pKL2299

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联系方式:I47-825O-882O

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pKL2299载体质粒基本信息

质粒名称: pKL2299
出品公司: Addgene
目录编号: 196291
存储实验室: Kan Wang

载体骨架基本信息
载体名称: pRK2-AraE
载体出品公司: Brian Pfleger
空载体大小: 3660bp
完整质粒大小: 29605bp
载体类型: 细菌表达
载体抗性: Gentamicin, 10 μg/mL
生长温度: 37 ℃
宿主菌: DH5a
拷贝数: 低拷贝

插入基因信息
插入基因名称: virulence genes from Agrobacterium tumefaciens pTiBo542
物种来源: Agrobacterium tumefaciens
插入大小: 25945bp
GenBank编号 DQ058764.1
克隆方法: Gibson Cloning
5’测序引物 cgttcggtcaaggttctgga
3’测序引物
aacacgcctgggtcaatgat
(Common Sequencing Primers)

文献引用方法
材料和方法部分: pKL2299 was a gift from Kan Wang (Addgene plasmid # 186332 ; http://n2t.net/addgene:186332 ; RRID:Addgene_186332)
参考文献部分:
An Improved Agrobacterium-Mediated Transformation and Genome-Editing Method for Maize Inbred B104 Using a Ternary Vector System and Immature Embryos. Kang M, Lee K, Finley T, Chappell H, Veena V, Wang K. Front Plant Sci. 2022 May 4;13:860971. doi: 10.3389/fpls.2022.860971. eCollection 2022. 10.3389/fpls.2022.860971 PubMed 35599865
 

序号

pKL2299质粒图谱载体图谱和pKL2299载体序列质粒序列多克隆位点信息

pKL2299载体图谱

pKL2299质粒载体简介

pKL2299质粒序列载体序列

LOCUS       pKL2299       29605 bp DNA     circular UNA 25-AUG-2024
DEFINITION  This plasmid carries the majority suite of Agrobacterium virulence 
            genes from Ti plasmid pTiBo542. The additional copies of vir genes 
            enhance Agrobacterium-mediated plant transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 29605)
  AUTHORS   Kang M, Lee K, Finley T, Chappell H, Veena V, Wang K
  TITLE     An Improved Agrobacterium-Mediated Transformation and Genome-Editing
            Method for Maize Inbred B104 Using a Ternary Vector System and 
            Immature Embryos.
  JOURNAL   Front Plant Sci. 2022 May 4;13:860971. doi: 
            10.3389/fpls.2022.860971. eCollection 2022.
  PUBMED    35599865
REFERENCE   2  (bases 1 to 29605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2024 from SnapGene Server 7.0.3
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2022-05-4"; volume: "13:860971. doi"; pages: " 
            10.3389/fpls.2022.860971. eCollection 2022"
FEATURES             Location/Qualifiers
     source          1..29605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3182..3190
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             16059..16070
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(26110..27258)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     IVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      27651..28182
                     /label=oriV
                     /note="incP origin of replication"
     terminator      complement(28294..28388)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(28659..29192)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEVVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(29381..29409)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttatccctaa tgcagagcca ccccatcagg aatatcgccc attccgtcat cagcgtggtg
       61 acgtgcatca ggcgcgcttg ttgcagaact ttaagtgctt ctttcgattc gagatctcag
      121 caaaagttct ccgcaaacct tcaccgcggg cttacgtgtc ttttgtaaca aattgcgacg
      181 cagttgatat ccacttgaaa cattagtccg aaattatcga gatctccgct gataaggtat
      241 cgaaatggcg ataaaattgg tattgatact cgtattcaca ctctttcccg cggcagacgc
      301 tgcatatgcg aatgaccgcg ccaacggttt catgtggtca aacgggggcg aaactggagt
      361 gaggcttcct cttcgggtgt tcaatgccaa gccagccaag aacacggtgg cgatcattta
      421 ttccggagac gctggatggc aaaatatcga tgaggcgatt ggtacctatc tgcagacgga
      481 agggattcct gtcattggcg tcagttcact tcggtatttc tggtcggagc ggtccccaag
      541 cgaaactgct aaggatcttg gtcacataat cgatgtctac accaagcatt tcggtgtgca
      601 gaatgtttta cttgtaggat attctttcgg cgcggacgtc atgccggcaa gcttcaatag
      661 gcttacgctt gagcaaaaaa atcgggttaa gcaaatctct ctcttggcat tgtcacatca
      721 agtcgactat gtcgtctcat ttaggggctg gctccaactc gaaacggaag gtaagggcgg
      781 caatcctctg gatgatctca gatccattga ccctgcaatc gtccaatgca tgtacgggcg
      841 cgaagaccgt aataatgctt gcccatctct ccgacagacc ggcgcagagg tgataggctt
      901 cagcggaggc catcactttg ataatgattt caaaaaactg tctacgcgcg tcgtctcagg
      961 cctcgtggca cgcctaagtc atcagtaatc tttagttcct gcaccgcttt agtattgact
     1021 gggatagcga cgcctgtgat gcagacatcg gatattgtgt cgttaagtaa aaggccttcg
     1081 tctgatcgcg agactcgcta gtggttttca ggtgagtgag atgttttgcc gcaagttgcg
     1141 ctgagatcgc atctgcctgc ggctgccgca cctccagatt ggcagcaaca agatcatcct
     1201 tcaagggaag atgcctataa cgcatgtgtt gacgactttc gcctcgtgaa tgatccggtc
     1261 tgttcccgac agtgggatgc cgtcgcgata gagcaggctc tcgcgccagg aggaatttct
     1321 ccgatatgac ttctctcctt ctacgtgccc aaagcagcaa cgaaatcatc tatcgcggcc
     1381 aaaagagcgg tcatgtgaaa gcacccttgc cagtcaattc cctggccaag gtcagcacgg
     1441 cgatactgcg aggtgttgct acaccacgga ggcgatctag aaggacgatt tcatcattta
     1501 ggccgcacac tcataaatca ggtttgcaaa tcggtctgat tttgattcat tgaggcttga
     1561 cttggaggcc actgataacc cgtccccgct ttgatgtcac cgatttcgaa tggactgttg
     1621 ttcagtcctt tgttcccaaa taagccgcgt cgcgtgccgc gcgttgacgc gggtgatcaa
     1681 tgtcatcttg tggcgcttcc ggacgggccc gccctgggca gacgttcctg atcgatacgg
     1741 ttcctgtaca acctgctaca atcgctttgt acgatggcgt gaggcagacg tctgggatca
     1801 tattctgagt gtgatttcca aggctttcga tagaatgtcg tcatgattga cagttcctgt
     1861 gtccgcgtcc accaacatgc ggcgacggga aacagcggga tcaagacgat ggctgtatgg
     1921 gacgttcccg tggcggtttg accaccaaga ttcacgctgt tgtcgatgcc gacggttgac
     1981 cgatccgtct tgcgctgaca gcgggtcaag cccatgaccg ccgcatgacc gaaacatttt
     2041 tacaaataat cgcttagagt gcgtttctgc tggcggacaa ggcatatgat accaacgcga
     2101 taagagcatt tgcaaagcgc aagcaggcat gggccaatat tcctgccaag agcaatcgga
     2161 aggaagcttc ccgttcatcg aatgggttta cagacagcgt aacctcgccg agcattttcg
     2221 ttctgagcct gacctgacca actgcggcct gaataggtcg atccggttgc ttagtcatgg
     2281 atgcgcggtt ctcggtccat gttgcgttcc aagacgccgg gcgaggtttc tcgcttcaat
     2341 tgaaatcata aagaagcaat tgaaaatttt cgagtaaccg accctcccga taatcttcaa
     2401 cataaaacaa cgcacttctt ccaacgggag aggcggtgtt agttgcgagc taaggagata
     2461 aggtatgctt aagagatcgg ggtcgctttc tcttgccttg atggtgagct tctgttcgtc
     2521 gagccttgcc acgccactct catctgctga gtttgaccat gttgctcgca agtgtgcccc
     2581 atcagttgcg acatctacgc ttgcggcgat agctaaggtg gagagtcgct ttgatccttt
     2641 agcgattcat gacaacacga ccggcgaaac gcttcactgg caagatcaca gccaagcaac
     2701 ccaagtcgtc aggcgccgtc tcgatgcacg gcattcgctg gatgttggcc tcatgcaaat
     2761 aaactctcga aatttttcta tgctcggtct gacacctgac ggtgcgctcc aggcgtgcac
     2821 atcattatct gccgctgcaa acatgctgaa aagtcgttat gcaggcggcg aaacgattga
     2881 cgagaagcaa tttgcgcttc gtcgggcgat ctccgcttac aacaccggta atttcatcgg
     2941 cggttttgca aacggctacg tgcgaaaagt tgaaacagct gctcaatcgc tggtgcccgc
     3001 gttaatcgag cctccaaaag acgatcacga ggcgctaaaa tccgaagaga cgtgggatgt
     3061 ttgggggtca tatcagcgcc gctcgcagga ggatggcgct ggcggtttaa tcgctccgcc
     3121 accgccacac caggacaacg gcaaatccgc agacgacaat caagtcttat tcgacttata
     3181 ctaaggaggt gcgcattgat gcgatgcttt gagagatacc gtttacatct aaatcgcctc
     3241 tcgctctcga atgcgatgat gcgcgtgata tcgagctgcg ccccaagctt gtgcggtgca
     3301 attgcatgga gcatttcctc atccggaccc gccgcagcgc aatctgcggg tggcggcact
     3361 gaccccgcca caatggttaa caatatatgc acgtttatcc ttggtccgtt cggccagtca
     3421 ctcgctgttc tcggcattgt cgctatcggg atctcctgga tgttcgggcg ggcttcgctt
     3481 gggctggttg ccggcgtcgt cggcggcatt gttatcatgt ttggggcgag cttcctcggc
     3541 caaacgctca ctggcggtag ttgatggctg atcgtttgga agaatcgacc ctttacctcg
     3601 cagccacacg gcccgcattg tttcttgggg tgccactgac attggcaggg ttattcatga
     3661 tgttcgccgg ctttgtcatc gttatcgttc agaacccgct ctacgaagtc gttctcgtgc
     3721 cgttatggtt tgcagcccgg ctcatcgtgg agcgagacta caatgcggcg agcgtcgtcc
     3781 tgctattttt gcggaccgcg ggaagaagca ttgatagtgc agtttggggg ggcgctactg
     3841 ttagcccaaa tccaattagg gttcccccac gagggagagg aatggtgtga tgctcggcgc
     3901 gagtggaacg accgaaagat ccggtgagat ctatctccct tatattggcc acctcagcga
     3961 ccatatcgtc cttcttgaag acggatcgat catgaccatt gcgagaattg atggcgttgc
     4021 attcgagctt gaggaaactg aaatgcgcaa tgcgcgttgt cgtgcgttca acacgctgtt
     4081 gcgcaatatc gctgatgatc atgtgtcaat atatgctcac ctcgtacgtc atgccgacgt
     4141 gccatcatcg gcgccgcgac acttccgtag tgttttcgcc gctagcctga acgaagcttt
     4201 tgaacagcgc gtgctctccg gccaactcct ccgcaatgaa cacttcctta cgttgattgt
     4261 ctacccacag gcggctttag ggaaggtaaa gaggaggttc accaagctaa gcggaaaaag
     4321 ggaaaacgat ctcacgggcc agatcaggaa catggaagat ctttggcatg ttgtcgctgg
     4381 ctctcttaaa gcgtatggcc tgcatcgtct tggcatccgc gagaagcagg gtgtgctctt
     4441 caccgaaatt ggcgaagcgc tacggttgat catgactggt cggttcacac cggttccggt
     4501 cgtcagcggc tcactcggcg cttcgattta taccgacaga gtcatttgcg gcaagcgagg
     4561 actcgagatc agaacgccaa aagacagtta cgttggatcc atctattcgt ttcgcgaata
     4621 ccctgcaaaa acacggccgg gcatgctcaa cgcgctgcta tccctcgatt ttccacttgt
     4681 tctcacgcag agtttttcgt tcctgactcg cccgcaagcg cacgcgaaac ttagcctcaa
     4741 atcgagccag atgctgagtt ccggcgataa agccgtgact caaatcggca aattatccga
     4801 ggctgaggac gcacttgcga gcaacgaatt cgttatgggc tcacatcatt tgagcctttg
     4861 cgtctatgca gacgatctca atagtcttgg ggacaggggc gcgcgggctc ggacacgaat
     4921 ggcggatgct ggagccgtgg ttgtccaaga aggtattggt atggaagcgg cctattggtc
     4981 ccaattgccg gggaatttta agtggcgcac acgccctggc gcaatcactt cacgcaattt
     5041 cgcagggttt gtctctttcg aaaactttcc agagggcgcc agctcaggcc actggggcaa
     5101 cgcgattgcc cgatttcgta ccaatggcgg aacgcctttc gactatatcc cgcatgagca
     5161 cgatgttggc atgacggcaa tattcgggcc tatcgggagg ggtaagacga cgctcatgat
     5221 gtttgttcta gccatgctcg aacagagcat ggtcgaccgt gcaggtacgg tcgtgttctt
     5281 tgacaaggac cggggtggcg aattgctggt tcgcgccaca ggaggaacat atttggcact
     5341 tcacagaggc acacccagcg ggttggcgcc gttgcgtggc ctagaaaaca cagcagcctc
     5401 acacgatttt ctgcgcgaat ggatcgtggc tctcatcgag agtgatggtc ggggtgggat
     5461 ttctccggaa gagaaccgcc gtctggtccg tggtatccat cgtcagctct cgtttgatcc
     5521 acaaatgcgt tcaatcgcgg ggttacgtga atttttgttg catgggcccg ccgaaggcgc
     5581 aggagcgcgg ctccaacgct ggtgccgggg ccatgcgctt ggctgggcat ttgacggcga
     5641 agttgacgaa gtaaagttag atccgtcgat taccggcttc gacatgacgc atcttctcga
     5701 atacgaggaa gtatgcgctc ccgctgcagc atatctcctg catcggattg gagccatgat
     5761 cgacggccgc cgttttgtga tgagctgcga tgagtttcgc gcctatttgt taaaccctaa
     5821 attttcgact gtcgtcgaca aattcctcct gaccgttcga aaaaacaacg ggatgctaat
     5881 actggcaacg cagcaaccag agcatgttct ggaatcgccg ctaggagcca gcttggttgc
     5941 gcaatgtatg acgaagattt tctatccatc accaaccgca gatcgatcgg cttatgtcga
     6001 tggactgaaa tgtaccgaaa aggaatttca ggcgatccgt gaagacatga cggtcggcag
     6061 ccgtaagttt cttcttaaac gagaaagtgg aagcgtcatc tgcgaatttg atctgcggga
     6121 tatgcgtgaa tatgtcgccg tgctttcggg gcgtgccaac acggtgcgct ttgcaactcg
     6181 actacgcgag gcacaagaag gcaactcatc tggctggctc agcgaattca tggcccgtca
     6241 ccacgaggca gaagattgat aaggtaggaa acgatgaaga cgacgcaact tattgcaaca
     6301 gttttgacct gcagctttct atatattcag cccgcgcggg cgcagtttgt tgttagcgac
     6361 ccggcaacgg aggctgagac gctcgcgact gcgctcgcga ctgcggagaa tctcactcag
     6421 actatagcga tggttacgat gttgacgtcg gcctacggcg ttactggact actgacttcg
     6481 ctcaaccaga aaaatcagta tccttcgacg aaggacctag acaatgaaat gttttcgccg
     6541 cgaatgccaa tgtcgaccac ggcacgtgcg atcaccagcg atacagatcg tgcagtcgtg
     6601 ggtagtgatg ctgaagcgga cctgttgcga tcgcagatca ccggttccgc aaacagcgct
     6661 ggcattgcgg ctgacaatct ggaaacgatg gacaaacgct tgacggcgaa tgctgatacg
     6721 tctgctcagc tttcccgatc tcgcaatatc atgcaggcaa ccgtgaccaa tggtttgctt
     6781 ctcaagcaga tccatgacgc aatgattcaa aatgtacagg cgacaagcct attaacgatg
     6841 actaccgcgc aggccggcct tcacgaggcg gaagaggcgg ccgctcaacg caaggagcat
     6901 caaaagaccg ctgtcatctt tggtgccctc ccctaaggct gggcgatttg ttcatccgcc
     6961 cgcatcctcg ccgaatgcga gctcatttta tccaacatta tgcgacaaac cagtcaagtt
     7021 caggtccaat cgatgaattt cacgattccg gcgccgttta cggccattca tacgatcttc
     7081 gatgtagcct tcacgacagg cttggactcg atgcttgaga ctatccagga ggcggtgagt
     7141 gcgccattga tcgcctgtgt cactctttgg attattgttc agggtatttt agtcatacgc
     7201 ggcgaagtcg atacccgtag cggtatcact cgggtgatca cggtcaccat cgttgttgct
     7261 ctaattgttg ggcaggctaa ctaccaagac tatgtggttt ccatcttcga aaagacggtc
     7321 ccaaactttg ttcagcagtt tagtgtaacc ggcttgcctc tgcagactgt tccggcacag
     7381 ttggatacaa tgttcgccgt gacccaggcc gtttttcaga aaatcgcatc cgaaatcggt
     7441 ccgatgaacg accaggacat ccttgctttc caaggggcac agtgggtcct ttacggcacg
     7501 ctctggtctg ccttcggagt ttacgacgcc gttggaattc tcacgaaagt gcttctcgcg
     7561 atcgggcctc tgatcctcgt cggatatatt tttgatcgca cgcgggacat cgcagctaag
     7621 tggatcgggc aacttatcac ctacggtctc ttgcttctcc tcttaaacct cgtggcaacg
     7681 atcgtcatcc taaccgaagc gactgcgctc acccttatgc ttggtgtaat caccttcgcc
     7741 ggtacgaccg cggccaagat cattggtctt tacgaactcg atatgttttt tctgacaggg
     7801 gatgcgctca ttgtcgcttt gccggcgatc gccggcaaca ttggaggcag ttactggagc
     7861 ggcgcaaccc aatctgccag cagcttgtac cgtcgcttcg ctcaggttga gcgaggctag
     7921 gtcgcgcaaa aattcgcctc aatggagaat tctatgaagt attgcctgct gtgcctagtt
     7981 gtcgctttga gcggctgcca gacaaacgac acattagcga gctgcaaagg cccgatcttc
     8041 ccgctgaatg tggggcgatg gcagcctact ccgtcagatc ttcagctcgg caattcgggt
     8101 ggacgctatg acggggcctg aatatgccat gctagtggcg cgcgaaagcc ttgccgagca
     8161 ctataaggaa gtagaagcct ttcaaaccgc gcgagcgaaa tcggcgcgac gtctctccaa
     8221 actcattgca gctgtcgcag ctatcgcgat tttgggaaat gttgctcaag cgttcgctat
     8281 agccacaatg gtgccgttga gcaggcttgt gcccgtatat ctatggatac ggccggacgg
     8341 caccgttgac agcgaggtgt ctgtctcgcg attgcctgca actcaagagg aggccgtcgt
     8401 taacgcctca ttgtgggagt acgttcgcct gcgcgagagt tatgatgccg acaccgctca
     8461 gtacgcctac gacctggtat cgaacttcag tgccccaaca gtgcgccagg attaccagca
     8521 attcttcaac tatcccaatc ccagttcgcc tcaagtcatt cttggcaaac gcggcagggt
     8581 ggaggtcgag cacatcgctt caaatgatgt aactccaagc acgcagcaaa ttcgctataa
     8641 aaggaccctc gtcgttgacg gcaaaatgcc tgtggtgagt acgtggaccg cgacagttcg
     8701 ctacgaaaag gtgaccagct tgcccggcag attgagacta accaacccgg caggtctggt
     8761 tgtcacctcc tatcagacat cggaagatac cgtttcaaac gtaggccaca gcgaaccatg
     8821 atcagaaaag cacttttcat tttagcatgt ttatttgccg ctgcgactgg tgcggaggct
     8881 gaagacactc caatggcggg caagctagat ccgcgcatgc gttatttggc ttacaatccc
     8941 gatcaagtgg tgcgcctctc gacggcggtt ggagctactt tggtcgtaac attcgccacg
     9001 aacgaaacgg tgacagcggt tgccgtttca aatagcaaag atctagcagc cctaccgcgg
     9061 ggaaattatc tatttttcaa ggcaagccag gtcctcacgc ctcagccagt aatcgtgcta
     9121 accgcaagcg actccgggat gcgccgttat gttttcagta taagttccaa gactctgtcc
     9181 cacctcgata aagagcagcc cgatctctat tacagcgtcc aattcgccta ccccgccgac
     9241 gatgcggcgg ctcggcgaag ggaggcacaa cagaaggctg ttgtggacag actacacgcg
     9301 gaagcacaat atcaacggaa agctgagaat ttattggatc agcctgtcac agcccttggt
     9361 gcggcggaca gtaattggca ctacgtcgcc caaggcgatc gttcgctgtt gccactcgaa
     9421 gtcttcgaca atggatttac gacggtattc cactttccgg gcaatgtacg cataccctcc
     9481 atctacacca tcaatcctga tggcaaggaa gctgttgcca actattcagt taaagggagc
     9541 gatgtcgaga tttcttcggt ttcccgaggt tggcgtctga gggatggcca cacagtacta
     9601 tgtatctgga acaccgctta cgatcccgtt ggccaaaggc cgcaaacggg cacggtgagg
     9661 cccgatgtga aacgcgtcct gaagggggcg aagggatgaa taacgatagt cagcaagcgg
     9721 cacatgaggt tgatgcatct ggatccctgg tctccgacaa acatcgccgg cgtctttcgg
     9781 ggtctcagaa attgatcgtc ggaggtgtcg ttctcgcgtt atcattaagc ctcatttggc
     9841 taggtgggcg ccaaaagaag gtgaatgaga acgcatcgcc gtcaactttg atcgcaacaa
     9901 acaccaagcc atttcatcca gctccgattg aggtgccgcc ggatcctcca gcggttcaag
     9961 aggctgttca gcctgctgct cctctaccgc cgaggggcga accggagcgg catgagccac
    10021 ggccggaaga aacaccgatt tttgcatata gcagcggcga tcaaggggtc agcaaacgcg
    10081 ccattcaggg cgacacgggc cgaagacaag aaggcaagcg tgacgacaac tccttgccga
    10141 atggcgaagt gtccggcgag aacgatttgt cgatacgtat gaaacccacc gagctgcagc
    10201 ccagcagcgc cacgctcttg ccgcaccccg attttatggt aacgcaaggg acaataattc
    10261 cgtgcatctt gcaaaccgca atcgacacaa atttggcagg ctatgtaaag tgtgtcttgc
    10321 ctcaggatat tcgtggaaca acgaacaata tcgtgcttct tgatcgtggc accaccgttg
    10381 ttggcgaaat acagcgtggc ttgcaacagg gagatgggcg cgtttttgtg ttgtgggatc
    10441 gcgccgagac acctgaccat gcgatgatct cgttaacatc gccaagcgcg gacgaactcg
    10501 gtcgctcagg attgccgggc tcggtcgaca gccacttctg gcagcgtttt agcggagcta
    10561 tgctcttgag tgttgttcaa ggcgccttcc aggcagctag cacctacgcc ggcagctcgg
    10621 gtggcgggat gagcttcaac agctttcaaa ataacggtga gcagacaact gagacagccc
    10681 ttaaggcaac catcaacata ccgccaaccc tgaagaagaa tcagggtgac accgtttcca
    10741 ttttcgtagc acgggacctc gatttctttg gtgtttacca gctccgcctg actggcggcg
    10801 ccacgcgggg gaggaaccgc cgctcttaat gaattcaaat ttccgcttag agataggata
    10861 cattgtaaat ggaagtggat ccgcaactac gctttcttct gaagccgatt ttggaatggc
    10921 tcgatgaccc gaagactgaa gaaattgcga tcaatcgacc tggagaggca tttgtgcgcc
    10981 aagccggcat ttttaccaag atgcctttgc ccgtctctta tgatgatctt gaagatatcg
    11041 ctattttagc gggcgcgctg agaaagcagg atgtcggacc acgtaacccc ctctgcgcca
    11101 ctgaacttcc tggtggtgaa cgactacaaa tctgtctgcc gccgaccgtt ccctcgggca
    11161 ccgtcagctt gaccattcga cggccaagct cgcgtgtttc tggtcttaaa gaagtctcct
    11221 cccgttatga tgcttcgagg tggaaccagt ggcagacacg aaggaaacgc caaaatcagg
    11281 atgatgaagc tatccttcag cattttgaca acggggattt ggaagcgttt ctgcacgcat
    11341 gcgtcgtcag ccgactgacg atgttgctat gtggccctac cggaagcggc aagacaacaa
    11401 tgagcaagac cttgatcagc gccatccccc cccaggaaag gctaatcacc atagaagata
    11461 cgctcgaact cgtcattcca cacgataatc atgttagact actctactcc aagaacggtg
    11521 ctgggctggg tgctgtgagc gccgagcact tgctccaagc aagtctgcgt atgcggccgg
    11581 accggatatt gcttggcgag atgcgcgacg atgcagcatg ggcttatctg agtgaagtcg
    11641 tctcgggaca tccgggatcg atttcaacaa tacacggcgc gaatccaatc caaggattca
    11701 agaaactgtt ttcccttgtc aaaagtagcg cccaaggtgc tagcttggaa gatcgcacac
    11761 tgattgacat gctctctacg gcgatcgatg tcatcattcc attccgtgcc tatgaggacg
    11821 tttatgaagt aggcgagatc tggctcgcgg cggacgcacg acgccggggc gagaccatag
    11881 gcgatctcct taatcaatag tagctgtaac ctcgaagcgt ttcacttgta acaacgattg
    11941 agaacttttg tcataaaatt gaaatacttg gttcgcattt tcgtcatccg cggtcagccg
    12001 caattctgac gaactgccca tttagctgga gatgattgta catccttcac gtgaaaattt
    12061 ctcaagcgct gtgaacaagg gttcagattt tagattgaaa ggtgagccgt tgaaacacgt
    12121 tcttcttatc gatgacgatg tcgctatgcg gcatcttatt atcgaatacc ttacgatcca
    12181 cgccttcaaa gtgaccgcgg tagccgacag cacccagttc actagagtac tctcttccgc
    12241 gacggtcgat gtcgtggttg ttgatctaaa tttaggtcgt gaagatgggc ttgagatcgt
    12301 tcgaaatctg gcggcaaagt ctgatattcc aatcataatt atcagtggcg accgccttga
    12361 ggagacggat aaagttgttg cactcgagct aggagcaagt gattttatcg ctaagccgtt
    12421 tagtacgaga gagtttcttg cacgcattcg ggttgccttg cgcgtgcgcc ccaacgttgt
    12481 ccgctccaaa gaccgacggt ctttttgttt tactgactgg acacttaatc tcaggcaacg
    12541 tcgcttgatg tccgaagctg gcggtgaggt gaaacttacg gctggagagt tcaatcttct
    12601 cctcgcgttt ttagagaaac cccgcgacgt tctatcgcgc gagcaacttc tcattgccag
    12661 tcgagtacgc gacgaggagg tttacgacag gagtatagat gttctcattt tgcggctgcg
    12721 ccgcaaactt gaggcggatc cgtcaagccc tcaactgata aaaacagcaa gaggtgccgg
    12781 ttatttcttt gacgcggacg tgcaggtttc gcacgggggg acgatggcag cctgagccaa
    12841 ttgcatttgg ctcttaatta tctggctcaa aaggtgactg aggacgcggc cagcggcctc
    12901 aaacctacac tcaatatttg gtgaggggtt ccgataggtc cctcttcacc aattgctcga
    12961 tggcttctct ccagcaaaga atgacgcgag cgcggcggta gccagcttgt ggccgaaagc
    13021 tcgagcggtc tccaacccca acggatcaaa atgacttcga gcgacctcga gcaacgcaac
    13081 cgggaacatg cgtgaggtct gaacgagaac ggatttttct gtagttgaag ggatcggata
    13141 acttttcggg gccacgcgaa atgatccatc tgccagcatg ctttcgaaat cgtccaacgc
    13201 gcgccttaaa atcatttgta gcgacttcga gggactgtat tgccgaacga ggttgtcata
    13261 tgttttcgac acttgaggcg cgggcggtcg cgctgaaaga aaaacctgga gctttttcgg
    13321 ggacggaggt ggactaaggg catccacagt tagcttaagt tgtcgatcgg gactgtaaat
    13381 gtgatcggcg acgagaggct cacgttgctg gtctttctcg tcggcttttt caggcaagtg
    13441 ctggaggtcc agcttctggg gaacaagtgt cgggttggga tggtggatct cgggtcgagc
    13501 accagcaagc cgccgtgctt cgccgaccga caatgcgggc ttgcgaattg ccatcttcaa
    13561 gcctccaaga ttttgctgat cagtttcgaa atgaccacga cttcctccat cgcaatccga
    13621 agattcctct ctatgaggcg catcgtcgga tcagttcccg tgtttagtaa tgtaagatgc
    13681 aacatgccgc gttctttcat cgcggcaaat gcatctcttt catgcatggg agacggtaca
    13741 actggaaggc tctctagcgt ctctgacatc ctgcgttgcg atgttgtcaa tcggccgacc
    13801 gggacgcgtt ggcgcaaaac agctgtagga attgccaaat tttcactcaa cagcagctcg
    13861 atgacgtagc ggtaggtaga tagtgcctca tcgatgtcga gcggcgttag catggtgggg
    13921 atcagaagca ggtttgagct agcgatgatt gtgttgttga gctcgctcga gccgccacgc
    13981 gtatcggcca acgcataatc aaatccttcg agctcggcat tttcataggc tgcttcaaga
    14041 aggggcattt cgtcggcgga atagacttca cagcgaggat cccaggtact gctttgtaag
    14101 gcgttttctc tccatcgcgt cagaggccgg ttttcgtcgg catcaaagag ggccactcgt
    14161 ttaccgtcat ttgccaaagc agcgcaaagg cccatgagtg cggtggtttt gccagcaccc
    14221 cctttgaaag aacaaaacgt caaaagttgc atattctgat cccgcctatc ctgtgaaacc
    14281 ggagtgcatt tgtatttttg ttcgtataaa tgtttttgtg attatcgatg agtaaaagcg
    14341 ttgttacact atttttattt cacattcgtt ataagacaat tgcaaatgta gcaagtatat
    14401 tcagtattga ctgtaaatgt actgttgatt tcatattgag cagggctaga cttccatccg
    14461 tctacccggg cacatttcgt gctggagtat ccagaccttc cgctttcttt ggaggaagct
    14521 atgtcaaaac acaccagagc cacgtcgagt gagactacca tcaaccagca tcgatccctg
    14581 aaagttgaag ggttcaaggt cgtgagtgcc cgtctgcgat cggccgagta tgaaaccttt
    14641 tcctatcaag cgcgcctgct gggactttcg gatagtatgg caattcgcgt tgcggtgcgc
    14701 cgcatcgggg gctttctcga aatagatgca gacacacgag aaaagatgga agccatactt
    14761 cagtccatcg gaatactctc aagcaatgta tccatgcttc tatctgccta cgccgaagac
    14821 cctcgatcgg atctggaggc tgtgcgagat gaacgtattg cttttggcga ggctttcgcc
    14881 gccctcgatg gactactgcg ctccattttg tccgtatccc ggcgacggat cgacggtcgc
    14941 tcgttactga aaggtgcctt gtagcacttg accacgcacc tgacgggaga aaattggatg
    15001 cccgatcgcg ctcaagtaat cattcgcatt gtgccaggag gtggaaccaa gacccttcag
    15061 cagataatca atcagctgga gtacctgtcc cgaaagggaa agctggaact gcagcgttca
    15121 gcccggcatc tcgatattcc cgttccgccg gatcaaatcc gtgagcttgc ccaaagctgg
    15181 gttacggagg ccgggattta tgacgaaagt cagtcagacg atgacaggca acaagactta
    15241 acaacacaca ttattgtaag cttccccgca ggtaccgacc aaaccgcagc ttatgaagca
    15301 agccgggaat gggcagccga gatgtttggg tcaggatacg ggggtggccg ctataactat
    15361 ctgacagcct accacgtcga ccgcgatcat ccacatttac atgtcgtggt caatcgtcgg
    15421 gaacttctgg ggcaggggtg gctgaaaata tccaggcgcc atccccagct gaattatgac
    15481 ggcttacgga aaaagatggc agagatttca cttcgtcacg gcatagtcct ggatgcgact
    15541 tcgcgagcag aaaggggaat agcagagcga ccaatcacat atgctgaata tcgacgcctt
    15601 gagcggatgc aggctcaaaa gattcaattc gaagatacag attttgatga gacctcgcct
    15661 gaggaagatc gtcgggacct cagtcaatcg ttcgatccat ttcgatcgga cgcatctgcc
    15721 ggcgaaccgg accgtgcaac ccgacatgac aaacaaccgc ttgaaccgca cgcccgtttc
    15781 caggagcccg ccggctccag catcaaagcc gacgcacgga tccgcgtacc attggagagc
    15841 gagcggggtg cccaaccatc cgcgtccaaa atccctgtaa ctgggcattt cgggattgag
    15901 acttcgtatg tcgctgaagc cagcgtgccc aaacaaagcg gcaattccga tacttctcgc
    15961 ccggtgactg acgttgccat gcacacagtc gagcgccagc agcgatcaaa acgacgtcat
    16021 gacgaggagg caggtccgag cggagcaaac cgtaaaagat tgaaggccgc gcaagttgat
    16081 tccgaggcaa atgtcggtga gcccgacggt cgcgatgaca gcaacaaggc ggctgatccg
    16141 gtgtctgctt ccatccgtac cgagcaaccg gaagcttctc caacgtgtcc gcgtgaccgt
    16201 cacgatggag aattgggaga acgcaaacgt gcaagaggta atcgtcgcga cgatgggcgc
    16261 ggggggacct agagacagga aggaccgaat aatggcaaat ggtcagttca cgatacgctc
    16321 tgctcgcccg gcctccgtcg gactgacagg cgaacggcgt ggagccgcat ccgcctctag
    16381 ctctgcactg tccaatgttc aaagagatgt tagggatagg ctgattccaa ctagctcacc
    16441 aagattacca aatgcagcca tattgcgtga ttcctcggga agagcgtcga ctggtctgcg
    16501 gtacatggcg gctactcttc attggtctgc gatcgcgcca ttatcgctaa taaacagcaa
    16561 cgacctggct ccggccgctt atgactttga gacgcgaaat aacgcaagaa atgtgactgc
    16621 caaagtcggc agggcagtcc ctgttcccaa gcaaggcggg ctcggcaaaa cgctcgcacc
    16681 cgtacccctt agtacacgta tatcaagggt caattccgac cgaagactgc ccgctgacgc
    16741 agaagaccgc cctgaaacgc gcgaccccca gaaaggacgt ggcagtcatg gtgcgacgcc
    16801 aaccttacat gaaaagattg gaaccgcgtt tgctcgaaga ttgcgaaagc atacgtacta
    16861 tattgtttgc agttgctgcc agacccggag cgcgttgacg atgggtgcaa agatttcggt
    16921 gaagtcatga actccagcaa gacttcgccc cagcgtatga ccctgagcat cgtatgttcg
    16981 ctggcagccg gtttttgtgc ggccagctgc tatgtaacgt tccgccgggg cttcaacggc
    17041 gaagcgatga tgacgttcga cgttttcgct ttttggtatg agaccccgct ttacttgggt
    17101 tatgccagca ccgtcttctg gcgtggttta tctgttgtca tctttacctc gctgatcgtt
    17161 ctttcaagtc agctcatcat atcgctgcgc aatcagaagc atcatgggac agctcgttgg
    17221 gcagaaattg gcgaaatgcg gcatgctggt tatctgcagc gttacagtcg catcaagggg
    17281 ccgatctttg gaaagacatg tggtcccctt tggttcggca gttatttgac caatggcgaa
    17341 cagccacaca gtcttgtcgt cgcgccaacg cgtgctggca aaggcgtcgg catcgtcatt
    17401 ccaacgctgt tgaccttcaa gggctcggta atcgcccttg acgtcaaggg agaattgttt
    17461 gaactgacgt ccagagcacg caaagcgagc ggcgacgcag ttttcaagtt ctccccccta
    17521 gatcctgagc ggaagactca ttgttacaat ccggtcctgg atattgccgc acttccgccc
    17581 gaacgccagt tcactgaaac acgccgtcta gctgcgaacc ttattacggc taagggaaag
    17641 ggagcagaag gctttattga cggcgcacgt gacctgttcg tcgcgggaat ccttacctgc
    17701 attgagcgtg gcacaccaac gattggcgcg gtatatgacc tatttgcgca gcctggcgaa
    17761 aagtataagc tttttgcgca actcgcggag gaaagcctaa acaaagaggc tcagcgtatc
    17821 ttcgataata tggcgggcaa cgacacgaaa attctgacat cgtacacctc tgtgctgggc
    17881 gacggtggac tgaacctgtg ggctgatccg cttatcaaag cagcgacaag ccggtcagac
    17941 ttttccgttt acgatctccg gaggaagaag acctgcattt atctttgtgt cagtcccaac
    18001 gatctggagg tcttggcacc acttatgcgc ctgatgtttc agcagctcgt gtcaatcttg
    18061 cagagatcgc tgccaggtga agacgagtgc catgaagttt tatttctcct cgacgaattc
    18121 aaacacctgg gcaagcttga ggccatagag accgcgatca caaccatcgc cggttacaga
    18181 ggccgcttta tgtttattat tcaaagtctt tcggccttgt cgggcacata cgatgacgca
    18241 ggaaaacaaa actttctgag caatactggc gtacaagtat ttatggccac ggctgatgac
    18301 gaaactccaa cctacatctc aaaagctatc ggcgaatata cgtttaaagc gcgttcgacc
    18361 tcttacagtc aagccagaat gttcgaccac aacatccaga tttctgatca aggtgcagcc
    18421 cttttgcgcc ccgaacaagt gcgcctgcta gacgatcaga gtgaaatcgt tctcatcaaa
    18481 gggcgacctc cactcaaatt acgaaaggtg cagtattatt ccgatcgtac gctgaaaggc
    18541 cttttcgaac gccagatggg ctctctgcct gagcccgcac ccttgatgct ttccgactat
    18601 agcaacgatc aagttcaata ccacttggct ccgatagcaa attttaatga ggatgctgca
    18661 ccgcaaaaca gaactgtggc cgaggaccat ggaagtgtta aagtcggtgc tgatatccct
    18721 gaacgcgtga tgggaataaa tggtgacgag gaacaagccg atgcgggcga gataccgccg
    18781 gaatcggttg tgcctccaga attgacgctc gctctgaccg ctcaacagca attgttggac
    18841 cagattattg cacttcagca aagatcgagg tccgcaccgg catagcctgc gaaatgatct
    18901 taatggtgca attcgtttca ggcggcatgt tgcggttcaa caaagtgtac gccagatccc
    18961 aattggccct ttacgaggtg tcggtatgac aggaaagtcg aaagttcaca taagaggttc
    19021 ggctgacgcg cttcctgacg ttcctggcgg aagtactacc gccccttttt taaccgaacc
    19081 ttctcgggat caggttgatg cctcgtttga ggtccaaacc gactacagcc agtctacttc
    19141 cgtgtcgttt acctatgatg gtgttggact tggtcctgcc gagcgtgcgg cttacgagaa
    19201 ctggtgcgaa ccgggccggc ccacttggaa agatcttata atcaaggcac gtgtcgatcc
    19261 gattgacgat gtgacctggc tccgagattt agaagaggac accccctcaa ccttcagata
    19321 cgaagggatg cctctgggca tcggggaacg acaggcctac gaaaattggc aagaggacgc
    19381 tcagccgaca tgggaagacc ttgttgtcag cgcacgcttg acggaacttg gccgtccaca
    19441 cgggattacc ggcgagtata catccctcgc aggatcgaag aatacaagtt caatttcatt
    19501 gaagcgaaag cggagcaact taattgatga tgagaattca tccggatcgt tttcatatga
    19561 cgggatgaag ctcggggaag ccgagcgttc tgcatatggt gactgggccg aggcggagcc
    19621 acccacgtgg aaagatttgg tattgagggc acgcgtttcc tcgatcaatg actctgcttg
    19681 gctttttgat tcacaaacat cttcatcatc atttgaatac aacggtgttc ccttgggcga
    19741 gccggaacgg caggctctca gacaatggca aggagacgct cagcctacct gggaagatct
    19801 cgttgttaac gcgcgtatgg cagaactttg ccatgctggt tggattgaag gtcaaaaagg
    19861 ttgctttgaa gagcgcgggg aggctctgcc cgcgtcggaa cgcggttcgc aacgccccat
    19921 tggtcaacgg acagattcct ccgattcttt tgtgtatgat ggcacaaggc tcggagcacc
    19981 tgagcgaact gcttatgaac gctggagtaa gagggaacgc ccgacttggg aagatctcat
    20041 cttagatgca caccaggcca ggactgaaag tgacgctgtt acgacccaag cgattggtca
    20101 gtcgtcctca ccggttttct tatatgaagg aaagtcgctc ggagacaggg aacgaaaggc
    20161 ttacgaaaaa tggcggcagc cagcccaacc gcgatggcaa aatcttgtag tgaacgctcg
    20221 tctggcagaa atcgatccct cagcctggat tgccgatgag cgcgatccgc ttgatgatag
    20281 cgacgcgctt ggtcgcccgt cgtacacaag cttgacggat agatcagacg tccctttaga
    20341 cgatcaatca atctatcgtc gttccgacct agtaagggaa caagtaccag aatcgtctca
    20401 aaggcaattc gcagcatgtt cagaatctga aacgaggcct gtgcaatggt ttactgcttc
    20461 tgggtcagat gcaaacaata cggaaaatat caccgccagc gatcccgtcg atcgcacggg
    20521 tggagttaag cggctaggct ccaaaagcga cagaaccgtt acagcttcta tccatgacgt
    20581 gaattccagc acaaggcgac tgttgcttaa cgaatttgga tcggaggctc cgcgcccttc
    20641 gccagaaaag actgttcgct taagaagcga caatattggc acctatggga gccggaaaaa
    20701 tgaacgagcg cggctcgcga ccgaaaccgg tgcgtatgag tcggagcata ttttcgggtt
    20761 caaggctgtc cacgatactg cgagagcgac gaaagagggc cggcgtctcg aaaggcccat
    20821 gcccgcctac cttgaggata aggggcttca tcgccaacat attggcaccg ggagaggacg
    20881 gaccaaactt gtcgggcgcg gatggccgga tgacacaagc tatcgctcgg atcaaagggc
    20941 aactctgtcg gaccccgttg cgcgctcgga aggcgcgacg gcctcaaatg ggtatcaatt
    21001 gaaccaattg ggttacgcgc accaactcgc tagcgatggc ctgcaaagtg aatcgcccga
    21061 tggtgttgcc ttgccaattc aagtggcaac aacgagctac aactatacag tgagccgcga
    21121 tcctgtcctt gttccgccgg ataaaaacga agcccctcaa ttgctgcatc ttggtccccg
    21181 tggtcaaacc gaagctgttc ttgcccgcga aacagcattg actggaaaat ggccgactct
    21241 cgagcgtgag cagcaagtgt atcgcgagtt tttggcctta tatgacgtaa aaaaagatct
    21301 tgaggccaaa tcagtcggcg taagacggaa aaaaaaagaa gttatttctg cgttagaccg
    21361 aactgcgcgc ttgataagca cgtcgccttc gaaagctcga tccaaagcag agactgaaaa
    21421 agccattgat gagctcgatg atcgacgagt ttatgatccg cgtgatcgag ctcaagacaa
    21481 agcgtttaaa cgctgataag tcgccaatat agtgatcatt tgcagtattc gcatcgatcg
    21541 ctggttgata ttctgccgct ggtcgaccgg ctgctcgtcg ccaaaaatgc tcacagggat
    21601 acgatggcct cggtcaggcc gcgtgcgtcc tgtctttcca gttcctccct ttcagctcga
    21661 ttgtggcatc atttattgcc tgctcattgc agttgaaacg cgatatccgt ttcaagaccc
    21721 gggtatggat ggtactttgg agatatgagc acttgccaga cttcccggtc ggagacttaa
    21781 aaattccgat aagaggttac ggacgacgga ccttataagt ggatcgtcta gtggtggcgc
    21841 cgatcaaaac agttccgccc ccggctttgc tcaaagtagc aaagcagctt tatctcgggt
    21901 tgcggaggat tttctagaaa accgcaattt tgcaggagag aacatatggc catcatcaag
    21961 ccgcatgtga acaaaaatag gacaacctcg ccgatagaac gtccggagtc tctcatagag
    22021 gaaatgagcg gcagtcatcc gccgagtggt tttaccaacc tggatctcgc tatgatcgag
    22081 ctggaggact ttgtccatcg gtgcccgctc ccagaagaca atcttgctgg tcagaaggag
    22141 tgagacgatg gatccgtcta gcaatgagaa tgtctatgtg ggtcgcggtc acaacatcga
    22201 aaatgatgat gacactgacc ccaggcgttg gaagaaggcg aatatcagtt ccaacaccat
    22261 ctccgatatt cagatgacga atggcgaaga cgtacaatca gggagcccta cccgaacgga
    22321 agttgtaagc ccacgtctgg attatggatc ggtcgactcc tcctccagcc tttattctgg
    22381 cagcgagcac ggaaatcaag ctgagattca aaaagagctg tccgtcttgt tctcgaacat
    22441 gtctttgcca ggcaacgatc ggcgcccgga cgaatacatt ctcgtgcatc aaacgggaca
    22501 agatgctttt actggtattg ccaaaggcaa cctcgaccaa atgcccacca aggcggaatt
    22561 taacgcgtgc tgccgtctct acagggacgg agccggtaat tactacccgc cacctctcgc
    22621 attcgacaag attagcgttc cagagcaact ggaggaaaaa tgggggatga tggaggcgaa
    22681 ggaacgtaac aaactgcggt ttcagtacaa gttggacgta tggaatcatg cgcacgctga
    22741 tatggggatc acgggcacag agatctttta tcaaacagat aagaacataa agctcgaccg
    22801 gaattataaa ctaagacctg aagaccgata cgtacaaaca gaaaaatacg ggcgccggga
    22861 aattcaaaag cgatatcaac acgaactcca ggctggttcg ctgctgcccg atattatgat
    22921 caaaactccc caaaatgaca tccacttcgt gtacaggttt gccggcgaca attacgccaa
    22981 caaacagttc agcgagtttg aacacaccgt caagcgcagg tatggcgacg agactgagat
    23041 caaattgaag tcaaagtcag gcattatgca tgactcgaaa tatctggaat cctgggaacg
    23101 gggcagtgcg gatattcgct tcgcggaatt cgttggggaa aatagagctc acaatcggca
    23161 gtttccaact gcgacagtaa atatgggaca gcagccagac gggcagggcg gtttgacccg
    23221 cgaccgtcat gtgagcgttg acttcctaat gcaaagcgca cccaattcgc cttgggcgca
    23281 agctttgaaa aagggagaac tgtgggatcg cgttcagttg cttgctcgcg acggcaaccg
    23341 ctatctgtcg ccgcccagat tggaatattc tgaccctgca catttcaccg agttgatgaa
    23401 ccgggttggt ttacccgcat cgatgggtcg gcaaagccat gcggctagta tcaaattcga
    23461 aaagtttgac gcgcaggcag cggttattgt cttaaatggc ccagagttac gtgacattca
    23521 tgacttgtct cctgaaaaac tgcaaaattt gtccaccaaa gatgtcatcg tcgccgatcg
    23581 caatgagaat ggtcagagaa ctggcacgta caccagcgtc gcggaatatg agcgcttgca
    23641 gttaaggctg ccacccgatg cagcgggggt gcttggtgaa gcaactgaca aatattcacg
    23701 tgatttcgtt cggccagagc cggcgtcgcg tccaatcagt gacagccgca ggatatacga
    23761 aagtcgaccg cgtagccaaa gcgtcaacag cttttgacgt tcctgctgcc gcgtcaacga
    23821 ggaagctcgt ttgacccggg tttgccaatg aaagggctca atcatggtga acactacaaa
    23881 gaaaagtttt gcgaagtcgc ttacggcaga tatgcgccgt tctgctcagc gcgttgtcga
    23941 gcaaatgcga aaagcattga ttaccgaaga agaggcgctc aagcggcaag ccagactgga
    24001 gagtcccgat aggaagcgaa agtatgctgc tgatatggcg atagtcgaca aactcgacgt
    24061 agggtttcga ggcgaaatag gctataaaat tcttggaaat aaccggcttc gagtagacaa
    24121 ccataaagaa ttaacgcgtg agcacggtag acttcgcaaa accaaaacgg ttctgaagcg
    24181 taacccggtg acgcaggaag tctatttggg tttatatgaa aggaagtcct ggttaagtgt
    24241 cagcagccat ttgtatgctg cggacggcac actccgcatg aagcacgtga aatacaaaga
    24301 cggacgtttt gaggaaaaat gggagcgcga cgaaaatggc gacctgatcc gcacaaggta
    24361 cgccaaccgt ggcaggctct ttcaacctgt atccgagaaa atgggcgcgc cgtatcggag
    24421 cggccctgac gaccggctct atcgcgatct aacccgtcga aacggtttca gacgggagac
    24481 attcgaacgg gacgatcacg gaaacctcga gcgtatcggc agcaaccatg tcggcttttc
    24541 caagatttca gtgaaggcac ccaatcgtca aacctcccag acgaagattc aaaaacttgg
    24601 tggcgctttc aacaaatctt ttaggtccct tctggacaag gagggcaatg aaatgggccg
    24661 cgatattttg agccatcgac ggctctataa caagcggtct gctgtctacg atgaagctac
    24721 cggacaattg aagagtgcca agcatacctt cggcaagatc tacaggagcg aaaccgaata
    24781 tctcagcgcg ggcctcaaga aggtttcaaa aaagatactc ggggtgacgg tctaccggaa
    24841 atttgcggcg ctcagcgagc gagaatccga ggctgagaga ctgcgtagtt ttgaatccgg
    24901 tgcgcatcgc cagatctggc aggagcgggc agcgattccc ggttcgcccc tcccggagac
    24961 tgatgacatt catttcgcac agcagtcgca cctagccaaa gccaaccctg atcacgtcga
    25021 agctgacgtc acgcgtgtga cagatcaaca tgttgatgtt gctggacaaa catcatcgtc
    25081 tccccaacgg aacttggaag gatggttaga ttctcaatca cgatacaagc cagcaaacat
    25141 gctgttgtca aatccagacc ttcaagcgaa cggacctcgc ccatacgaag ggttagctca
    25201 tctcaccctc cggcgcgata atgaatctga cgggcacaag gagaatgatc agcggctgcg
    25261 acatttctcc cagccagagc cgttggtgtt accgcatccc gggtcgccgg aaataactaa
    25321 ggtgtttggc tcgcggggag agccggcaca cccgagtgga aatctgcaca cggcggttgg
    25381 agaaacggct tgcgaaggac cggtgatgtc ttcatcctcg gacaatcatc agccagctcc
    25441 aggacagcaa gaacttttaa gtttccttca taatgcgcca gccccagttt ctgtggcaat
    25501 acatgatgat caagagcgac ttgcggggga ggcgcccggc ggctctttca gaggtagctc
    25561 agggcgaacg agttcaatgt cggagagtat cttcgacgaa gatgtacaag ggcatttggt
    25621 acgggattat tcgatcaata ctactaacgg gtttattgac ccgcaatcgt tgttcggtga
    25681 accggactta tcgagaggtc caaaatcggg gccagaaatt ccatcggaag attaccattt
    25741 gtcagcttcg gaacaggaaa atttgctgaa tcaattgctt agtgtgccac tgccggttcc
    25801 ttcaccgaag cccgaatgcg cgaggtctat gattttcgaa ggttcacgtt caagagagcg
    25861 ttccacctcc agagggttct aaggtggaac gcgtctaggc ttgcctatca tcaccacctc
    25921 atgatggtcg cggcttaggg ataaccggtc ttgtccacta ccttgcagta atgcggtgga
    25981 caggatcggc ggttttcttt tctcttctca acctaggcta cagccgatag tctggaacag
    26041 cgcacttacg ggacctaggg cgatgcaggt ggctgctgaa cccccagccg gaactgaccc
    26101 cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag
    26161 gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg
    26221 ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg
    26281 gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc
    26341 ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag
    26401 gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga
    26461 caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg
    26521 cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg
    26581 gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc
    26641 caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt
    26701 gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat
    26761 tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat
    26821 cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt
    26881 cgtgtgtttc agcaacgcgg cctgcttggc ttcgctgacc tgttttgcca ggtcctcgcc
    26941 ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc
    27001 caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg
    27061 cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac
    27121 tatcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat
    27181 ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg
    27241 gtcaaacgtc cgattcattc accctccttg cgggattgcc ccggaattaa ttccccggat
    27301 cgatccgtcg atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
    27361 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
    27421 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
    27481 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
    27541 cggggtcagc accgtttctg cggactggct ttctacgtgg ctgccatttt tggggtgagg
    27601 ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg
    27661 gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca
    27721 gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta
    27781 gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag
    27841 cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa
    27901 ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact
    27961 tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc
    28021 gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt
    28081 caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt
    28141 gagggccaag ttttccgcga ggtatccaca acgccggcgg ccctacatgg ctctgctgta
    28201 gtgagtgggt tgcgctccgg cagcggtcct gatcccccgc agaaaaaaag gatctcaaga
    28261 agatcctttg atcttttcta cactagtgct tggattctca ccaataaaaa acgcccggcg
    28321 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
    28381 caggagtcca agcgagctca ccttcgggag cgcctgaagc ccgttctgga cgccctgggg
    28441 ccgttgaatc gggatatgca ggccaaggcc gccgcgatca tcaaggccgt gggcgaaaag
    28501 ctgctgacgg aacagcggga agtccagcgc cagaaacagg cccagcgcca gcaggaacgc
    28561 gggcgcgcac atttccccga aaagtgccac ctgcggcgtt gtgacaattt accgaacaac
    28621 tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg
    28681 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga
    28741 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc
    28801 ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctctcc ggagactgcg
    28861 agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg
    28921 agagcgccaa caaccacttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
    28981 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
    29041 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
    29101 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
    29161 tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
    29221 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca
    29281 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
    29341 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc
    29401 ttatgtcaac tgggttcgtg ccttcatccg tttccacggt gtgcgtccat gggcaaatat
    29461 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
    29521 gatggcttcc atggcttccc aaccttacca gagggcgccc cagctggcaa ttccgacgtc
    29581 ctcggtggtt ggcatgcaca tacaa
//

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