出品公司: | Clontech |
---|---|
载体名称: | pGAD424 |
质粒类型: | 酵母单杂交系统 |
高拷贝/低拷贝: | -- |
克隆方法: | -- |
启动子: | ADH1 |
载体大小: | 6659 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | -- |
宿主细胞(系): | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
买家导航
pGAD424载体质粒基本信息
pGAD424质粒图谱载体图谱和pGAD424载体序列质粒序列多克隆位点信息
pGAD424质粒载体简介
pGAD424质粒序列载体序列
LOCUS pGAD424 6659 bp DNA SYN DEFINITION pGAD424 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6659 /organism="pGAD424" /mol_type="other DNA" promoter 10..406 /label="yADH1_promoter" misc_feature 422..829 /label="Gal4_AD" CDS 422..877 /label="ORF frame 2" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*" misc_feature 431..487 /label="SV40_NLS" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*" misc_feature 452..472 /label="SV40 NLS" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*" misc_feature 458..472 /label="NLS" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*" misc_feature 491..829 /label="Gal4_AD" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*" misc_feature 755..772 /label="Gal4_AD_primer" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*" terminator 1104..1427 /label="yADH2_terminator" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*" misc_feature complement(1120..1143) /label="pJG4_5_rev_primer" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*" terminator 1241..1432 /label="yADH1_terminator" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*" misc_feature complement(1296..1320) /label="pHybLex_rev_primer" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*" gene complement(1546..2640) /label="LEU2 (variant)" /gene="LEU2 (variant)" /translation="MDKAELIPEPPKKKRKVELGTAANFNQSGNIADSSLSFTFTNSS NGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYN AFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKEIEFPGIRRPAEIYES*" CDS complement(1546..2640) /label="ORF frame 3" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" promoter complement(3083..3101) /label="M13_reverse_primer" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" misc_feature complement(3100..3122) /label="M13_pUC_rev_primer" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" promoter complement(3136..3165) /label="lac_promoter" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" gene complement(4248..5108) /label="Ampicillin" /gene="Ampicillin" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" CDS complement(4248..5108) /label="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(5150..5178) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature complement(5337..5359) /label="pGEX_3_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 5495..6659 /label="2micron2_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc 61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga 121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac 181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct 241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga 301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa 421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg 481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt 541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc 601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag 661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc 721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata 781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattccc 841 ggggatccgt cgacctgcag agatctatga atcgtagata ctgaaaaacc ccgcaagttc 901 acttcaactg tgcatcgtgc accatctcaa tttctttcat ttatacatcg ttttgccttc 961 ttttatgtaa ctatactcct ctaagtttca atcttggcca tgtaacctct gatctataga 1021 attttttaaa tgactagaat taatgcccat cttttttttg gacctaaatt cttcatgaaa 1081 atatattacg agggcttatt cagaagcttt ggacttcttc gccagaggtt tggtcaagtc 1141 tccaatcaag gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg 1201 tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat 1261 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc 1321 ttaggtttta aaacgaaaat tcttgttctt gagtaactct ttcctgtagg tcaggttgct 1381 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcccgaaat 1441 tcccctaccc tatgaacata ttccattttg taatttcgtg tcgtttctat tatgaatttc 1501 atttataaag tttatgtaca aatatcataa aaaaagagaa tctttttaag caaggatttt 1561 cttaacttct tcggcgacag catcaccgac ttcggtggta ctgttggaac cacctaaatc 1621 accagttctg atacctgcat ccaaaacctt tttaactgca tcttcaatgg ccttaccttc 1681 ttcaggcaag ttcaatgaca atttcaacat cattgcagca gacaagatag tggcgatagg 1741 gtcaacctta ttctttggca aatctggagc agaaccgtgg catggttcgt acaaaccaaa 1801 tgcggtgttc ttgtctggca aagaggccaa ggacgcagat ggcaacaaac ccaaggaacc 1861 tgggataacg gaggcttcat cggagatgat atcaccaaac atgttgctgg tgattataat 1921 accatttagg tgggttgggt tcttaactag gatcatggcg gcagaatcaa tcaattgatg 1981 ttgaaccttc aatgtaggaa attcgttctt gatggtttcc tccacagttt ttctccataa 2041 tcttgaagag gccaaaacat tagctttatc caaggaccaa ataggcaatg gtggctcatg 2101 ttgtagggcc atgaaagcgg ccattcttgt gattctttgc acttctggaa cggtgtattg 2161 ttcactatcc caagcgacac catcaccatc gtcttccttt ctcttaccaa agtaaatacc 2221 tcccactaat tctctgacaa caacgaagtc agtaccttta gcaaattgtg gcttgattgg 2281 agataagtct aaaagagagt cggatgcaaa gttacatggt cttaagttgg cgtacaattg 2341 aagttcttta cggattttta gtaaaccttg ttcaggtcta acactacctg taccccattt 2401 aggaccaccc acagcaccta acaaaacggc atcaaccttc ttggaggctt ccagcgcctc 2461 atctggaagt gggacacctg tagcatcgat agcagcacca ccaattaaat gattttcgaa 2521 atcgaacttg acattggaac gaacatcaga aatagcttta agaaccttaa tggcttcggc 2581 tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg atcttcttag gggcagacat 2641 tagaatggta tatccttgaa atatatatat atattgctga aatgtaaaag gtaagaaaag 2701 ttagaaagta agacgattgc taaccaccta ttggaaaaaa caataggtcc ttaaataata 2761 ttgtcaactt caagtattgt gatgcaagca tttagtcatg aacgcttctc tattctatat 2821 gaaaagccgg ttccggcctc tcacctttcc tttttctccc aatttttcag ttgaaaaagg 2881 tatatgcgtc aggcgacctc tgaaattaac aaaaaatttc cagtcatcga atttgattct 2941 gtgcgatagc gcccctgtgt gttctcgtta tgttgaggaa aaaaataatg gttgctaaga 3001 gattcgaact cttgcatctt acgatacctg agtattccca cagttgggga tctcgactct 3061 agctagagga tcaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 3121 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 3181 tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 3241 ctgtcgtgcc agctggatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 3301 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 3361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 3421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 3481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 3541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 3601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 3661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 3721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 3781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 3841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 3901 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 3961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 4021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 4081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 4141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 4201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 4261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 4321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 4381 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 4441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 4501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 4561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 4621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 4681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 4741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 4801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 4861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 4921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 4981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 5041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 5101 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 5161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 5221 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 5281 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc 5341 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 5401 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg 5461 gcatcagagc agattgtact gagagtgcac cataacgcat ttaagcataa acacgcacta 5521 tgccgttctt ctcatgtata tatatataca ggcaacacgc agatataggt gcgacgtgaa 5581 cagtgagctg tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg 5641 ctttgaagtt cctattccga agttcctatt ctctagctag aaagtatagg aacttcagag 5701 cgcttttgaa aaccaaaagc gctctgaaga cgcactttca aaaaaccaaa aacgcaccgg 5761 actgtaacga gctactaaaa tattgcgaat accgcttcca caaacattgc tcaaaagtat 5821 ctctttgcta tatatctctg tgctatatcc ctatataacc tacccatcca cctttcgctc 5881 cttgaacttg catctaaact cgacctctac attttttatg tttatctcta gtattactct 5941 ttagacaaaa aaattgtagt aagaactatt catagagtga atcgaaaaca atacgaaaat 6001 gtaaacattt cctatacgta gtatatagag acaaaataga agaaaccgtt cataattttc 6061 tgaccaatga agaatcatca acgctatcac tttctgttca caaagtatgc gcaatccaca 6121 tcggtataga atataatcgg ggatgccttt atcttgaaaa aatgcacccg cagcttcgct 6181 agtaatcagt aaacgcggga agtggagtca ggcttttttt atggaagaga aaatagacac 6241 caaagtagcc ttcttctaac cttaacggac ctacagtgca aaaagttatc aagagactgc 6301 attatagagc gcacaaagga gaaaaaaagt aatctaagat gctttgttag aaaaatagcg 6361 ctctcgggat gcatttttgt agaacaaaaa agaagtatag attctttgtt ggtaaaatag 6421 cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg tttgaaaaat 6481 tagcgctctc gcgttgcatt tttgttttac aaaaatgaag cacagattct tcgttggtaa 6541 aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga 6601 aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat gaagcacaga tgcttcgtt //