| 培养基名称 | 改良Giolitti-Cantoni肉汤，Giolitti-Cantoni肉汤（ISO标准 ） |
|---|---|
| 英文名称: |
Modified Giolitti and Cantoni Broth (ISO) |
| 培养基类型: | 选择性培养基 |
| 产品目录号: | M731-01 |
| 产品规格: |
250g 其它规格请联系我们 |
| 保存条件: | 密封，阴凉干燥处保存。制备好的培养基2 - 8°C避光保存。 |
| 产品性状: |
麦秸色均一粉末。 麦秸色透明溶液。 |
| 注意事项: | 避免摄入、呼入、皮肤接触。配制时在通风橱中进行，戴口罩 、 手套、护目镜。 |
| 相关产品: | -- |
产品描述：
Giolitti-Cantoni肉汤基础(Modified Giolitti-Cantoni Broth) 是选择性液体培养基，是ISO 6888-3:2003规定的MPN法检测和增菌食品和饲料中的凝固酶阳性葡萄球菌 用培养基。本配方是ISO标准配方。
Giolitti-Cantoni肉汤工作原理
牛肉提取物、酵母提取物和胰蛋白胨提供氮源和生长因子。丙酸钠、 甘氨酸和高浓度甘露醇刺激目的菌的生长和修复，氯化锂是选择性抑制剂，抑制革兰氏阴性杆菌，亚碲 酸钾与甘氨酸抑制除葡萄球菌外其它革兰氏阳性菌，尤其是芽孢杆菌生长。厌氧培养可增强选择性，在 培养基表面加2cm深石蜡油能够抑制微球菌的生长。亚碲酸盐同时也是鉴别指示剂，金黄色葡萄球菌能够 还原亚碲酸盐，生成黑色的碲。如果培养基颜色变黑，则认为是金葡菌阳性。将菌液接种到金葡菌分离 培养基如Baird-Parker琼脂上。
Giolitti-Cantoni肉汤的用途：
改良Giolitti-Cantoni肉汤培养基（Modified Giolitti and Cantoni Broth ）被ISO 6888-3:2003规定的MPN法检测和增菌食品和饲料中的凝固酶阳性葡萄球菌用培 养基。
本培养基是回收受伤葡萄球菌或从低含量样本中回收葡萄球菌的最佳培养基。
Giolitti-Cantoni肉汤培养基配方与配制方法
| 培养基成分： | 含量：g/L |
|---|---|
| 胰蛋白胨 | 10.0 |
| 酵母提取物 | 5.0 |
| 牛肉提取物 | 5.0 |
| 氯化锂 | 5.0 |
| 甘露醇 | 20.0 |
| 氯化钠 | 5.0 |
| 甘氨酸 | 1.2 |
| 丙酸钠 | 3.0 |
| pH | 6.9± 0.2 |
配制方法：
1. 称取54.2g本品，加入1L去离子水，1ml吐温-80，温和加热溶解。
2. 分装19ml至20 x 200mm试管中。或者分装9ml至16 x 160mm试管中。
3. 121°C灭菌15min。
4. 快速冷却，加入0.1ml无菌1%亚碲酸钾溶液到9ml培养基中（工作浓度0.1g/L）。
实验方法
The medium should be inoculated as soon as it has been cooled after autoclaving. If there is a delay in putting the medium to use it must be freed from dissolved air by immersion in free-flowing steam for 20 minutes.
Inoculate 1g of sample material and 1ml aliquots of a series of suitable decimal dilutions into tubes containing 19 ml of Giolitti-Cantoni Broth. Two tubes are used for the sample material and for each of the dilutions. This increases the likelihood of detecting Staphylococcus aureus when it is present in very small numbers.
The medium is overlaid with 2cm of molten sterile paraffin wax (melting temperature 42 -44°C) and incubated for 48 hours at 35°C, examining daily. The result is considered negative for Staphylococcus aureus if no blackening of the medium is observed. If blackening does occur at the bottom of the tubes or general blackening of the medium, the broth is streaked on to a staphylococcal isolation medium, such as Baird-Parker Medium8 and incubated at 35°C for 24-48 hours. The result is considered positive if black colonies, with a narrow white margin, surrounded by a zone of clearing, are seen.
结果与分析
标准菌株 生长情况
金葡菌 ATCC25923 生长极好，培养基变黑
