| 出品公司: | -- |
|---|---|
| 载体名称: | pBE980a |
| 质粒类型: | 枯草杆菌 |
| 高拷贝/低拷贝: | -- |
| 复制子: | -- |
| 启动子: | P43 |
| 载体大小: | repB |
| 5’测序引物及序列: | -- |
| 3’测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5 alpha |
| 宿主细胞（系）: | -- |
| 备注: | 蛋白表达,分泌表达 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pBE980a载体质粒基本信息
pBE980a质粒图谱载体图谱和pBE980a载体序列质粒序列多克隆位点信息
pBE980a质粒载体简介
pBE980a质粒序列载体序列
LOCUS pbe980a 4562 bp DNA circular SYN 23-JUL-2013
DEFINITION bla gene was cutted from pBE980.
ACCESSION pBE980a
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4562)
AUTHORS Self
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 4562)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 3, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished."
COMMENT SECID/File created by SciEd Central, Scientific
COMMENT VNTDATE|783561600|
COMMENT VNTDBDATE|668304000|
COMMENT VNTNAME|pBE980a|
FEATURES Location/Qualifiers
CDS 93..179
/codon_start=1
/gene="sig pep"
/label=sig pep
CDS 573..1577
/codon_start=1
/gene="B. rep"
/product="replication protein"
/label=B. rep
CDS 1737..2507
/codon_start=1
/gene="kanR"
/label=kanR
CDS 2730..3128
/codon_start=1
/gene="bleoR"
/product="bleomycin resistance protein"
/label=bleoR
misc_feature complement(3525..4197)
/gene="E. ori"
/label=E. ori
misc_feature join(4506..4562,1..36)
/gene="p43 promoter"
/label=p43 promoter
ORIGIN
1 cgcgattatg taaaatataa agtgatagcg gtaccaggag ggctggaaga agcagaccgc
61 taacacagta cataaaaaag gagacatgaa cgatgaacat caaaaagttt gcaaaacaag
121 caacagtatt aacctttact accgcactgc tggcaggagg cgcaactcaa gcttttgcct
181 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc tagcttcagc
241 acaattccaa gaaaaacacg atttagaacc taaaaagaac gaatttgaac taactcataa
301 ccgagaggta aaaaaagaac gaagtcgaga tcagggaatg agtttataaa ataaaaaaag
361 cacctgaaaa ggtgtctttt tttgatggtt ttgaacttgt tctttcttat cttgatacat
421 atagaaataa cgtcattttt attttagttg ctgaaaggtg cgttgaagtg ttggtatgta
481 tgtgttttaa agtattgaaa acccttaaaa ttggttgcac agaaaaaccc catctgttaa
541 agttataagt gactaaacaa ataactaaat agatgggggt ttcttttaat attatgtgtc
601 ctaatagtag catttattca gatgaaaaat caagggtttt agtggacaag acaaaaagtg
661 gaaaagtgag accatggaga gaaaagaaaa tcgctaatgt tgattacttt gaacttctgc
721 atattcttga atttaaaaag gctgaaagag taaaagattg tgctgaaata ttagagtata
781 aacaaaatcg tgaaacaggc gaaagaaagt tgtatcgagt gtggttttgt aaatccaggc
841 tttgtccaat gtgcaactgg aggagagcaa tgaaacatgg cattcagtca caaaaggttg
901 ttgctgaagt tattaaacaa aagccaacag ttcgttggtt gtttctcaca ttaacagtta
961 aaaatgttta tgatggcgaa gaattaaata agagtttgtc agatatggct caaggatttc
1021 gccgaatgat gcaatataaa aaaattaata aaaatcttgt tggttttatg cgtgcaacgg
1081 aagtgacaat aaataataaa gataattctt ataatcagca catgcatgta ttggtatgtg
1141 tggaaccaac ttattttaag aatacagaaa actacgtgaa tcaaaaacaa tggattcaat
1201 tttggaaaaa ggcaatgaaa ttagactatg atccaaatgt aaaagttcaa atgattcgac
1261 cgaaaaataa atataaatcg gatatacaat cggcaattga cgaaactgca aaatatcctg
1321 taaaggatac ggattttatg accgatgatg aagaaaagaa tttgaaacgt ttgtctgatt
1381 tggaggaagg tttacaccgt aaaaggttaa tctcctatgg tggtttgtta aaagaaatac
1441 ataaaaaatt aaaccttgat gacacagaag aaggcgattt gattcataca gatgatgacg
1501 aaaaagccga tgaagatgga ttttctatta ttgcaatgtg gaattgggaa cggaaaaatt
1561 attttattaa agagtagttc aacaaacggg ccagtttgtt gaagattaga tgctataatt
1621 gttattaaaa ggattgaagg atgcttagga agacgagtta ttaatagctg aataagaacg
1681 gtgctctcca aatattctta tttagaaaag caaatctaaa attatctgaa aagggaatga
1741 gaatagtgaa tggaccaata ataatgacta gagaagaaag aatgaagatt gttcatgaaa
1801 ttaaggaacg aatattggat aaatatgggg atgatgttaa ggctattggt gtttatggct
1861 ctcttggtcg tcagactgat gggccctatt cggatattga gatgatgtgt gtcatgtcaa
1921 cagaggaagc agagttcagc catgaatgga caaccggtga gtggaaggtg gaagtgaatt
1981 ttgatagcga agagattcta ctagattatg catctcaggt ggaatcagat tggccgctta
2041 cacatggtca atttttctct attttgccga tttatgattc aggtggatac ttagagaaag
2101 tgtatcaaac tgctaaatcg gtagaagccc aaacgttcca cgatgcgatt tgtgccctta
2161 tcgtagaaga gctgtttgaa tatgcaggca aatggcgtaa tattcgtgtg caaggaccga
2221 caacatttct accatccttg actgtacagg tagcaatggc aggtgccatg ttgattggtc
2281 tgcatcatcg catctgttat acgacgagcg cttcggtctt aactgaagca gttaagcaat
2341 cagatcttcc ttcaggttat gaccatctgt gccagttcgt aatgtctggt caactttccg
2401 actctgagaa acttctggaa tcgctagaga atttctggaa tgggattcag gagtggacag
2461 aacgacacgg atatatagtg gatgtgtcaa aacgcatacc attttgaacg atgacctcta
2521 ataattgtta atcatgttgg ttacgtattt attaacttct cctagtatta gtaattatca
2581 tggctgtcat ggcgcattaa cggaataaag ggtgtgctta aatcgggcca ttttgcgtaa
2641 taagaaaaag gattaattat gagcgaattg aattaataat aaggtaatag atttacatta
2701 gaaaatgaaa ggggatttta tgcgtgagaa tgttacagtc tatcccggca ttgccagtcg
2761 gggatattaa aaagagtata ggtttttatt gcgataaact aggtttcact ttggttcacc
2821 atgaagatgg attcgcagtt ctaatgtgta atgaggttcg gattcatcta tgggaggcaa
2881 gtgatgaagg ctggcgctct cgtagtaatg attcaccggt ttgtacaggt gcggagtcgt
2941 ttattgctgg tactgctagt tgccgcattg aagtagaggg aattgatgaa ttatatcaac
3001 atattaagcc tttgggcatt ttgcacccca atacatcatt aaaagatcag tggtgggatg
3061 aacgagactt tgcagtaatt gatcccgaca acaatttgat tagctttttt caacaaataa
3121 aaagctaaaa tctattatta atctgttcag caatcgggcg cgattgctga ataaaagata
3181 cgagagacct ctcttgtatc ttttttattt tgagtggttt tgtccgttac actagaaaac
3241 cgaaagacaa taaaaatttt attcttgctg agtctggctt tcggtaagct agacaaaacg
3301 gacaaaataa aaattggcaa gggtttaaag gtggagattt tttgagtgat cttctcaaaa
3361 aatactacct gtcccttgct gatttttaaa cgagcacgag agcaaaaccc ccctttgctg
3421 aggtggcaga gggcaggttt ttttgtttct tttttctcgt aaaaaaaaga aaggtcttaa
3481 aggttttatg gttttggtcg gcactgaatt ctgaagctat ggtgtgtgag caaaaggcca
3541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
3601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
4021 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
4081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
4141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
4201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
4261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
4321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
4381 ctgtctattt cgtcaaacat caccctcttg ctaaagcggc caaggacgct gccgccgggg
4441 ctgtttgcgt ttttgccgtg atttcgtgta tcattggttt acttattttt ttgccaaagc
4501 tgtaatggct gaaaattctt acatttattt tacattttta gaaatgggcg tgaaaaaaag
4561 cg
//
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