| 质粒名称: | pEE491 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 149292 |
| 存储实验室: | Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pEE515 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 11610 bp |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos |
| 物种来源: | 烟草脆裂病毒 |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | 35S |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AarI (破坏) |
| 3’克隆位点: | AarI (破坏) |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pEE491 was a gift from Daniel Voytas (Addgene plasmid # 149292 ; http://n2t.net/addgene:149292 ; RRID:Addgene_149292) |
| 参考文献部分: | Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs. Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas DF. Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 10.1038/s41477-020-0670-y. 10.1038/s41477-020-0670-y PubMed 32483329 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEE491 11610 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented
gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2.
ACCESSION .
VERSION .
KEYWORDS pEE491
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11610)
AUTHORS Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
DF
TITLE Multiplexed heritable gene editing using RNA viruses and mobile
single guide RNAs.
JOURNAL Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi:
10.1038/s41477-020-0670-y.
PUBMED 32483329
REFERENCE 2 (bases 1 to 11610)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11610
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 427..772
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 715..735
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
misc_RNA 2327..2402
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_RNA 2610..2685
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_RNA 2893..2968
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
CDS 3540..3557
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 3592..3844
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 3866..3890
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 5191..5820
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 6249..7322
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 7388..7582
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(7818..7840)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 7926..8066
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 7981..8000
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(8081..8098)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8252..8840)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8332..8351)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10265..11059)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 11484..11508
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
ORIGIN
1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
2101 ttgagtttcg gagcatcttg ttctggggtt tcacactatc tttagagaaa gtgttaagtt
2161 aattaagtta tcttaattaa gagcataatt atactgattt gtctctcgtt gatagagtct
2221 atcattctgt tactaaaaat ttgacaactc ggtttgctga cctactggtt actgtatcac
2281 ttacccgagt taacgagtct agaaacgtgt gaaagaaaca attgaggttt tagagctaga
2341 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
2401 gctccctagt ctataaatat aagagaccct cttatagtaa gcagagttgt tggagacgtt
2461 cttgatccgt ttaatagatc aatcactcta aaggttactt atggccaaaa caaagcacca
2521 gtggtctagt ggtagaatag taccctgcca cggtacagac ccgggttcga ttcccggctg
2581 gtgcagtgct tggtagtagc gactccatgg ttttagagct agaaatagca agttaaaata
2641 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgctccct agtctataaa
2701 tataagagac cctcttatag taagcagagt tgttggagac gttcttgatc cgtttaatag
2761 atcaatcact ctaaaggtta cttatggcca aaacaaagca ccagtggtct agtggtagaa
2821 tagtaccctg ccacggtaca gacccgggtt cgattcccgg ctggtgcaca ggttgattgt
2881 cttctcaagc aggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
2941 acttgaaaaa gtggcaccga gtcggtgctc cctagtctat aaatataaga gaccctctta
3001 tagtaagcag agttgttgga gacgttcttg atccgtttaa tagatcaatc actctaaagg
3061 ttacttatgg ccaaagtgcc gggcatgtcc cgaagacatt aaactacggt tctttaagta
3121 gatccgtgtc tgaagtttta ggttcaattt aaacctacga gattgacatt ctcgactgat
3181 cttgattgat cggtaagtct tttgtaattt aattttcttt ttgattttat tttaaattgt
3241 tatctgtttc tgtgtataga ctgtttgaga tcggcgtttg gccgactcat tgtcttacca
3301 taggggaacg gactttgttt gtgttgttat tttatttgta ttttattaaa attctcaacg
3361 atctgaaaaa gcctcgcggc taagagattg ttggggggtg agtaagtact tttaaagtga
3421 tgatggttac aaaggcaaaa ggggtaaaac ccctcgccta cgtaagcgtt attacgcccg
3481 tctgtactta tatcagtaca ctgacgagtc cctaaaggac gaaacgggag aacgctagcc
3541 accaccacca ccaccacgtg tgaattacag gtgaccagct cgaatttccc cgatcgttca
3601 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
3661 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
3721 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
3781 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
3841 gatcgggaat taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
3901 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
3961 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
4021 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg
4081 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
4141 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
4201 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
4261 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
4321 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
4381 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
4441 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
4501 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
4561 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
4621 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
4681 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
4741 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
4801 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
4861 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
4921 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
4981 gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
5041 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
5101 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
5161 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
5221 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
5281 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
5341 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
5401 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
5461 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
5521 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
5581 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
5641 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
5701 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
5761 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
5821 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
5881 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
5941 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
6001 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
6061 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
6121 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
6181 ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag
6241 gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
6301 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
6361 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
6421 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
6481 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
6541 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
6601 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
6661 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
6721 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
6781 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
6841 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
6901 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
6961 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
7021 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
7081 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
7141 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
7201 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
7261 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
7321 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc
7381 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
7441 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
7501 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
7561 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
7621 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
7681 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
7741 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
7801 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
7861 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
7921 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
7981 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
8041 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
8101 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
8161 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
8221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
8281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
8341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
8401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
8461 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
8521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
8581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
8641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
8701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
8761 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
8821 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
8881 ggaacgaaaa ctcacgttaa gggctgatga atcccctaat gatttttatc aaaatcatta
8941 agttaaggta gatacacatc ttgtcatatg atcaaatggt ttcgccaaaa atcaataatc
9001 agacaacaaa atgtgcgaac tcgatatttt acacgactct ctttaccaat tctgccccga
9061 attacactta aaacgactca acagcttaac gttggcttgc cacgccttac ttgactgtaa
9121 aactctcact cttaccgaac ttggccgtaa cctgccaacc aaagcgagaa caaaacataa
9181 catcaaacga atcgaccgat tgttaggtaa tcgtcacctc cacaaagagc gactcgctgt
9241 ataccgttgg catgctagct ttatctgttc gggcaatacg atgcccattg tacttgttga
9301 ctggtctgat atccgtgagc aaaaacggct tatggtattg cgagcttcag tcgcactaca
9361 cggtcgttct gttactcttt atgagaaagc gttcccgctt tcagagcaat gttcaaagaa
9421 agctcatgac caatttctag ccgaccttgc gagcattcta ccgagtaaca ccacaccgct
9481 cattgtcagt gatgctggct ttaaagtgcc atggtataaa tccgttgaga agctgggttg
9541 gtactggtta agtcgagtaa gaggaaaagt acaatatgca gacctaggag cggaaaactg
9601 gaaacctatc agcaacttac atgatatgtc atctagtcac tcaaagactt taggctataa
9661 gaggctgact aaaagcaatc caatctcatg ccaaattcta ttgtataaat ctcgctctaa
9721 aggccgaaaa aatcagcgct cgacacggac tcattgtcac cacccgtcac ctaaaatcta
9781 ctcagcgtcg gcaaaggagc catggattct agcaactaac ttacctgttg aaattcgaac
9841 acccaaacaa cttgttaata tctattcgaa gcgaatgcag attgaagaaa ccttccgaga
9901 cttgaaaagt cctgcctacg gactaggcct acgccatagc cgaacgagca gctcagagcg
9961 ttttgatatc atgctgctaa tcgccctgat gcttcaacta acatgttggc ttgcgggcgt
10021 tcatgctcag aaacaaggtt gggacaagca cttccaggct aacacagtca gaaatcgaaa
10081 cgtactctca acagttcgct taggcatgga agttttgcgg cattctggct acacaataac
10141 aagggaagac tcactcgtgg ctgcaaccct gcttactcaa aatctattca cacatggtta
10201 cgttttgggg aaattatgag gggatctctc agcgttaagg gattttggtc atgcattcta
10261 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
10321 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
10381 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
10441 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
10501 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
10561 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
10621 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
10681 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
10741 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
10801 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
10861 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
10921 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
10981 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
11041 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
11101 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
11161 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
11221 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
11281 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
11341 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
11401 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
11461 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
11521 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
11581 cctaggccac catgttgggc ccggcgcgcc
//
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