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抗真菌剂测定琼脂；杀真菌剂测定培养基 |
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Antifungal Assay Agar |
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非选择性培养基 |
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for microbiology |
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ELITE-MEDIA（艾礼培养基） |
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M297-01、M297-02 |
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250g、500g |
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麦秸色粉末。 |
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浅黄色略不透明凝胶。 |
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密封，2-25°C保存。 |
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避免摄入、吸入、皮肤接触。 |
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产品描述：
抗真菌测定琼脂（Antifungal Assay Agar）用于使用管碟法（牛津杯法）或纸片扩散法测定药品和其它 材料的抗真菌活性。
工作原理
真菌感染在过去的十年中急剧增加，经常伴随系统性感染，如AIDS、癌症患者和免疫能力降低的病人。不 幸的是，抗真菌药物种类很少，真菌感染趋于快速产生对这些药物的耐药性。真菌感染的致死率远高于细菌感染。因此，抗真菌剂的增加和抗 真菌剂的需求的增加，急需更快、更准确的抗真菌药物筛选方法和药敏试验（susceptibility testing）方法。抗真菌剂测定琼脂由Berger 和Lazecka研发，既作为基础琼脂也作为种子琼脂，用于便捷地测定药品和其它材料的抗真菌活性。该培养基是合成培养基，成分是已知的， 因此抑制性只能来自抗真菌剂，而不是养分耗竭。
用途：
抗真菌测定琼脂被推荐用于测定药品和其它材料的抗真菌活性。
配方与配制方法
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成分 |
(g/L) |
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葡萄糖 |
50.000 |
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柠檬酸钠 |
4.500 |
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磷酸氢二钠 |
0.550 |
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柠檬酸 |
1.000 |
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酪蛋白胨 |
4.000 |
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盐酸吡哆辛 |
0.00025 |
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维生素B1 |
0.00025 |
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肌醇 |
0.025 |
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泛酸钙 |
0.0025 |
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烟酸 |
0.0025 |
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氯化钾 |
0.425 |
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氯化钙 |
0.125 |
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硫酸镁 |
0.125 |
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氯化铁 |
0.0025 |
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硫酸镁 |
0.0025 |
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生物素 |
0.000008 |
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琼脂 |
15.000 |
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pH 5.5±0.2 |
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配制方法：
1. 称取75.76g本产品，用1000ml纯水重悬。
2. 加热使培养基完全溶解。
3. 121°C 灭菌15min。
4. 冷却至45-50°C 时倒平板。
实验方法
Cylinder plate method: This method was first devised by Abraham et al (4) and later modified by Schmidt and Moyer (5) and it depends upon diffusion of the antibiotic from vertical steel cylinders placed on the surface of inoculated agar medium. This produces zones of inhibition around the cylinder containing antibiotic solution depending upon the concentration of the antibiotic in the cylinder. This method is commonly employed in the assay of pharmaceutical preparations of Penicillin and other antibiotics. For assay, use Petri plates with 20 x100 mm dimension and stainless steel or porcelain cylinders with the outside diameter 8 mm, inside diameter 6 mm and length 10 mm. All dimensions should have a tolerance of 0.1 mm. The cylinders should be carefully cleaned to remove all the impurities. For assays requiring base and seed layer, the base layer is allowed to solidify first and then overlaid with the seed agar containing the proper concentration of the test organism. Most assays require base layer of 21 ml and seed layer of 4 ml. Generally 6 cylinders are used per plate. The cylinders are placed on inoculated plates at equal distance.
Paper-disc method: Paper discs with a diameter of 9 mm are impregnated with the antibiotic solution and placed on the culture medium. Antibiotic can also be applied to the disc after it has been placed on the medium. Plates containing a single layer of medium with 2 mm thickness may be used for these tests. All other steps are similar to the cylinder plate method.
