MAP2-GCaMP3

LOCUS       MAP2-GCaMP3      10997 bp DNA     circular SYN 25-AUG-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10997)
  AUTHORS   Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD
  TITLE     Efficient conversion of astrocytes to functional midbrain 
            dopaminergic neurons using a single polycistronic vector.
  JOURNAL   PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub
            2011 Dec 9.
  PUBMED    22174877
REFERENCE   2  (bases 1 to 10997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2024 from SnapGene Server 7.0.3
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28719. doi"; pages: " 
            10.1371/journal.pone.0028719. Epub 2011 Dec 9"
FEATURES             Location/Qualifiers
     source          1..10997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3856..3875
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3856..3874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3906..3930
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3931..5283
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPVGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             3931..5283
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPVGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      4027..4036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4317..4338
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4444..4465)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4705..4724)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(5284..5308)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5334..5375
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5452..6040
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5505..5525)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5923..5934)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6043..6059)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6044..6060)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6565..6745
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6771..6788)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6777..7001
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7047..7475
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7134..7153)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7344..7365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7484..7504)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7489..7818
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7669..7804
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7731..7750
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7866..7913
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7932..8306
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8436..8557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8473..8492)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8527..8546
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8606..8622)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8606..8622)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8619..8641)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8630..8646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8654..8684)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8699..8720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8837..8854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9008..9596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9088..9107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9767..10627)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10390..10409
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10628..10732)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10807..10826)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaacagctg gcctttttgg ttctcatcga attttgctcc
     2401 attcactggt tcaggttgct aacaatgctg taaaaattaa cttatttatt gacatttttc
     2461 tccttccctt ttccccagga aataaatgca ggaaattatt ccacacttaa cctccctaca
     2521 caagggcact gtaaaatagg gatattcaac tgctaatttc agttgctaac atggagggga
     2581 tacagattat gccaaagatt gaattctctg cctgggccct cagggactga aaagccccag
     2641 gaccaagctg gttgccacct ccttttgctt ttgtatgtaa atagattgcc cccagaaatc
     2701 acatttctaa ttctctgttt tttttttctt tttctcttgt aaggaaaaca tggccaagtg
     2761 cataattata actgttaaaa gggtattgaa ttttctgtag gaaaatatac ataaatatat
     2821 gcatttcttt aatgaagata tttattttag aatataaata agcaatatgc gatagttaaa
     2881 aatattgtaa ttatctgcca atgacaaaac atcaacaaat ctctgatgtc cttaatgcag
     2941 ataataaaaa ttcccataaa ctagtattta gtcatgtcca gtttgtgtag cttaacttgt
     3001 ttgtaatcct atatgcctga aaactgtttc tctatttaag cggtgtgtgt gtgtgcgtgc
     3061 gtgcgtgcgt gtgtgtgtgt gtgtgtctgg cattaacaca aaggagacca gatgatgaca
     3121 ttcataggct gatagactcg actcctgggg acacacttgg atggaggcaa ttaaaatgcc
     3181 tgtcaggcat ttggtaccag aagcccctat ggcaaaccgc taaatcgtaa gtgagggctg
     3241 catcctattg tcagtgaatg atggcggagc tgagtggctt gtcatcattc tcgaaaattc
     3301 tgagactagt gagctctctc cctctctccc actcgcctta ttttccagtt cgccactgcg
     3361 gggacttcta atttgcagct tccctttccc cggcacacac agacacgagc tggtggcttg
     3421 cagactgcgt ctgtctggag ctagagagcc agagagccag cctgtgggga taatgctccc
     3481 ggagaaggat tctgcagcag ttctcaaagg ctagacttga gtggtattgc tgcatatgcg
     3541 ctggtaatgg tttggggctt ttaattttat ttcgtttcat tttcaattcg tttttcatag
     3601 gatgtgcttt atgtaatctc agagtaaaga tcttttaaaa ttcattattg agcttcctaa
     3661 atattcaagt actgcatttt ttttgtgcta cagacctcca ggcatagatt tattatttga
     3721 gtataaagaa tgcatttgct tactggtgag ttataatttt tgatatttag ttaacaaatc
     3781 catatatata tatatatata tgtttctttt gttttgtttc ggtagattct tcagcttgtc
     3841 tctaaccgac tcgagtaata cgactcacta tagggagacc caagctggct aggtaagctt
     3901 gatcaacaag tttgtacaaa aaagcaggct atgggttctc atcatcatca tcatcatggt
     3961 atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
     4021 gatctcgcca ccatggtcga ctcatcacgt cgtaagtgga ataagacagg tcacgcagtc
     4081 agagctatag gtcggctgag ctcactcgag aacgtctata tcaaggccga caagcagaag
     4141 aacggcatca aggcgaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
     4201 gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     4261 cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg
     4321 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     4381 ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     4441 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt
     4501 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     4561 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     4621 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccgt aggctacatc
     4681 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4741 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4801 ggcaacatcc tggggcacaa gctggagtac aacacgcgtg accaactgac tgaagagcag
     4861 atcgcagaat ttaaagaggc tttctcccta tttgacaagg acggggatgg gacaataaca
     4921 accaaggagc tggggacggt gatgcggtct ctggggcaga accccacaga agcagagctg
     4981 caggacatga tcaatgaagt ggatgccgac ggtgacggca caatcgactt ccctgagttc
     5041 ctgacaatga tggcaagaaa aatgaaagac acagacagtg aagaagaaat tagagaagcg
     5101 ttccgtgtgt ttgataagga tggcaatggc tacatcagtg cagcagagct tcgccacgtg
     5161 atgacaaacc ttggagagaa gttaacagat gaagaggttg atgaaatgat cagggaagca
     5221 gacatcgatg gggatggtca ggtaaactac gaagagtttg tacaaatgat gacagcgaag
     5281 taaacccagc tttcttgtac aaagtggttg atctagaggg cccgcggttc gaaggtaagc
     5341 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat cgccatcacc
     5401 attgagttta aacccgctga tcagcctcaa ttcgatatca agcttatcga taatcaacct
     5461 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5521 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5581 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5641 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5701 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5761 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5821 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5881 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5941 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6001 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     6061 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     6121 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     6181 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6241 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     6301 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     6361 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     6421 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     6481 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     6541 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     6601 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6661 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6721 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     6781 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6841 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6901 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6961 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     7021 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     7081 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7141 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7201 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7261 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7321 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7381 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7441 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     7501 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7561 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7621 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7681 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7741 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7801 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     7861 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     7921 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     7981 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     8041 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     8101 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     8161 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     8221 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     8281 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     8341 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     8401 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     8461 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8521 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     8581 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8641 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8701 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8761 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8821 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8881 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8941 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9001 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9061 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9121 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9181 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9241 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9301 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9361 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9421 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9481 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9541 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9601 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9661 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9721 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9781 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9841 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9901 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9961 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10021 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10081 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10141 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10201 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10261 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10321 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10381 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10441 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10501 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10561 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10621 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10681 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10741 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
    10801 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
    10861 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
    10921 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
    10981 ggccagatat acgcgtt
//