| 质粒名称: | pSR58.6 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 63176 |
| 存储实验室: | Jeffrey Tabor |
|
载体骨架基本信息 |
|
| 载体名称: | pProTet.E333 |
| 载体出品公司: | Clontech |
| 载体抗性: | 氯霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 3936 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，合成生物学 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CcaR |
| 物种来源: | Synechocystis sp. 6803 |
| 插入大小: | 705 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSR58.6 was a gift from Jeffrey Tabor (Addgene plasmid # 63176 ; http://n2t.net/addgene:63176 ; RRID:Addgene_63176) |
| 参考文献部分: | Refactoring and optimization of light-switchable escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. 10.1021/sb500273n PubMed 25250630 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSR58.6 3936 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses CcaR constitutively and sfGFP under the PcpcG2-172
promoter.
ACCESSION .
VERSION .
KEYWORDS pSR58.6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3936)
AUTHORS Schmidl SR, Sheth RU, Wu A, Tabor JJ
TITLE Refactoring and optimization of light-switchable escherichia coli
two-component systems.
JOURNAL ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n.
Epub 2014 Oct 11.
PUBMED 25250630
REFERENCE 2 (bases 1 to 3936)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3936
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 795..866
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 882..909
/label=T7Te terminator
/note="phage T7 early transcription terminator"
RBS 1205..1216
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1223..1936
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
terminator 1963..2034
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2050..2077
/label=T7Te terminator
/note="phage T7 early transcription terminator"
rep_origin complement(2279..2867)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2359..2378)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 2955..3049
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(3070..3729)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 3644..3663
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(3730..3832)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind 3898..3916
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ttgacggcta gctcagtcct aggtacagtg ctagctgtta aacgctcctc gtaagaacac
61 gggaaagcaa tgagaattct tttagtggag gatgatttgc cgctggcgga aacccttgct
121 gaagcattga gtgaccagct ttacaccgtt gatattgcca ccgacgcttc cctcgcctgg
181 gactatgcct cccgactgga atatgacctc gttattttgg atgtgatgct gccggagttg
241 gacgggatta ccctctgtca aaaatggcga tcgcacagtt atttaatgcc aattttgatg
301 atgacagcca gggatacgat caatgataaa atcacgggct tggatgcggg ggcggatgat
361 tatgtggtca agccagtgga tttgggggag ttatttgcca gggtgcgagc tttgttgcgt
421 cggggttgtg caacgtgcca accagtttta gagtgggggc caatcaggtt ggatccaagc
481 acctatgaag ttagttatga caatgaggtt ttgtctttga cccgcaagga atacagcatt
541 ctggaattac tactccgcaa tggccgtcgg gtgctaagtc ggagcatgat tatcgatagt
601 atctggaagt tggagagtcc cccagaggaa gatacggtta aggtgcatgt gcggagtttg
661 cgacaaaaat taaaaagtgc cggtttatca gcagatgcca ttgaaacggt ccatggcatt
721 gggtatcgtc tggccaattt aacggaaaaa tctttgtgcc aagggaaaaa ctagtaataa
781 tctagaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
841 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
901 ctttctgcgt ttatagtaaa tcactgcata attcgtgtcg ctcaaggcgc actcccgttc
961 tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg
1021 gttagctagt caagcccatt gtgcttttct ctatcaacct cagcttacct gaaggggtga
1081 acaggtctgg gttaattcat gttgcgaaat gtaacagttt tagtcgcatc agctaacttt
1141 ccgatttctt tacgattttc tccccctttt cttcaatttt actttgttag gatcgcattt
1201 ttaaaaagag gagaaatact agatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc
1261 tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg
1321 tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact
1381 gccggtacct tggccgactc tggtaacgac gctgacttat ggtgttcagt gctttgctcg
1441 ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg aaggctatgt
1501 gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa
1561 atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact ttaaagaaga
1621 cggcaatatc ctgggccata agctggaata caattttaac agccacaatg tttacatcac
1681 cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga
1741 tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt
1801 tctgctgcca gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga
1861 gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca cgcatggtat
1921 ggatgaactg tacaaatgat gataataatc tagaccaggc atcaaataaa acgaaaggct
1981 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
2041 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataggtaccc gtggatcctc
2101 tagagtcgac ctgcaggcat gcaagcttcc taggcgttcg gctgcggcga gcggtatcag
2161 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca tgcaagaaca
2221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
2281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
2341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
2401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
2461 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
2521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
2581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
2641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
2701 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
2761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
2821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
2881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
2941 tgactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
3001 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
3061 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
3121 tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag
3181 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
3241 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
3301 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
3361 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
3421 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
3481 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
3541 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
3601 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
3661 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
3721 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
3781 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
3841 ttttcgccag atatcgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
3901 aggcgtatca cgaggccctt tcgtcttcac ctcgag
//
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