| 质粒名称: | pCMV_AncBE4max |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 112094 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 8961 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，CRISPR |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | AncBE4max |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 5562 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | T7 Forward |
| 3’测序引物 | BGH-Reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV_AncBE4max was a gift from David Liu (Addgene plasmid # 112094 ; http://n2t.net/addgene:112094 ; RRID:Addgene_112094) |
| 参考文献部分: | Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP, Raguram A, Liu DR. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. 10.1038/nbt.4172 PubMed 29813047 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV_AncBE4max 8961 bp ds-DNA circular SYN 14-FEB-2022
DEFINITION C:G-to-T:A base editing.
ACCESSION .
VERSION .
KEYWORDS pCMV_AncBE4max
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8961)
AUTHORS Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP,
Raguram A, Liu DR
TITLE Improving cytidine and adenine base editors by expression
optimization and ancestral reconstruction.
JOURNAL Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
PUBMED 29813047
REFERENCE 2 (bases 1 to 8961)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 14, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8961
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 3..251
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 282..530
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 573..593
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 602..619
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(642..659)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 648..872
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(943..959)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(943..959)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(956..978)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 967..983
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(991..1021)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1036..1057
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1174..1191)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1345..1933)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1425..1444)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2104..2964)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2727..2746
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2965..3069)
/gene="bla"
/label=AmpR promoter
primer_bind complement(3148..3167)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 3339..3718
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3719..3922
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3873..3893
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 3964..3983
/label=T7
/note="T7 promoter, forward primer"
promoter 3964..3982
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 4032..4052
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4833..8933
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
1 gcactaatct gagcgacatc attgagaagg agactgggaa acagctggtc attcaggagt
61 ccatcctgat gctgcctgag gaggtggagg aagtgatcgg caacaagcca gagtctgaca
121 tcctggtgca caccgcctac gacgagtcca cagatgagaa tgtgatgctg ctgacctctg
181 acgcccccga gtataagcct tgggccctgg tcatccagga ttctaacggc gagaataaga
241 tcaagatgct gagcggagga tccggaggat ctggaggcag caccaacctg tctgacatca
301 tcgagaagga gacaggcaag cagctggtca tccaggagag catcctgatg ctgcccgaag
361 aagtcgaaga agtgatcgga aacaagcctg agagcgatat cctggtccat accgcctacg
421 acgagagtac cgacgaaaat gtgatgctgc tgacatccga cgccccagag tataagccct
481 gggctctggt catccaggat tccaacggag agaacaaaat caaaatgctg tctggcggct
541 caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagaggaaa gtctaaccgg
601 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag
661 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
721 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
781 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
841 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
901 ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
961 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
1021 agcctaggat gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
1081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcgggaag
1141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
1201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
1261 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
1321 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
1381 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
1441 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
1501 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
1561 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
1621 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
1681 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
1741 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
1801 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
1861 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
1921 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacactc agtggaacga
1981 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
2041 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
2101 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
2161 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
2221 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
2281 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
2341 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
2401 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
2461 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
2521 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
2581 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
2641 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
2701 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
2761 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
2821 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
2881 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
2941 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
3001 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
3061 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc
3121 tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca
3181 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
3241 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
3301 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
3361 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
3421 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
3481 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
3541 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
3601 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
3661 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
3721 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
3781 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
3841 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
3901 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctaga gatccgcggc
3961 cgctaatacg actcactata gggagagccg ccaccatgaa acggacagcc gacggaagcg
4021 agttcgagtc accaaagaag aagcggaaag tcagcagtga aaccggacca gtggcagtgg
4081 acccaaccct gaggagacgg attgagcccc atgaatttga agtgttcttt gacccaaggg
4141 agctgaggaa ggagacatgc ctgctgtacg agatcaagtg gggcacaagc cacaagatct
4201 ggcgccacag ctccaagaac accacaaagc acgtggaagt gaatttcatc gagaagttta
4261 cctccgagcg gcacttctgc ccctctacca gctgttccat cacatggttt ctgtcttgga
4321 gcccttgcgg cgagtgttcc aaggccatca ccgagttcct gtctcagcac cctaacgtga
4381 ccctggtcat ctacgtggcc cggctgtatc accacatgga ccagcagaac aggcagggcc
4441 tgcgcgatct ggtgaattct ggcgtgacca tccagatcat gacagcccca gagtacgact
4501 attgctggcg gaacttcgtg aattatccac ctggcaagga ggcacactgg ccaagatacc
4561 cacccctgtg gatgaagctg tatgcactgg agctgcacgc aggaatcctg ggcctgcctc
4621 catgtctgaa tatcctgcgg agaaagcagc cccagctgac atttttcacc attgctctgc
4681 agtcttgtca ctatcagcgg ctgcctcctc atattctgtg ggctacaggc ctgaagtctg
4741 gaggatctag cggaggatcc tctggcagcg agacaccagg aacaagcgag tcagcaacac
4801 cagagagcag tggcggcagc agcggcggca gcgacaagaa gtacagcatc ggcctggcca
4861 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
4921 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
4981 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
5041 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
5101 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
5161 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
5221 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc
5281 tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg
5341 agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
5401 cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca
5461 tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg
5521 gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca
5581 acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct
5641 acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc
5701 tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg
5761 agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg
5821 acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt
5881 tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag
5941 agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg
6001 tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc
6061 cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc
6121 cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact
6181 acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg
6241 aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct
6301 tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc
6361 acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga
6421 ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc
6481 tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga
6541 aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc
6601 tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg
6661 aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga
6721 tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc
6781 tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc
6841 gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca
6901 gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
6961 cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc
7021 ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga
7081 tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc
7141 agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
7201 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
7261 tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca
7321 accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact
7381 ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc
7441 cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc
7501 tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac
7561 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg
7621 tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc
7681 gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc
7741 agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctaaacg
7801 ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg
7861 gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca
7921 aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta
7981 ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg
8041 agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc
8101 aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc
8161 tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt
8221 acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa
8281 agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa
8341 gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga
8401 aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga
8461 agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca
8521 aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg
8581 ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg
8641 agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc
8701 tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc
8761 acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca
8821 tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga
8881 gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggt gacagcggcg
8941 ggagcggcgg gagcgggggg a
//
