LOCUS pT2-Venus 6123 bp ds-DNA circular SYN 29-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pT2VENUS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6123)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, August 29, 2016 from SnapGene Viewer 3.1.4
http://www.biofeng.com/
FEATURES Location/Qualifiers
source 1..6123
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(61..79)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(100..116)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 124..140
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(148..178)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 193..214
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(502..1090)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1261..2121)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2122..2226)
/gene="bla"
/note="AmpR promoter"
rep_origin 2253..2708
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 2849..2865
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2875..2893
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 3295..3416
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
intron 4169..5177
/note="chimeric intron"
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
promoter complement(5178..5453)
/note="chicken beta-actin promoter"
enhancer 5456..5759
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 gggatcctct agctagagtc gacctcgatc gagggggggc ccggtaccca gcttttgttc
61 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
121 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
181 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
241 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
301 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
361 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
421 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
481 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
541 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
601 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
661 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
721 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
781 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
841 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
901 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
961 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
1021 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
1081 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
1141 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
1201 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
1261 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
1321 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
1381 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
1441 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
1501 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
1561 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
1621 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
1681 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
1741 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
1801 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
1861 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
1921 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
1981 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
2041 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
2101 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
2161 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
2221 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
2281 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
2341 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
2401 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
2461 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
2521 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
2581 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
2641 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
2701 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
2761 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
2821 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
2881 gactcactat agggcgaatt ggagctcgga tccctataca gttgaagtcg gaagtttaca
2941 tacacttaag ttggagtcat taaaactcgt ttttcaacta ctccacaaat ttcttgttaa
3001 caaacaatag ttttggcaag tcagttagga catctacttt gtgcatgaca caagtcattt
3061 ttccaacaat tgtttacaga cagattattt cacttataat tcactgtatc acaattccag
3121 tgggtcagaa gtttacatac actaagttga ctgtgccttt aaacagcttg gaaaattcca
3181 gaaaatgatg tcatggcttt agaagctcta gtcgtgctag ctggacgcac tcgaggattt
3241 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttac gcgttaagat
3301 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
3361 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
3421 acggccgctc acttgtacag ctcgtccatg cccagggtga tgccggcggc ggtcacgaac
3481 tccagcagca ccatgtggtc cctcttctcg ttagggtcct tgctcagggc gctctggtag
3541 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
3601 tagtggtcgg ccagctgcac gccgccgtcc tcgatgttgt gcctgatctt gaagttggcc
3661 ttgatgccgt tcttctgctt gtcggcggtg atgtacacgt tgtggctgtt gtagttgtac
3721 tccagcttgt ggcccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatt
3781 ctgttcacca gggtgtcgcc ctcgaacttc acctcggctc tggtcttgta gttgccgtcg
3841 tccttgaaga agatggttct ctcctgcacg tagccctcag gcatggcgga cttgaagaag
3901 tcgtgctgct tcatgtggtc ggggtatctg gcgaagcact gcaggccgta gcccagggtg
3961 gtcaccaggg taggccaggg cacaggcagc ttgccggtgg tgcagatcag cttcagggtc
4021 agcttgccgt aggtggcgtc gccctcgccc tcgccgctca cggagaactt gtggccgttc
4081 acgtcgccgt ccagctccac caggatgggc accacgccgg tgaacagctc ctcgcccttg
4141 gacaccatgg tctagattaa ttaaggatct gtaggaaaaa gaagaaggca tgaacatggt
4201 tagcagaggc tctagagccg ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc
4261 ccccccgaag gcagccgtcc ccctgcggca gccccgaggc tggagatgga gaaggggacg
4321 gcggcgcggc gacgcacgaa ggccctcccc gcccatttcc ttcctgccgg cgccgcaccg
4381 cttcgcccgc gcccgctaga gggggtgcgg cggcgcctcc cagatttcgg ctccgccaga
4441 tttgggacaa aggaagtccc tgcgccctct cgcacgatta ccataaaagg caatggctgc
4501 ggctcgccgc gcctcgacag ccgccggcgc tccggggccg ccgcgcccct cccccgagcc
4561 ctccccggcc cgaggcggcc ccgccccgcc cggcaccccc acctgccgcc accccccgcc
4621 cggcacggcg agccccgcgc cacgccccgc acggagcccc gcacccgaag ccgggccgtg
4681 ctcagcaact cggggagggg ggtgcagggg ggggttacag cccgaccgcc gcgcccacac
4741 cccctgctca cccccccacg cacacacccc gcacgcagcc tttgttcccc tcgcagcccc
4801 cccgcaccgc ggggcaccgc ccccggccgc gctcccctcg cgcacacgcg gagcgcacaa
4861 agccccgcgc cgcgcccgca gcgctcacag ccgccgggca gcgcgggccg cacgcggcgc
4921 tccccacgca cacacacacg cacgcacccc ccgagccgct cccccccgca caaagggccc
4981 tcccggagcc ctttaaggct ttcacgcagc cacagaaaag aaacgagccg tcattaaacc
5041 aagcgctaat tacagcccgg aggagaaggg ccgtcccgcc cgctcacctg tgggagtaac
5101 gcggtcagtc agagccgggg cgggcggcgc gaggcggcgc ggagcggggc acggggcgaa
5161 ggcaacgcag cgactcccgc ccgccgcgcg cttcgctttt tatagggccg ccgccgccgc
5221 cgcctcgcca taaaaggaaa ctttcggagc gcgccgctct gattggctgc cgccgcacct
5281 ctccgcctcg ccccgccccg cccctcgccc cgccccgccc cgcctggcgc gcgccccccc
5341 cccccccgcc cccatcgctg cacaaaataa ttaaaaaata aataaataca aaattggggg
5401 tggggagggg ggggagatgg ggagagtgaa gcagaacgtg gggctcacct cgacccatgg
5461 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
5521 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
5581 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatag tccacccatt
5641 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
5701 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
5761 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
5821 agcttgtgga aggctactcg aaatgtttga cccaagttaa acaatttaaa ggcaatgcta
5881 ccaaatacta attgagtgta tgtaaacttc tgacccactg ggaatgtgat gaaagaaata
5941 aaagctgaaa tgaatcattc tctctactat tattctgata tttcacattc ttaaaataaa
6001 gtggtgatcc taactgacct aagacaggga atttttacta ggattaaatg tcaggaattg
6061 tgaaaaagtg agtttaaatg tatttggcta aggtgtatgt aaacttccga cttcaactgt
6121 ata
//
