| 产品名称： | Mossel肠杆菌增菌肉汤；肠杆菌增菌肉汤（Mossel） |
|---|---|
| 英文名称: | Enterobacteria Enrichment Broth-Mossel |
| 培养基类型: | 选择性培养基 |
| 级别: | for microbiology |
| 品牌: | ELITE-MEDIA |
| 产品目录号: | M1062-01、M1062-02、M1062-03 |
| 产品规格: | 250g、500g、2.5kg |
| 产品外观: | 浅绿色，自由流动粉末。 |
| 颜色与澄清度: | 绿色溶液。 |
| 保存条件: | 密封，2-25度保存；预制培养基2-8°C或室温条件下保存 |
| 注意事项: | 避免摄入、呼入、皮肤接触 |
| 相关产品: | -- |
产品描述：
Mossel肠杆菌增菌肉汤(Enterobacteria Enrichment Broth-Mossel)是耐胆盐的革兰氏阴性细菌的增菌培养基，应用于药品的微生物学检测。本培养基配方克服了由于胆盐的多种形式造成的对一小部分胆盐耐受型革兰氏阴性菌抑制效果不理想的问题。纯化胆盐的使用排除了这些问题，初步分析结果可以用于通过每个单位体积培养基大约接种1个活细胞的方式检测生长速度。Mossel肠杆菌增菌肉汤培养基应该作为增菌肉汤与紫红胆盐葡萄糖琼脂（Violet Red Bile Glucose Agar）结合使用。
用途：
Mossel肠杆菌增菌肉汤可以用于缺失测试或者定量试验中。Mossel肠杆菌增菌肉汤用于耐胆盐的革兰氏阴性细菌的增菌。
配方与配制方法：
| 成分 | g/l |
| 明胶蛋白胨 | 10.0 |
| 一水葡萄糖 | 5.0 |
| 纯化胆盐 | 3.055(20.0) |
| 磷酸二氢钾，无水 | 2.0 |
| 磷酸氢二钠，无水 | 6.4 |
| 亮绿 | 0.015 |
| pH 7.2 ± 0.2 |
用1L纯水溶解26.47g 本产品。
分装到250ml锥形瓶中，每瓶100ml培养基。
用100°C流动蒸汽加热30min。
在流动的冷水中快速降温。
不要高温蒸汽灭菌！
实验方法：
1. Sample preparation and pre-incubation Prepare a sample using a 1 in 10 dilution of not less than 1g of the product to be examined as described in general chapter EP 2.6.12 (Harmonised method), but using casein soya bean digest broth (CM0129) as the chosen diluent, mix and incubate 20-25°C for a time sufficient to resuscitate the bacteria but not sufficient to encourage multiplication of the organisms (usually 2 hours but not more than 5 hours).
2. Test for absence
Unless otherwise prescribed, use the volume corresponding to 1g of the product, as prepared in 1, to inoculate Enterobacteria Enrichment Broth-Mossel (CM1115). Incubate at 30-35°C for 24-48 hours. Subculture on plates of Violet Red Bile Glucose Agar (R455302). Incubate at 30-35°C for 18-24hours. The product complies with the test if there is no growth of colonies.
3. Quantitative test
(1). Selection and subculture: inoculate suitable quantities of Enterobacteria Enrichment Broth Mossel (CM1115) with the preparation as described under 1 and/or dilution of it containing respectively 0.1g, 0.01g and 0.001g (or 0.1ml, 0.01ml and 0.001ml) of the product to be examined. Incubate at 30-35°C for 24-48 hours. Subculture each of the cultures on a plate of Violet Red Bile Glucose Agar (R455302). Incubate at 30-35°C for 18-24 hours.
(2). Interpretation: growth of colonies constitutes a positive result. Note the smallest quantity of the product that gives a positive result and the largest quantity that gives a negative result. Determine from the table below the probable number of bacteria.
