| 质粒名称: | PGKneoF2L2DTA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 13445 |
| 存储实验室: | Philippe Soriano |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2900 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达,Cre/Lox |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PGKneoF2L2DTA,PGKneobpA,PGKdtabpA |
| 物种来源: | -- |
| 插入大小: | 3600 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | See map(未破坏) |
| 3' 克隆位点: | See map (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | PGKneoF2L2DTA was a gift from Philippe Soriano (Addgene plasmid # 13445 ; http://n2t.net/addgene:13445 ; RRID:Addgene_13445) |
| 参考文献部分: | Context-specific requirements for Fgfr1 signaling through Frs2 and Frs3 during mouse development. Hoch RV, Soriano P. Development. 2006 Feb . 133(4):663-73. 10.1242/dev.02242 PubMed 16421190 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS PGKneoF2L2DTA 6461 bp ds-DNA circular SYN 12-NOV-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS PGKneoF2L2DTA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6461)
AUTHORS Hoch RV, Soriano P
TITLE Context-specific requirements for Fgfr1 signaling through Frs2 and
Frs3 during mouse development.
JOURNAL Development. 2006 Feb . 133(4):663-73.
PUBMED 16421190
REFERENCE 2 (bases 1 to 6461)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 12, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6461
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 4..459
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(91..110)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 301..322
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 585..607
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 599..616
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 626..645
/label=T7
/note="T7 promoter, forward primer"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 677..693
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 677..693
/label=pBluescriptSK
/note="For pBluescript vector"
protein_bind complement(707..740)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind complement(796..843)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
promoter 856..1355
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 1369..2172
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(1432..1451)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 2042..2061
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 2214..2438
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind complement(2475..2522)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
protein_bind complement(2561..2594)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
promoter 2673..3172
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 3199..3771
/codon_start=1
/gene="Corynebacterium diphtheriae tox"
/product="diphtheria toxin fragment A"
/label=DTA
/translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
NFETRGKRGQDAMYEYMAQACAGNRVRR"
intron 3848..3913
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
polyA_signal 3987..4211
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(4273..4293)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(4273..4291)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4312..4328)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4312..4328)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4325..4347)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4336..4352
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4360..4390)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4405..4426
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4543..4560)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4714..5302)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4794..4813)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5473..6333)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6096..6115
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6334..6438)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
661 ccgcggtggc ggccgctcta gaactagtgg atccggaacc cttaatataa cttcgtataa
721 tgtatgctat acgaagttat taggtccctc gacctgcagg aattcgagct cgcccgggga
781 tcctctagag tcaacgaagt tcctatactt tctagagaat aggaacttcg gaataggaac
841 ttcgttaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
901 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
961 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
1021 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
1081 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
1141 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
1201 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
1261 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
1321 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagccaatat gggatcggcc
1381 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
1441 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
1501 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
1561 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
1621 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
1681 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
1741 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
1801 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
1861 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
1921 gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
1981 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
2041 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
2101 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
2161 gagttcttct gaggggatca attctctagc tagagctcgc tgatcagcct cgactgtgcc
2221 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
2281 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
2341 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
2401 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
2461 ctggggctcg aaacgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact
2521 tcgttctaga gtcgaatcaa gctgatccgg aacccttaat ataacttcgt ataatgtatg
2581 ctatacgaag ttattaggtc cctcgacctg cagcccaagc tagcttatcg ataccgtcga
2641 cggtatcgat aagcttgata tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca
2701 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
2761 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
2821 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
2881 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
2941 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
3001 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
3061 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
3121 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
3181 gtcctcgcca tggatcctga tgatgttgtt gattcttcta aatcttttgt gatggaaaac
3241 ttttcttcgt accacgggac taaacctggt tatgtagatt ccattcaaaa aggtatacaa
3301 aagccaaaat ctggtacaca aggaaattat gacgatgatt ggaaagggtt ttatagtacc
3361 gacaataaat acgacgctgc gggatactct gtagataatg aaaacccgct ctctggaaaa
3421 gctggaggcg tggtcaaagt gacgtatcca ggactgacga aggttctcgc actaaaagtg
3481 gataatgccg aaactattaa gaaagagtta ggtttaagtc tcactgaacc gttgatggag
3541 caagtcggaa cggaagagtt tatcaaaagg ttcggtgatg gtgcttcgcg tgtagtgctc
3601 agccttccct tcgctgaggg gagttctagc gttgaatata ttaataactg ggaacaggcg
3661 aaagcgttaa gcgtagaact tgagattaat tttgaaaccc gtggaaaacg tggccaagat
3721 gcgatgtatg agtatatggc tcaagcctgt gcaggaaatc gtgtcaggcg atctctttgt
3781 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
3841 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
3901 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcaga
3961 tcctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
4021 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
4081 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
4141 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
4201 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgagggggg gcccggtacc
4261 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
4321 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
4381 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
4441 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
4501 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
4561 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
4621 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
4681 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
4741 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
4801 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
4861 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
4921 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
4981 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
5041 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
5101 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
5161 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
5221 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
5281 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
5341 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
5401 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
5461 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
5521 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
5581 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
5641 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
5701 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
5761 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
5821 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
5881 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
5941 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
6001 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
6061 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
6121 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
6181 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
6241 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
6301 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
6361 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
6421 acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
