| 质粒名称: | pHH21-BNA-Luc |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 187924 |
| 存储实验室: | Arindam Mondal |
|
载体骨架基本信息 |
|
| 载体名称: | pHH21 |
| 载体出品公司: | Clontech |
| 空载体大小: | 2828bp |
| 完整质粒大小: | 4637bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | B-NA-Luc、Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus) |
| 插入大小: | 1809bp |
| 启动子: | RNA polymease I Promoter |
| 克隆方法: | 未知 |
| 5’克隆位点: | AgeI(未破坏) |
| 5’测序引物 | AAAACGCTGGGCGTTAATCAAAGAGGCG |
| 3’测序引物 | GGGGGACACTTTCGGACATCTGGTC |
|
文献引用方法 |
|
| 材料和方法部分: | pHH21-BNA-Luc was a gift from Arindam Mondal (Addgene plasmid # 187924 ; http://n2t.net/addgene:187924 ; RRID:Addgene_187924) |
| 参考文献部分: | A Comprehensive Roadmap Towards the Generation of an Influenza B Reporter Assay Using a Single DNA Polymerase-Based Cloning of the Reporter RNA Construct. Kedia N, Banerjee S, Mondal A. Front Microbiol. 2022 May 25;13:868367. doi: 10.3389/fmicb.2022.868367. eCollection 2022. 10.3389/fmicb.2022.868367 PubMed 35694292 |
买家导航
pHH21-BNA-Luc载体质粒基本信息
pHH21-BNA-Luc质粒图谱载体图谱和pHH21-BNA-Luc载体序列质粒序列多克隆位点信息
pHH21-BNA-Luc质粒载体简介
pHH21-BNA-Luc质粒序列载体序列
LOCUS pHH21-BNA-Luc 4631 bp DNA circular SYN 25-AUG-2024
DEFINITION Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs
of NA segment of Influenza B/Brisbane/60/2008 virus by RNA
polymerase I in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4631)
AUTHORS Kedia N, Banerjee S, Mondal A
TITLE A Comprehensive Roadmap Towards the Generation of an Influenza B
Reporter Assay Using a Single DNA Polymerase-Based Cloning of the
Reporter RNA Construct.
JOURNAL Front Microbiol. 2022 May 25;13:868367. doi:
10.3389/fmicb.2022.868367. eCollection 2022.
PUBMED 35694292
REFERENCE 2 (bases 1 to 4631)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Front
Microbiol."; date: "2022-05-25"; volume: "13:868367. doi"; pages: "
10.3389/fmicb.2022.868367. eCollection 2022"
FEATURES Location/Qualifiers
source 1..4631
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(517..2169)
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind 2085..2103
/label=LucNrev
/note="Firefly luciferase, reverse primer"
primer_bind complement(2431..2448)
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
terminator 2601..2687
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2779..2806
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 2825..2916
/gene="bla"
/label=AmpR promoter
CDS 2917..3777
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3135..3154)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3948..4536
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4437..4456
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gatcacgatc ctttctggcg agtccccgtg cggagtcgga gagcgctccc tgagcgcgcg
61 tgcggcccga gaggtcgcgc ctggccggcc ttcggtccct cgtgtgtccc ggtcgtagga
121 ggggccggcc gaaaatgctt ccggctcccg ctctggagac acgggccggc cccctgcgtg
181 tggcacgggc ggccgggagg gcgtccccgg cccggcgctg ctcccgcgtg tgtcctgggg
241 ttgaccagag ggccccgggc gctccgtgtg tggctgcgat ggtggcgttt ttggggacag
301 gtgtccgtgt cgcgcgtcgc ctgggccggc ggcgtggtcg gtgacgcgac ctcccggccc
361 cgggggaggt atatctttcg ctccgagtcg gcattttggg ccgccgggtt attagtagta
421 acaagagcat ttttcagaaa caattaagtt tagtaaggac aattgttcaa acaaaacagg
481 aacaaagggt ttagaacaga ctcaaccatt cctccattac acggcgatct ttccgccctt
541 cttggccttt atgaggatct ctctgatttt tcttgcgtcg agttttccgg taagaccttt
601 cggtacttcg tccacaaaca caactcctcc gcgcaacttt ttcgcggttg ttacttgact
661 ggcgacgtaa tccacgatct ctttttccgt catcgtcttt ccgtgctcca aaacaacaac
721 ggcggcggga agttcaccgg cgtcatcgtc gggaagacct gcgacacctg cgtcgaagat
781 gttggggtgt tggagcaaga tggattccaa ttcagcggga gccacctgat agcctttgta
841 cttaatcaga gacttcaggc ggtcaacgat gaagaagtgt tcgtcttcgt cccagtaagc
901 tatgtctcca gaatgtagcc atccatcctt gtcaatcaag gcgttggtcg cttccggatt
961 gtttacataa ccggacataa tcataggacc tctcacacac agttcgcctc tttgattaac
1021 gcccagcgtt ttcccggtat ccagatccac aaccttcgct tcaaaaaatg gaacaacttt
1081 accgaccgcg cccggtttat catccccctc gggtgtaatc agaatagctg atgtagtctc
1141 agtgagccca tatccttgcc tgatacctgg cagatggaac ctcttggcaa ccgcttcccc
1201 gacttcctta gagaggggag cgccaccaga agcaatttcg tgtaaattag ataaatcgta
1261 tttgtcaatc agagtgcttt tggcgaagaa ggagaatagg gttggcacca gcagcgcact
1321 ttgaatcttg taatcctgaa ggctcctcag aaacagctct tcttcaaatc tatacattaa
1381 gacgactcga aatccacata tcaaatatcc gagtgtagta aacattccaa aaccgtgatg
1441 gaatggaaca acacttaaaa tcgcagtatc cggaatgatt tgattgccaa aaataggatc
1501 tctggcatgc gagaatctca cgcaggcagt tctatgaggc agagcgacac ctttaggcag
1561 accagtagat ccagaggagt tcatgatcag tgcaattgtc ttgtccctat cgaaggactc
1621 tggcacaaaa tcgtattcat taaaaccggg aggtagatga gatgtgacga acgtgtacat
1681 cgactgaaat ccctggtaat ccgttttaga atccatgata ataatttttt ggatgattgg
1741 gagctttttt tgcacgttca aaattttttg caaccccttt ttggaaacga acaccacggt
1801 aggctgcgaa atgcccatac tgttgagcaa ttcacgttca ttataaatgt cgttcgcggg
1861 cgcaactgca actccgataa ataacgcgcc caacaccggc ataaagaatt gaagagagtt
1921 ttcactgcat acgacgattc tgtgatttgt attcagccca tatcgtttca tagcttctgc
1981 caaccgaacg gacatttcga agtactcagc gtaagtgatg tccacctcga tatgtgcatc
2041 tgtaaaagca attgttccag gaaccagggc gtatctcttc atagccttat gcagttgctc
2101 tccagcggtt ccatcttcca gcggatagaa tggcgccggg cctttcttta tgtttttggc
2161 gtcttccatt gttcattttt ggcctatttg cttcagtttt gagaagatgc tctgcttctg
2221 ctccccccca acttcggagg tcgaccagta ctccgggcga cactttgttt ttttttttcc
2281 cccgatgctg gaggtcgacc agatgtccga aagtgtcccc cccccccccc cccggcgcgg
2341 agcggcgggg ccactctgga ctcttttttt tttttttttg gggatcggcc gctagcttgg
2401 ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag
2461 cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat
2521 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
2581 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
2641 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
2701 atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
2761 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
2821 ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
2881 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
2941 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
3001 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
3061 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
3121 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
3181 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
3241 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
3301 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
3361 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
3421 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
3481 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
3541 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
3601 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
3661 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
3721 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
3781 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
3841 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
3901 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
3961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
4021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
4081 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
4141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
4201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
4261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
4321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
4381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
4441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
4501 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
4561 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
4621 tcattaatgc a
//
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