| 质粒名称: | pTA7002-avrPto |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 49156 |
| 存储实验室: | Sheng Yang He |
|
载体骨架基本信息 |
|
| 载体名称: | pTA7002 |
| 载体出品公司: | Nam-Hai Chua |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物转化双运载体 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | avrPto,avrPto1,avrPto1DC3000 |
| 物种来源: | Pseudomonas syringae pv. tomato DC3000 |
| 插入大小: | 495 bp |
| 基因突变: | -- |
| GenBank编号 | NC_004578.1 |
| 启动子 | 地塞米松诱导 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI(未破坏) |
| 3' 克隆位点: | SpeI(销毁时未知 ) |
| 5' 测序引物 | CCGCTCGAGACCATGGGAAATATATGTGTC |
| 3' 测序引物 | GACTAGTTCATTGCCAGTTACGGTACG |
|
文献引用方法 |
|
| 材料和方法部分: | pTA7002-avrPto was a gift from Sheng Yang He (Addgene plasmid # 49156 ; http://n2t.net/addgene:49156 ; RRID:Addgene_49156) |
| 参考文献部分: | A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants. Hauck P, Thilmony R, He SY. Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8577-82. Epub 2003 Jun 19. 10.1073/pnas.1431173100 PubMed 12817082 |
买家导航
pTA7002avrPto载体基本信息
pTA7002avrPto载体图谱质粒图谱和多克隆位点信息
pTA7002avrPto载体简介
pTA7002avrPto载体序列
Snapgene格式：pTA7002-avrPto质粒序列 Genbank格式：pTA7002-avrPto质粒序列
LOCUS pTA7002-avrPto 14787 bp DNA circular SYN 18-JUL-2024
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14787)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 14787)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 18, 2024 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..14787
/mol_type="other DNA"
/note="color: #ffffff"
/organism="synthetic DNA construct"
CDS complement(148..516)
/codon_start=1
/label=traJ
/note="oriT-recognizing protein"
/note="color: #993366"
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT complement(549..658)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
/note="color: #a6acb3"
CDS complement(1342..1989)
/codon_start=1
/label=TetR
/note="tetracycline resistance regulatory protein"
/note="color: #993366"
/translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
misc_feature complement(2454..2478)
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
/note="color: #a6acb3; direction: LEFT"
promoter 2993..3338
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
/note="color: #ffffff"
CDS 3600..3833
/codon_start=1
/label=VP16 AD
/note="transcriptional activation domain of herpes simplex
virus protein VP16 (Triezenberg et al., 1988; Cousens et
al., 1989)"
/note="color: #993366"
/translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
rep_origin complement(4164..4788)
/direction=LEFT
/label=oriV
/note="incP origin of replication"
/note="color: #ffff00"
promoter 5158..5341
/label=NOS promoter
/note="nopaline synthase promoter"
/note="color: #ffffff"
CDS 5394..6404
/codon_start=1
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
/note="color: #993366"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRP"
terminator 6457..6709
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
/note="color: #ffffff"
promoter 6929..6975
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
/note="color: #ffffff"
CDS 7010..7504
/label=avrPto
/note="color: #993366; direction: RIGHT"
misc_feature complement(8651..8675)
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
/note="color: #a6acb3; direction: LEFT"
CDS complement(10274..11419)
/codon_start=1
/label=trfA
/note="trans-acting replication protein that binds to and
activates oriV"
/note="color: #993366"
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
CDS complement(11721..12512)
/codon_start=1
/label=KanR
/note="aminoglycoside phosphotransferase"
/note="color: #993366"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
mobile_element 12704..13471
/label=IS1
/note="prokaryotic transposable element"
/note="color: #a6acb3"
rep_origin complement(14172..14782)
/direction=LEFT
/label=oriV
/note="origin of replication for the bacterial F plasmid"
/note="color: #ffff00"
ORIGIN
1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
2461 cgccaatata tcctgtcaaa cactgatagt ttcctgcagg tccccagatt agccttttca
2521 atttcagaaa gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca
2581 agacgatcta cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag
2641 atgcagtcaa aagattcagg actaactgca tcaagaacac agagaaagat atatttctca
2701 agatcagaag tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag
2761 taatagagat tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa
2821 agattcaaat agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga
2881 gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac
2941 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt
3001 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact
3061 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
3121 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
3181 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
3241 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
3301 cctctatata aggaagttca tttcatttgg agagaacacg ggggactcta gtttaaacaa
3361 tggcagatcc aatgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta
3421 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg
3481 agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat ctgacagaag
3541 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct aggtcgagcg
3601 cccccccgac cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtggcga
3661 tggcgcatgc cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc
3721 cgggtccggg atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact
3781 tcgagtttga gcagatgttt accgatgccc ttggaattga cgagtacggt ggggatccaa
3841 ttcagcaagc cactgcagga gtctcacaag acacttcgga aaatcctaac aaaacaatag
3901 ttcctgctgc attaccacag ctcaccccta ccttggtgtc actgctggag gtgattgaac
3961 ccgaggtgtt gtatgcagga tatgatagct ctgttccaga ttcagcatgg agaattatga
4021 ccacactcaa catgttaggt gggcgtcaag tgattgcagc agtgaaatgg gcaaaggcga
4081 taccaggctt cagaaactta cacctggatg accaaatgac cctgctacag tactcatgga
4141 tgtttctcat ggcatttgcc ctgggttgga gatcatacag acaatcaagt ggaaacctgc
4201 tctgctttgc tcctgatctg attattaatg agcagagaat gtctctaccc tgcatgtatg
4261 accaatgtaa acacatgctg tttgtctcct ctgaattaca aagattgcag gtatcctatg
4321 aagagtatct ctgtatgaaa accttactgc ttctctcctc agttcctaag gaaggtctga
4381 agagccaaga gttatttgat gagattcgaa tgacttatat caaagagcta ggaaaagcca
4441 tcgtcaaaag ggaagggaac tccagtcaga actggcaacg gttttaccaa ctgacaaagc
4501 ttctggactc catgcatgag gtggttgaga atctccttac ctactgcttc cagacatttt
4561 tggataagac catgagtatt gaattcccag agatgttagc tgaaatcatc actaatcaga
4621 taccaaaata ttcaaatgga aatatcaaaa agcttctgtt tcatcaaaaa tgactcgacc
4681 taactgagta agctagcttg ttcgagtatt atggcattgg gaaaactgtt tttcttgtac
4741 catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa
4801 atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta
4861 atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata agagatatgc
4921 aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata
4981 tgaggagtaa aacactagat ccccaaacaa gcttgaaact gaaggcggga aacgacaatc
5041 tgatcatgag cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa
5101 gccgttttac gtttggaact gacagaaccg caacgattga aggagccact cagccgcggg
5161 tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt
5221 cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc
5281 ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaatcca aataatctgc
5341 accggatccg ctagaggatc tcgaccctgc aagatcccgg ggggcaatga gatatgaaaa
5401 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
5461 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
5521 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
5581 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
5641 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
5701 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
5761 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
5821 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
5881 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
5941 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
6001 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
6061 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
6121 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
6181 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
6241 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
6301 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
6361 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgggatct tggaggtgat
6421 gtaacatgat cacaagctga tcccccgaat ttccccgatc gttcaaacat ttggcaataa
6481 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
6541 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
6601 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
6661 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattgatc
6721 ccccctcgac agcttgcatg ccggtcgact ttagaggatc cgggtgacag ccctccgacg
6781 ggtgacagcc ctccgacggg tgacagccct ccgaattcta gaggatccgg gtgacagccc
6841 tccgacgggt gacagccctc cgacgggtga cagccctccg aattcgagct cggtacccgg
6901 ggatctgtcg acctcgatcg agatcttcgc aagacccttc ctctatataa ggaagttcat
6961 ttcatttgga gaggacacgc tgaagctagt cgactctagc ctcgagacca tgggaaatat
7021 atgtgtcggc ggatccagga tggcccatca ggtgaactcc ccagaccgag ttagtaacaa
7081 ctcgggtgac gaagataacg taacgtccag tcaactgctg agcgtcagac atcaacttgc
7141 ggagtctgct ggtgtaccaa gagatcagca tgaatttgtt agtaaccaag cacctcaaag
7201 cctgagaaat cgctacaaca atctttactc acatacgcaa agaacactgg atatggcgga
7261 catgcagcat aggtacatga cgggagcgtc aggaatcaat ccgggaatgc tgccacatga
7321 gaatgtggac gatatgcgta gcgctataac tgattggagt gacatgcgcg aagctctgca
7381 gtacgcaatg ggtatccatg ccgacatccc accgtctcca gagcgatttg ttgcgactat
7441 gaacccgaac ggatcaattc gaatgtcaac actttctcct agcccgtacc gtaactggca
7501 atgaactagt cgatccaggc ctcccagctt tcgtccgtat catcggtttc gacaacgttc
7561 gtcaagttca atgcatcagt ttcattgccc acacaccaga atcctactaa gtttgagtat
7621 tatggcattg gaaaagctgt tttcttctat catttgttct gcttgtaatt tactgtgttc
7681 tttcagtttt tgttttcgga catcaaaatg caaatggatg gataagagtt aataaatgat
7741 atggtccttt tgttcattct caaattatta ttatctgttg tttttacttt aatgggttga
7801 atttaagtaa gaaaggaact aacagtgtga tattaaggtg caatgttaga catataaaac
7861 agtctttcac ctctctttgg ttatgtcttg aattggtttg tttcttcact tatctgtgta
7921 atcaagttta ctatgagtct atgatcaagt aattatgcaa tcaagttaag tacagtatag
7981 gctttttgtg tcgaaggggt accgagtcga gaattcactg gccgtcgttt tacaacgtcg
8041 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
8101 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
8161 gaatggcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
8221 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
8281 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
8341 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
8401 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
8461 tttcggaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
8521 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
8581 gaaaagaaaa accaccccag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
8641 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
8701 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
8761 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
8821 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
8881 gtaacgatga cagagcgttg ctgcctgtga tcaaatatca tctccctcgc agagatccga
8941 attatcagcc ttcttattca tttctcgctt aaccgtgaca ggctgtcgat cttgagaact
9001 atgccgacat aataggaaat cgctggataa agccgctgag gaagctgagt ggcgctattt
9061 ctttagaagt gaacgttgac gatatcaact cccctatcca ttgctcaccg aatggtacag
9121 gtcggggacc cgaagttccg actgtcggcc tgatgcatcc ccggctgatc gaccccagat
9181 ctggggctga gaaagcccag taaggaaaca actgtaggtt cgagtcgcga gatcccccgg
9241 aaccaaagga agtaggttaa acccgctccg atcaggccga gccacgccag gccgagaaca
9301 ttggttcctg taggcatcgg gattggcgga tcaaacacta aagctactgg aacgagcaga
9361 agtcctccgg ccgccagttg ccaggcggta aaggtgagca gaggcacggg aggttgccac
9421 ttgcgggtca gcacggttcc gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg
9481 ccgacaggat ctagcgctgc gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg
9541 caaatagccc ccaggaccgc catcaatcgt atcgggctac ctagcagagc ggcagagatg
9601 aacacgacca tcagcggctg cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg
9661 tagaccgaaa taaacaacaa gctccagaat agcgaaatat taagtgcgcc gaggatgaag
9721 atgcgcatcc accagattcc cgttggaatc tgtcggacga tcatcacgag caataaaccc
9781 gccggcaacg cccgcagcag cataccggcg acccctcggc ctcgctgttc gggctccacg
9841 aaaacgccgg acagatgcgc cttgtgagcg tccttggggc cgtcctcctg tttgaagacc
9901 gacagcccaa tgatctcgcc gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt
9961 tcagcgaacg cctccatggg ctttttctcc tcgtgctcgt aaacggaccc gaacatctct
10021 ggagctttct tcagggccga caatcggatc tcgcggaaat cctgcacgtc ggccgctcca
10081 agccgtcgaa tctgagcctt aatcacaatt gtcaatttta atcctctgtt tatcggcagt
10141 tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc
10201 ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc
10261 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
10321 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
10381 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
10441 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
10501 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
10561 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
10621 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
10681 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
10741 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
10801 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
10861 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
10921 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
10981 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
11041 tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc
11101 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
11161 ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg
11221 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
11281 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
11341 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
11401 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
11461 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
11521 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
11581 tcttgccctg cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct
11641 gagagccaaa acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt
11701 tgcgccacat ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
11761 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
11821 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
11881 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
11941 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
12001 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
12061 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
12121 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
12181 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
12241 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
12301 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
12361 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
12421 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
12481 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
12541 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
12601 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
12661 ataacatagt atcgacggag ccgattttga aaccacaatt atgggtgatg ctgccaactt
12721 actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtg tctatcagct
12781 gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc
12841 agcgctatct ctgctctcac tgccgtaaaa catggcaact gcagttcact tacaccgctt
12901 ctcaacccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg
12961 caacagcccg cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag
13021 gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat
13081 ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta
13141 tgacagtctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct
13201 ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg
13261 gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca
13321 gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc
13381 gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat
13441 aaaacactat caataagttg gagtcattac ccaattatga tagaatttac aagctataag
13501 gttattgtcc tgggtttcaa gcattagtcc atgcaagttt ttatgctttg cccattctat
13561 agatatattg ataagcgcgc tgcctatgcc ttgccccctg aaatccttac atacggcgat
13621 atcttctata taaaagatat attatcttat cagtattgtc aatatattca aggcaatctg
13681 cctcctcatc ctcttcatcc tcttcgtctt ggtagctttt taaatatggc gcttcataga
13741 gtaattctgt aaaggtccaa ttctcgtttt catacctcgg tataatctta cctatcacct
13801 caaatggttc gctgggttta tcgcaccccc gaacacgagc acggcacccg cgaccactat
13861 gccaagaatg cccaaggtaa aaattgccgg ccccgccatg aagtccgtga atgccccgac
13921 ggccgaagtg aagggcaggc cgccacccag gccgccgccc tcactgcccg gcacctggtc
13981 gctgaatgtc gatgccagca cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct
14041 gatcgcccat cccgttactg ccccgatccc ggcaatggca aggactgcca gcgctgccat
14101 ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc
14161 gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg
14221 cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt
14281 aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc
14341 tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc
14401 cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat
14461 accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc
14521 aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct
14581 gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc
14641 ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccgcggt
14701 gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca
14761 gcccagcggc gagggcaacc agcccgg
//
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