGTTATTGTTAAAACCGGCTTAAGTTAGG
TGTGTTTTCTACGACTAGTGATGCCCCTACTAGAAGATGTGTGTTGCACAAATGTCCCTGAATAACCAATTT
GAAGTGCAGATAGCAGTAAACGTAAGCTAATATGAATATTATTTAACTGTAATGTTTTAATATCGCTGGACA
TTACTAATAAACCCACTATAAACACATGTACATATGTATGTTTTGGCATACAATGAGTAGTTGGGGAAAAAA
TGTGTAAAAGCACCGTGACCATCACAGCATAAAGATAACCAGCTGAAGTATCGAATATGAGTAACCCCCAAA
TTGAATCACATGCCGCAACTGATAGGACCCATGGAAGTACACTCTTCATGGCGATATACAAGACACACACAA
GCACGAACACCCAGTTGCGGAGGAAATTCTCCGTAAATGAAAACCCAATCGGCGAACAATTCATACCCATAT
ATGGTAAAAGTTTTGAACGCGACTTGAGAGCGGAGAGCATTGCGGCTGATAAGGTTTTAGCGCTAAGCGGGC
TTTATAAAACGGGCTGCGGGACCAGTTTTCATATCACTACCGTTTGAGTTCTTGTGCTGTGTGGATACTCCT
CCCGACACAAAGCCGCTCCATCAGCCAGCAGTCGTCTAATCCAGAGACCCCGGATCCGATGGGGTACCTACT
AGTCCAGTGTGGTGGAATTCTGCAGATATCCAGCACAGTGGCGGCCGCTCGAGTCTAGAGGGCCCTTCGAAG
GTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCATCACCATCACCATTGAG
TTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAAGGCCTGAGCTCGCTGATCAGCCTCGATCGAGGATC
CAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTT
GTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATT
GCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAAT
GTGGTATGGCTGATTATGATCAGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGG
GCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCG
GCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCG
GTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAG
CAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGG
ATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCC
AGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCAGCAAAAGGCC
AGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAAT
CGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCC
CTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTG
GCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTG
CACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGA
CACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACA
GAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAG
CCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT
TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGG
TCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC
TAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGT
TACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTC
CCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAC
CCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCT
GCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAAT
AGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTC
AGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTC
GGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAAT
TCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAA
TAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACT
TTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCC
AGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGA
GCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTC
TTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATT
TAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATT
ATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGT

pCH2	pET-32a(+)
pdKeima-Red-S1	pCRE-DD-AmCyan1
pKLAC1	pPIC ZαGB
pET-41a(+)	pIC20H
pEF1/V5-HisA	pHY15
pSC101	pML21
pNEO	pALEX a,b,c
pHS85	pUG6
pCREj-2	pcDNA5/FRT
pENTR11-Dual	pBD-NF-kB
EWD299	pUC4-KISS
pcDNA4/HisMax B	pMDLg/pRRE
pCas9D10A-GFP	pSET152
pMT-Bip-V5-HisA	pBBR1MCS-2
pFLAG-CMV2	pNEO-ars
pACT-MyoD	pTP-4
pDC515	pDEST26
pcDNA3.1(+)/CAT	pIJ702-del35-55
pHV23	pWF1
pLVX-DD-ZsGreen1 Control	pATPase_TA
pMal-c4X	pHSG422
pAcGFP1-N2	pOrf681_TA
pAD-SV40T	pSH65
pPIC9k-His	pMal-c2G
c-Flag pcDNA3	pCL-Ampho
pHB-1	pGL4.72[hRlucCP]
pcDNA6.2-DsRed2-MCS1 miR	PX543
pSUPER.neo+gfp	pAGO
pBT-4	pFLAG-CMV-3
pTi	pCDH-CMV-MCS-EF1a-RFP+BSD
pCGN978	pCYM1
pSP72	pSUMO
pET-28a-BFP-C	pLexm
pET-12c(+)	pVPack-GP
pGL4.38[luc2P/p53 RE/Hygro]	pPIC9
pAN7-1	pFA1
ISceI-GR-RFP	pProLabel-C
pREP4	pBluescript II KS(-)
pcDNA3.1-GP130v1	pRSET-EGFP
pGL4.45[luc2P/ISRE/Hygro]	pGL4.41[luc2P/HSE/Hygro]
pUCTag	pLNCX2
pET-21a(+)	pOM2
pCRE-MetLuc2-Reporter	pRB373
pBad43	pDsRed-Monomer
pLLP-OmpA	pSC101-EcoRImeth+
pL-brevisGH3-MBP	Pgex-HRV3C
pJSC73	pMTD1.3xA
pET-32 EK/LIC	pEF6/myc-His B
pcDNA5/FRT/V5-His-TOPO	pBAD18
pTet-Splice	pIκB-EGFP

pUCC001	pMXs-hOCT4 (Plath)
pCMV-MMLVgag-3xNES-ABE8e	pCW-Cas9
pAc-Neo-OVA	LeGO-G
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV	pTEF:ATP
pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-I	MSCV-(pBabe mcs)- human p210BCR-ABL-IRES
TAL3393	pCXLE-hOCT3/4
pGGA008	pDIRECT_23D
800 pSG5L HA PTEN wt	pMSCV-hygro-STING
pLVX-M-puro	pAraGFPCDF
pCDH-puro-Bcl-XL	pGH335_MS2-AID*Δ-Hygro
pSR47	pEGFP-LC3
c-myc-PT3EF1a	pM-Cas9
pFC334	pAAVS1-PDi-CRISPRn
pSBtet-GP	pBHt2
EC2_3_dCas9_Mxi1_sgRNA	pAAVS1-PC-GCaMP6f
pNN9	pT4-CMV-GFP
pMSCV-IRES-GFP II (pMIG II)	Cbh_v5 AAV-CBE N-terminal
PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a	p201G Cas9
pT3-Neo-EF1a-GFP	pGL3 2 kb prom. CD274
pICH86966::AtU6p::sgRNA_PDS	Lenti-luciferase-P2A-Neo
pLentiV2-dCas9-VP64	Tet-O-FUW-Neurod1
pRC-CMV-Rev1b	GST-HA-GFP11-N1
pLenti-EML4-ALK variant 3a	pC003 - LshC2C2 locus into pACYC184 for
pRAD1	401 pSG5L
pJKR-L-tetR	pMonAID_nCas9-PmCDA_Hyg_ALS
pAAV2/8	pXAT2
EC1000	PB_tre_Cas9
pLenti-DsRed_IRES_MAPT:EGFP	phage UbiC NLS HA stdMCP stdHalo
ilux pGEX(-)	pCAG-GBP1-10gly-lexADBD
pcDNA3.4_7C11-1 heavy chain	pMJ329: pCMV-MmeFz2
NM9-SpCas9-NLS3	pHAGE-PTCH1
pSELECT-HA-mFOXO1	pCS2TAL3-RR
1306 pSG5L HA FKHR wt	pTRKH2
AbVec1.1-IGKC	pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK
pEGFP-puro	pAAV-EFS-CjCas9-eGFP-HIF1a
VVT1	pJSC131 - Bacterial expression plasmid f
pAc-sgRNA-Cas9	pCS2TAL3-DD
pET28a-At-TRL	FUmGW
pCAS9-mCherry empty	p58sheLL
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-	pAAV-EF1a-FLEX-GTB
pYES-mtGFP	pLenti X2 Puro DEST (w16-1)
3XMEF2-luc	BPK3082
LB1366 (Bcl-2 promoter with MHS1234)	pPPEM
BECLIN-HA WT	pNEU-hMbPylRS-4xU6M15
EC2_10_dCas9_VPR_HC_sgRNA	pCMV-hA3G-BE3
pM1s3AsR_TS-C7	pMal-T-Avi-His/BirA
CKS2 gRNA (BRDN0001149143)	pNK9
pLJC5-3XHA-EGFP-PEX26	pAC1404-pCR8-mRuby2_NLSPUFa
pFRT/TO/GFP-SCAF4 (CRISPR_resistant)	pQUAST>stop>mCD8-GFP
pNeae2	pTRE Tight ArchT-GFP

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pAc5/V5-HisA

买家导航

pAc5/V5-HisA载体质粒基本信息

pAc5/V5-HisA质粒图谱载体图谱和pAc5/V5-HisA载体序列质粒序列多克隆位点信息

pAc5/V5-HisA质粒载体简介

pAc5/V5-HisA质粒序列载体序列

pAc5/V5-HisA载体图谱质粒图谱pdf版和pAc5/V5-HisA载体序列质粒序列等相关资料下载

pAc5/V5-HisA质粒载体应用举例

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