| 产品名称： | Gibco DMEM培养基、Gibco DMEM高糖不含谷氨酰胺无酚红培养基 |
|---|---|
| 产品又名： | Gibco DMEM细胞培养基、Gibco DMEM高糖不含谷氨酰胺无酚红细胞培养基 |
| 英文名称： | Gibco DMEM, high glucose, no glutamine, no phenol red |
| 培养基类型： | 哺乳细胞培养基 |
| 级别： | For Mammalian Cell Culture |
| 品牌： | Gibco |
| 产品目录号： | 31053028、31053-028 |
| 葡萄糖: | 高糖 |
| 葡萄糖浓度: | 4500 mg/L |
| 谷氨酰胺： | 不含L-谷氨酰胺 |
| 酚红： | 不含酚红 |
| 钠： | 不含钠 |
| HEPES | 不含HEPES |
| 环保特征: | 可循环使用包装 |
| 产品规格： | 500ml |
| 血清添加: | 使用时，正常浓度添加血清。 |
| 运输条件： | 室温 |
| 产品外观： | 透明液体 |
| 保存条件： | 密封，2-8°C保存。 |
| 注意事项： | 避免呼入和皮肤接触。 |
| Gibco： |
新鲜酵母浸出液 热灭活马血清 |
Gibco DMEM培养基图片
Gibco DMEM细胞培养基产品描述：
DMEM(Dulbecco's Modified Eagle Medium)是一种广泛应用的基础培养基，用于支持多种哺乳动物细胞的生长。在DMEM中成功培养的细胞包括原代成纤维细胞、神经元、胶质细胞、HUVECs和平滑肌细胞，以及HeLa、293、Cos-7和PC-12等细胞系。Life Technologies为一系列细胞培养应用提供了多种DMEM培养基。Gibco DMEM培养基使用方法
与其他培养基相比，DMEM是独特的，因为它含有4倍的氨基酸和维生素的浓度比原来的EMEM培养基。DMEM最初是用低葡萄糖（1 g/L）和钠配制的，但通常与高葡萄糖水平一起使用，有或没有钠。DMEM不含蛋白质、脂类或生长因子。因此，DMEM需要补充，通常添加10%的胎牛血清（FBS）。DMEM使用碳酸氢钠缓冲系统（3.7 g/L），因此需要5–10%的CO2环境来维持生理pH值。Gibco DMEM培养基质量生产与质量体系
DMEM由位于纽约格兰德岛的cGMP兼容标准工厂生产。该设施在FDA注册为医疗器械制造商，并通过ISO 13485标准认证。为保持供应链的连续性，Life Technologies提供了在苏格兰工厂（41965-039）生产的相同DMEM产品。该工厂在FDA注册为医疗器械制造商，并通过ISO 13485标准认证。Gibco DMEM培养基配方
| Components | Molecular Weight | Concentration (mg/L) | mM |
| Amino Acids | |||
| Glycine | 75 | 30 | 0.4 |
| L-Arginine hydrochloride | 211 | 84 | 0.39810428 |
| L-Cystine 2HCl | 313 | 63 | 0.20127796 |
| L-Histidine hydrochloride-H2O | 210 | 42 | 0.2 |
| L-Isoleucine | 131 | 105 | 0.8015267 |
| L-Leucine | 131 | 105 | 0.8015267 |
| L-Lysine hydrochloride | 183 | 146 | 0.7978142 |
| L-Methionine | 149 | 30 | 0.20134228 |
| L-Phenylalanine | 165 | 66 | 0.4 |
| L-Serine | 105 | 42 | 0.4 |
| L-Threonine | 119 | 95 | 0.79831934 |
| L-Tryptophan | 204 | 16 | 0.078431375 |
| L-Tyrosine disodium salt dihydrate | 261 | 104 | 0.39846742 |
| L-Valine | 117 | 94 | 0.8034188 |
| Vitamins | |||
| Choline chloride | 140 | 4 | 0.028571429 |
| D-Calcium pantothenate | 477 | 4 | 0.008385744 |
| Folic Acid | 441 | 4 | 0.009070295 |
| Niacinamide | 122 | 4 | 0.032786883 |
| Pyridoxine hydrochloride | 206 | 4 | 0.019417476 |
| Riboflavin | 376 | 0.4 | 0.00106383 |
| Thiamine hydrochloride | 337 | 4 | 0.011869436 |
| i-Inositol | 180 | 7.2 | 0.04 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111 | 200 | 1.8018018 |
| Ferric Nitrate (Fe(NO3)3"9H2O) | 404 | 0.1 | 2.48E-04 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120 | 97.67 | 0.8139166 |
| Potassium Chloride (KCl) | 75 | 400 | 5.3333335 |
| Sodium Bicarbonate (NaHCO3) | 84 | 3700 | 44.04762 |
| Sodium Chloride (NaCl) | 58 | 6400 | 110.344826 |
| Sodium Phosphate monobasic (NaH2PO4-H2O) | 138 | 125 | 0.9057971 |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 4500 | 25 |
Gibco细胞培养基选择指南
| Transfection Reagent （细胞转染试剂） | ||
|---|---|---|
| 产品描述 | 规格 | 货号 |
|
Lipofectamin 2000 （Lipo2000、Lipo 2000） Each unit contains one vial of Lipofectamine 2000 Reagent. |
0.75 ml |
11668-027 11668027 |
|
Lipofectamin 3000 （Lipo3000、lipo 3000） Contains one vial of Lipofectamine 3000 Reagent and one vial of P3000TM Enhancer Reagent. |
0.75 ml |
L3000-008 L3000008 |
| Dulbecco’s Modified Eagle Media （DMEM培养基） | ||
| 产品描述 | 规格 | 货号 |
|
DMEM (1X), liquid (high glucose) Contains no L-glutamine or sodium pyruvate |
500 mL |
11960-044 11960044 |
|
DMEM (1X), liquid (high glucose)
Contains L-glutamine, but no sodium pyruvate
|
500 mL |
11965-092 11965092 |
|
DMEM (1X), liquid (high glucose) (DMEM高糖培养基，最常用)
Contains L-glutamine and sodium pyruvate
|
500 mL |
11995-065 11995065 |
|
DMEM (1X), liquid (high glucose)
Contains 110mg/L sodium pyruvate, but no L-glutamine
|
500 mL |
10313-021 10313021 |
|
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red
|
500 mL |
21063-029 21063029 |
|
DMEM (1X), liquid (high glucose)
Contains no L-glutamine, sodium pyruvate, or phenol red
|
500 mL |
31053-028 31053028 |
|
DMEM (1X), liquid (low glucose)
Contains L-glutamine and sodium pyruvate.
|
500 mL |
11885-084 11885084 |
|
DMEM (1X), liquid (high glucose)
Contains L-glutamine, and 25 mM HEPES, but no sodium pyruvate
|
500 mL |
12430-054 12430054 |
|
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, 15 mM HEPES butter, but no phenol red
|
500 mL |
11039-021 11039021 |
|
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, but no HEPES buffer
|
500 mL |
11320-033 11320033 |
|
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine and 15 mM HEPES buffer
|
500 mL |
11330-032 11330032 |
| RPMI Media （RPMI1640培养基） | ||
| 产品描述 | 规格 | 货号 |
|
RPMI 1640 (1X), liquid（最常用）
Contains L-glutamine
|
500 mL |
11875-093 11875093 |
|
RPMI 1640 (1X), liquid
Contains L-glutamine, but no phenol red
|
500 mL |
11835-030 11835030 |
|
RPMI 1640 (1X), liquid
Contains no L-glutamine
|
500 mL |
21870-076 21870076 |
|
RPMI 1640 (1X), liquid
Contains L-glutamine and 25 mM HEPES buffer
|
500 mL |
22400-089 22400089 |
| Minimum Essential Media （MEM培养基） | ||
| 产品描述 | 规格 | 货号 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts and L-glutamine
|
500 mL |
11095-080 11095080 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts and non-essential amino acids, but no L-glutamine
|
500 mL |
10370-021 10370021 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts, but no L-glutamine
|
500 mL |
11090-081 11090081 |
|
MEM α (1X), liquid
Contains L-glutamine, ribonucleosides, and deoxyribonuclosides
|
500 mL |
12571-063 12571063 |
|
MEM α Medium (1X), liquid
Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides
|
500 mL |
12561-056 12561056 |
|
MEM α Medium (1X), liquid
Contains L-glutamine, ribonucleosides and deoxyribonucleosides,
but no phenol red
|
500 mL |
41061-029 41061029 |
| GlutaMAX™ Media （GlutaMAX系列培养基） | ||
| 产品描述 | 规格 | 货号 |
|
DMEM (1X), liquid (high glucose)
Contains 110 mg/L sodium pyruvate
|
500 mL |
10569-010 10569010 |
|
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate
|
500 mL |
10564-011 10564011 |
|
DMEM (1X), liquid (high glucose)
Contains no sodium pyruvate
|
500 mL |
10566-016 10566016 |
|
DMEM (1X), liquid (low glucose)
Contains 110 mg/L sodium pyruvate
|
500 mL |
10567-014 10567014 |
|
DMEM/F12 (1X), liquid 1:1
Contains no HEPES buffer
|
500 mL |
10565-018 10565018 |
| F12 Nutrient Mixture (1X), liquid | 500 mL |
31765-035 31765035 |
|
IMDM (1X), liquid
Contains 25 mM HEPES buffer and 3,024 mg/L sodium bicarbonate
|
500 mL |
31980-030 31980-030 |
|
MEM α (1X), liquid （最常用）
Contains no ribonucleosides or deoxyribonucleosides
|
500 mL |
32561-037 32561037 |
|
MEM α (1X), liquid
Contains ribonucleosides and deoxyribonucleosides
|
500 mL |
32571-036 32571036 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts
|
500 mL |
41090-036 41090036 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts and 25 mM HEPES buffer
|
500 mL |
42360-032 42360032 |
| OptiMEM® I Reduced Serum Medium (1X), liquid | 500 mL |
51985-034 51985034 |
| RPMI 1640 (1X), liquid | 500 mL |
61870-036 61870036 |
|
RPMI 1640 (1x), liquid
Contains 25 mM HEPES buffer
|
500 mL |
72400-047 72400047 |
| 其他常用基础培养基 | ||
| 产品描述 | 规格 | 货号 |
|
F12 Nutrient Mixture (1X), liquid
Contains L-glutamine
|
500 mL |
11765-054 11765054 |
|
F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid
Contains L-glutamine
|
500 mL |
21127-022 21127022 |
|
IMDM (1X), liquid
Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol
|
12440-053 12440053 |
|
|
Liebovitz’s L-15 Medium (1X), liquid (L15 Medium)
Contains L-glutamine
|
11415-064 11415064 |
|
|
McCoy’s 5A Medium (modified) (1X), liquid
Contains L-glutamine
|
16600-082 16600082 |
|
|
Medium 199 (1X), liquid
Contains Earle’s salts, L-glutamine, and 2,200 mg/L sodium bicarbonate
|
11150-059 11150059 |
|
| Reduced Serum Media 细胞减血清培养基 | ||
| 产品描述 | 规格 | 货号 |
| Advanced D-MEM (1X), liquid | 500 mL |
12491-015 12491015 |
| Advanced D-MEM/F12 (1X), liquid | 500 mL |
12634-010 12634010 |
| Advanced MEM (1X), liquid | 500 mL |
12492-013 12492013 |
| Advanced RPMI 1640 (1X), liquid | 500 mL |
12633-012 12633012 |
| Opti-MEM®-I Reduced Serum Medium (1X), liquid | 500 mL |
31985-070 31985070 |
| Balanced Salt Solutions 细胞培养缓冲液 | ||
| 产品描述 | 规格 | 货号 |
|
DPBS (1X), liquid
Contains calcium and magnesium
|
500 mL |
14040-133 14040133 |
|
DPBS (1X), liquid
Contains no calcium or magnesium
|
500 mL |
14190-144 14190144 |
|
DPBS (10X), liquid
Contains no calcium or magnesium
|
500 mL |
14200-075 14200075 |
|
HBSS (1X), liquid
Contains calcium and magnesium, but no phenol red
|
500 mL |
14025-092 14025092 |
|
HBSS (1X), liquid
Contains no calcium or magnesium
|
500 mL |
14170-112 14170112 |
|
HBSS (1X), liquid
Contains no calcium chloride, magnesium chloride,
magnesium sulfate, or phenol red
|
500 mL |
14175-095 14175-095 |
| PBS 7.4 (1X), liquid | 500 mL |
10010-023 10010023 |
| PBS 7.4 (10X), liquid | 500 mL |
70011-044 70011044 |
| PBS 7.2 (1X), liquid | 500 mL |
20012-027 20012027 |
| PBS 7.2 (10X), liquid | 500 mL |
70013-032 70013032 |
| Insect Media 昆虫培养基 | ||
| 产品描述 | 规格 | 货号 |
|
Grace’s Insect Medium, Supplemented (1X), liquid
Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, and L-glutamine, but no insect hemolymph
|
500 mL |
11605-094 11605094 |
|
Schneider’s Drosophila Medium (1X), liquid
Contains L-glutamine
|
500 mL |
11720-034 11720034 |
|
SF900-II SFM (1X), liquid
Contains L-Glutamine, Insect cell culture tested
|
1000 mL |
10902-088 10902088 |
| Reagents 细胞培养试剂 | ||
| 产品描述 | 规格 | 货号 |
|
TrypLE™ Express Dissociation Enzyme with Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
|
100 mL |
12605-010 12605010 |
|
TrypLE™ Express Dissociation Enzyme without Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
|
100 mL |
12604-013 12604013 |
|
Trypsin-EDTA (0.25% Trypsin, EDTA•4Na) (1X), liquid（胰蛋白酶，最常用）
Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
|
100 mL |
25200-056 25200056 |
|
Trypsin-EDTA (0.05% Trypsin, EDTA•4Na) (1X), liquid
Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red |
100 mL |
25300-054 25300054 |
|
HEPES Buffer Solution (1M)
Contains 238.3 g/L prepared in distilled water
|
100 mL |
15630-080 15630080 |
| L-glutamine-200 mM (100X), liquid | 100 mL |
25030-081 25030081 |
| GlutaMAX™-I (100X), liquid | 100 mL |
35050-061 35050061 |
|
Antibiotic-Antimycotic (100X), liquid
10,000 units penicillin (base), 10,000 units streptomycin (base), and 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Fungizone™ Antimycotic in 0.85% saline
|
100 mL |
15240-062 15240062 |
|
Penicillin-Streptomycin, liquid （青链霉素双抗）
10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline
|
100 mL |
15140-122 15140122 |
| MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid | 100 mL |
11140-050 11140050 |
|
Distilled Water（无菌水）
Membrane filtered and endotoxin screened
|
500 mL |
15230-162 15230162 |
