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pTK-Green-Renilla-Luc载体质粒基本信息
pTK-Green-Renilla-Luc质粒图谱载体图谱和pTK-Green-Renilla-Luc载体序列质粒序列多克隆位点信息
pTK-Green-Renilla-Luc质粒载体简介
pTK-Green Renilla Luc 载体说明
The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.
pTK-Green Renilla Luc 载体特征:
Weak (TK) constitutive promoter for co-transfection and normalization
Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
High-copy pUC bacterial DNA replication origin
pTK-Green-Renilla-Luc质粒序列载体序列
LOCUS Exported 6004 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Control vector for constitutive low-level expression of
intracellular green Renilla luciferase under control of the HSV TK
promoter.
ACCESSION .
VERSION .
KEYWORDS pTK-Green Renilla Luc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6004)
AUTHORS Thermo Scientific (Pierce)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6004
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
promoter 26..777
/note="HSV TK promoter"
/note="herpes simplex virus thymidine kinase promoter"
CDS 790..1725
/codon_start=1
/product="green-emitting variant of Renilla luciferase"
/note="Green Renilla Luciferase"
/note="human codon-optimized"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNATSSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGSALAFHYAYEHQDRIKAIVHMESVVDVIESWMGWPDIEEEL
ALIKSEEGEKMVLENNFFVETLLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKLFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFLQEDAPDEMGKYIKSFVERVLKNEQ"
polyA_signal 1748..1859
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
promoter 1982..2311
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2162..2297
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 2359..2406
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 2425..3024
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 3154..3275
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS complement(3328..4188)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4452..5040
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5782..5798
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
misc_feature 5913..6004
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 actagtctcg aggaattcca agcttaatga gtcttcggac ctcgcggggg ccgcttaagc
61 ggtggttagg gtttgtctga cgcgggggga gggggaagga acgaaacact ctcattcgga
121 ggcggctcgg ggtttggtct tggtggccac gggcacgcag aagagcgccg cgatcctctt
181 aagcaccccc ccgccctccg tggaggcggg ggtttggtcg gcgggtggta actggcgggc
241 cgctgactcg ggcgggtcgc gcgccccaga gtgtgacctt ttcggtctgc tcgcagaccc
301 ccgggcggcg ccgccgcggc ggcgacgggc tcgctgggtc ctaggctcca tggggaccgt
361 atacgtggac aggctctgga gcatccgcac gactgcggtg atattaccgg agaccttctg
421 cgggacgagc cgggtcacgc ggctgacgcg gagcgtccgt tgggcgacaa acaccaggac
481 ggggcacagg tacactatct tgtcacccgg aggcgcgagg gactgcagga gcttcaggga
541 gtggcgcagc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga
601 agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc
661 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca
721 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaagga
781 tccgccacca tggccagcaa ggtgtacgac cccgagcagc ggaagcggat gatcaccggc
841 cctcagtggt gggctcggtg caagcagatg aacgtgctgg acagcttcat caactactac
901 gacagcgaga agcacgccga gaacgccgtg atcttcctgc acggcaacgc caccagcagc
961 tacctgtggc ggcacgtggt gccccacatc gagcctgtgg ccagatgcat catccccgac
1021 ctgatcggca tgggcaagag cggcaagtcc ggcaacggca gctaccggct gctggaccac
1081 tacaagtacc tgaccgcttg gtttgagctg ctgaacctgc ccaagaagat catcttcgtc
1141 ggccacgact ggggcagcgc cctggccttt cactacgcct acgagcacca ggaccggatc
1201 aaggccatcg tgcacatgga aagcgtggtg gacgtgatcg agagctggat gggctggccc
1261 gacatcgagg aagaactggc cctgatcaag agcgaagagg gcgagaagat ggtgctggaa
1321 aacaacttct tcgtggaaac cctgctgccc agcaagatca tgcggaagct ggaacccgaa
1381 gagttcgccg cctacctgga acccttcaaa gaaaagggcg aagtgcggag gcccaccctg
1441 agctggccca gagagatccc cctggtcaag ggcggcaagc ccgacgtggt gcagatcgtg
1501 cggaactaca acgcctacct gcgggccagc gacgacctgc ctaagctgtt catcgagagc
1561 gaccccggct tcttcagcaa cgccatcgtg gaaggcgcca agaagttccc caacaccgag
1621 ttcgtgaaag tgaagggcct gcacttcctc caggaagatg cccccgacga gatgggcaag
1681 tacatcaaga gcttcgtgga acgggtgctg aagaacgagc agtgagcggc cgcaaaatca
1741 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
1801 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatca
1861 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
1921 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
1981 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
2041 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
2101 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
2161 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
2221 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
2281 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
2341 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
2401 tacgacaagg tgaggaacta aaccatgacc gagtacaagc ccacggtgcg cctcgccacc
2461 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
2521 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
2581 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
2641 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
2701 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
2761 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
2821 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
2881 cgcgccgggg tgcccgcctt cctggagaca tccgcgcccc gcaacctccc cttctacgag
2941 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
3001 atgacccgca agcccggtgc ctgacacgtg ctacgagatt tcgattccac cgccgccttc
3061 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
3121 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
3181 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
3241 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
3301 agctagagct tggcgtaatc atggtcatta ccaatgctta atcagtgagg cacctatctc
3361 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
3421 gatacgggag ggcttaccat ctggccccag cgctgcgatg ataccgcgag aaccacgctc
3481 accggctccg gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
3541 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
3601 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc gctacaggca tcgtggtgtc
3661 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
3721 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
3781 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
3841 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
3901 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
3961 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
4021 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
4081 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
4141 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatat tcttcctttt
4201 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
4261 tatttagaaa aataaacaaa taggggtcag tgttacaacc aattaaccaa ttctgaacat
4321 tatcgcgagc ccatttatac ctgaatatgg ctcataacac cccttgctca tgaccaaaat
4381 cccttaacgt gagttacgcg cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
4441 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
4501 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
4561 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
4621 gcccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
4681 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
4741 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
4801 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
4861 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
4921 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
4981 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
5041 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
5101 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
5161 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
5221 ggcgagagta gggaactgcc aggcatcaaa ctaagcagaa ggcccctgac ggatggcctt
5281 tttgcgtttc tacaaactct ttctgtgttg taaaacgacg gccagtctta agctcgggcc
5341 ccctgggcgg ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc
5401 ggcgggtttt tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaagggcg
5461 cctgtcactt tgcttgatat atgagaatta tttaacctta taaatgagaa aaaagcaacg
5521 cactttaaat aagatacgtt gctttttcga ttgatgaaca cctataatta aactattcat
5581 ctattattta tgattttttg tatatacaat atttctagtt tgttaaagag aattaagaaa
5641 ataaatctcg aaaataataa agggaaaatc agtttttgat atcaaaatta tacatgtcaa
5701 cgataataca aaatataata caaactataa gatgttatca gtatttatta tcatttagaa
5761 taaattttgt gtcgccctta attgtgagcg gataacaatt acgagcttca tgcacagtgg
5821 cgttgacatt gattattgac tagcatgttc tttcctgcgt tatcccctga ttctgtggat
5881 aaccgtatta ccgccatgca ttagttatta ataacatacg ctctccatca aaacaaaacg
5941 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
6001 ctct
//
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