| 质粒名称: | BE4-Gam |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 100806 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 空载体大小: | 3402 bp |
| 完整质粒大小: | 9444 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | BE4-Gam |
| 物种来源: | R. norvegicus (rat), Synthetic; S. pyogenes |
| 插入大小: | 6042bp |
| 启动子: | CMV |
| 克隆方法: | Gibson Cloning |
| 5’克隆位点: | AgeI(未破坏) |
| 5’测序引物 | T7 |
| 3’测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: |
BE4-Gam was a gift from David Liu (Addgene plasmid # 100806 ; http://n2t.net/addgene:100806 ; RRID:Addgene_100806)
|
| 参考文献部分: | Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, Kim YB, Badran AH, Liu DR. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. aao4774 [pii] PubMed 28875174 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS BE4-Gam 9444 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses BE4-Gam in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS BE4-Gam
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9444)
AUTHORS Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW,
Kim YB, Badran AH, Liu DR
TITLE Improved base excision repair inhibition and bacteriophage Mu Gam
protein yields C:G-to-T:A base editors with higher efficiency and
product purity.
JOURNAL Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774.
eCollection 2017 Aug.
PUBMED 28875174
REFERENCE 2 (bases 1 to 9444)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9444
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 286..306
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 377..396
/label=T7
/note="T7 promoter, forward primer"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 403..412
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 979..1662
/codon_start=1
/product="cytidine deaminase (C to U editing enzyme) from
rat"
/label=APOBEC-1
/note="can use ssDNA as a substrate (Komor et al., 2016)"
/translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
WATGLK"
CDS 1759..5859
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5890..6138
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 6169..6417
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 6430..6450
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6459..6476
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(6499..6516)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6505..6729
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6800..6816)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6800..6816)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6813..6835)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6824..6840
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6848..6878)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6893..6914
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7031..7048)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7202..7790)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7282..7301)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7961..8821)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8584..8603
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8822..8926)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9005..9024)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 9196..131
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggctaaacca
421 gcaaaacgta tcaagagtgc cgcagcggct tatgtgccac aaaaccgcga tgcggtgatt
481 accgatatta aacgcatcgg ggatttacag cgcgaagcat cacgtctgga aacggaaatg
541 aatgatgcca tcgcggaaat tacggagaaa tttgcggccc ggattgcacc gattaaaacc
601 gatattgaaa ccctttcaaa aggcgttcag ggatggtgtg aagcgaaccg cgacgaactg
661 acgaacggcg gcaaagtgaa gacggcgaat cttgtcaccg gtgatgtatc gtggcgggtc
721 cgtccaccat cagtaagtat tcgtggtatg gatgcagtga tggaaacgct ggagcgtctt
781 ggcctgcaac gctttattcg cacgaagcag gaaatcaaca aggaagcgat tttactggaa
841 ccgaaagcgg tcgcaggcgt tgccggaatt acagttaaat caggcattga ggatttttct
901 attattccat ttgaacagga agccggtatt agcggcagcg agactcccgg gacctcagag
961 tccgctacac ccgaaagtag ctcagagact ggcccagtgg ctgtggaccc cacattgaga
1021 cggcggatcg agccccatga gtttgaggta ttcttcgatc cgagagagct ccgcaaggag
1081 acctgcctgc tttacgaaat taattggggg ggccggcact ccatttggcg acatacatca
1141 cagaacacta acaagcacgt cgaagtcaac ttcatcgaga agttcacgac agaaagatat
1201 ttctgtccga acacaaggtg cagcattacc tggtttctca gctggagccc atgcggcgaa
1261 tgtagtaggg ccatcactga attcctgtca aggtatcccc acgtcactct gtttatttac
1321 atcgcaaggc tgtaccacca cgctgacccc cgcaatcgac aaggcctgcg ggatttgatc
1381 tcttcaggtg tgactatcca aattatgact gagcaggagt caggatactg ctggagaaac
1441 tttgtgaatt atagcccgag taatgaagcc cactggccta ggtatcccca tctgtgggta
1501 cgactgtacg ttcttgaact gtactgcatc atactgggcc tgcctccttg tctcaacatt
1561 ctgagaagga agcagccaca gctgacattc tttaccatcg ctcttcagtc ttgtcattac
1621 cagcgactgc ccccacacat tctctgggcc accgggttga aatctggtgg ttcttctggt
1681 ggttctagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga aagttctggt
1741 ggttcttctg gtggttctga taaaaagtat tctattggtt tagccatcgg cactaattcc
1801 gttggatggg ctgtcataac cgatgaatac aaagtacctt caaagaaatt taaggtgttg
1861 gggaacacag accgtcattc gattaaaaag aatcttatcg gtgccctcct attcgatagt
1921 ggcgaaacgg cagaggcgac tcgcctgaaa cgaaccgctc ggagaaggta tacacgtcgc
1981 aagaaccgaa tatgttactt acaagaaatt tttagcaatg agatggccaa agttgacgat
2041 tctttctttc accgtttgga agagtccttc cttgtcgaag aggacaagaa acatgaacgg
2101 caccccatct ttggaaacat agtagatgag gtggcatatc atgaaaagta cccaacgatt
2161 tatcacctca gaaaaaagct agttgactca actgataaag cggacctgag gttaatctac
2221 ttggctcttg cccatatgat aaagttccgt gggcactttc tcattgaggg tgatctaaat
2281 ccggacaact cggatgtcga caaactgttc atccagttag tacaaaccta taatcagttg
2341 tttgaagaga accctataaa tgcaagtggc gtggatgcga aggctattct tagcgcccgc
2401 ctctctaaat cccgacggct agaaaacctg atcgcacaat tacccggaga gaagaaaaat
2461 gggttgttcg gtaaccttat agcgctctca ctaggcctga caccaaattt taagtcgaac
2521 ttcgacttag ctgaagatgc caaattgcag cttagtaagg acacgtacga tgacgatctc
2581 gacaatctac tggcacaaat tggagatcag tatgcggact tatttttggc tgccaaaaac
2641 cttagcgatg caatcctcct atctgacata ctgagagtta atactgagat taccaaggcg
2701 ccgttatccg cttcaatgat caaaaggtac gatgaacatc accaagactt gacacttctc
2761 aaggccctag tccgtcagca actgcctgag aaatataagg aaatattctt tgatcagtcg
2821 aaaaacgggt acgcaggtta tattgacggc ggagcgagtc aagaggaatt ctacaagttt
2881 atcaaaccca tattagagaa gatggatggg acggaagagt tgcttgtaaa actcaatcgc
2941 gaagatctac tgcgaaagca gcggactttc gacaacggta gcattccaca tcaaatccac
3001 ttaggcgaat tgcatgctat acttagaagg caggaggatt tttatccgtt cctcaaagac
3061 aatcgtgaaa agattgagaa aatcctaacc tttcgcatac cttactatgt gggacccctg
3121 gcccgaggga actctcggtt cgcatggatg acaagaaagt ccgaagaaac gattactcca
3181 tggaattttg aggaagttgt cgataaaggt gcgtcagctc aatcgttcat cgagaggatg
3241 accaactttg acaagaattt accgaacgaa aaagtattgc ctaagcacag tttactttac
3301 gagtatttca cagtgtacaa tgaactcacg aaagttaagt atgtcactga gggcatgcgt
3361 aaacccgcct ttctaagcgg agaacagaag aaagcaatag tagatctgtt attcaagacc
3421 aaccgcaaag tgacagttaa gcaattgaaa gaggactact ttaagaaaat tgaatgcttc
3481 gattctgtcg agatctccgg ggtagaagat cgatttaatg cgtcacttgg tacgtatcat
3541 gacctcctaa agataattaa agataaggac ttcctggata acgaagagaa tgaagatatc
3601 ttagaagata tagtgttgac tcttaccctc tttgaagatc gggaaatgat tgaggaaaga
3661 ctaaaaacat acgctcacct gttcgacgat aaggttatga aacagttaaa gaggcgtcgc
3721 tatacgggct ggggacgatt gtcgcggaaa cttatcaacg ggataagaga caagcaaagt
3781 ggtaaaacta ttctcgattt tctaaagagc gacggcttcg ccaataggaa ctttatgcag
3841 ctgatccatg atgactcttt aaccttcaaa gaggatatac aaaaggcaca ggtttccgga
3901 caaggggact cattgcacga acatattgcg aatcttgctg gttcgccagc catcaaaaag
3961 ggcatactcc agacagtcaa agtagtggat gagctagtta aggtcatggg acgtcacaaa
4021 ccggaaaaca ttgtaatcga gatggcacgc gaaaatcaaa cgactcagaa ggggcaaaaa
4081 aacagtcgag agcggatgaa gagaatagaa gagggtatta aagaactggg cagccagatc
4141 ttaaaggagc atcctgtgga aaatacccaa ttgcagaacg agaaacttta cctctattac
4201 ctacaaaatg gaagggacat gtatgttgat caggaactgg acataaaccg tttatctgat
4261 tacgacgtcg atcacattgt accccaatcc tttttgaagg acgattcaat cgacaataaa
4321 gtgcttacac gctcggataa gaaccgaggg aaaagtgaca atgttccaag cgaggaagtc
4381 gtaaagaaaa tgaagaacta ttggcggcag ctcctaaatg cgaaactgat aacgcaaaga
4441 aagttcgata acttaactaa agctgagagg ggtggcttgt ctgaacttga caaggccgga
4501 tttattaaac gtcagctcgt ggaaacccgc caaatcacaa agcatgttgc acagatacta
4561 gattcccgaa tgaatacgaa atacgacgag aacgataagc tgattcggga agtcaaagta
4621 atcactttaa agtcaaaatt ggtgtcggac ttcagaaagg attttcaatt ctataaagtt
4681 agggagataa ataactacca ccatgcgcac gacgcttatc ttaatgccgt cgtagggacc
4741 gcactcatta agaaataccc gaagctagaa agtgagtttg tgtatggtga ttacaaagtt
4801 tatgacgtcc gtaagatgat cgcgaaaagc gaacaggaga taggcaaggc tacagccaaa
4861 tacttctttt attctaacat tatgaatttc tttaagacgg aaatcactct ggcaaacgga
4921 gagatacgca aacgaccttt aattgaaacc aatggggaga caggtgaaat cgtatgggat
4981 aagggccggg acttcgcgac ggtgagaaaa gttttgtcca tgccccaagt caacatagta
5041 aagaaaactg aggtgcagac cggagggttt tcaaaggaat cgattcttcc aaaaaggaat
5101 agtgataagc tcatcgctcg taaaaaggac tgggacccga aaaagtacgg tggcttcgat
5161 agccctacag ttgcctattc tgtcctagta gtggcaaaag ttgagaaggg aaaatccaag
5221 aaactgaagt cagtcaaaga attattgggg ataacgatta tggagcgctc gtcttttgaa
5281 aagaacccca tcgacttcct tgaggcgaaa ggttacaagg aagtaaaaaa ggatctcata
5341 attaaactac caaagtatag tctgtttgag ttagaaaatg gccgaaaacg gatgttggct
5401 agcgccggag agcttcaaaa ggggaacgaa ctcgcactac cgtctaaata cgtgaatttc
5461 ctgtatttag cgtcccatta cgagaagttg aaaggttcac ctgaagataa cgaacagaag
5521 caactttttg ttgagcagca caaacattat ctcgacgaaa tcatagagca aatttcggaa
5581 ttcagtaaga gagtcatcct agctgatgcc aatctggaca aagtattaag cgcatacaac
5641 aagcacaggg ataaacccat acgtgagcag gcggaaaata ttatccattt gtttactctt
5701 accaacctcg gcgctccagc cgcattcaag tattttgaca caacgataga tcgcaaacga
5761 tacacttcta ccaaggaggt gctagacgcg acactgattc accaatccat cacgggatta
5821 tatgaaactc ggatagattt gtcacagctt gggggtgact ctggtggttc tggaggatct
5881 ggtggttcta ctaatctgtc agatattatt gaaaaggaga ccggtaagca actggttatc
5941 caggaatcca tcctcatgct cccagaggag gtggaagaag tcattgggaa caagccggaa
6001 agcgatatac tcgtgcacac cgcctacgac gagagcaccg acgagaatgt catgcttctg
6061 actagcgacg cccctgaata caagccttgg gctctggtca tacaggatag caacggtgag
6121 aacaagatta agatgctctc tggtggttct ggaggatctg gtggttctac taatctgtca
6181 gatattattg aaaaggagac cggtaagcaa ctggttatcc aggaatccat cctcatgctc
6241 ccagaggagg tggaagaagt cattgggaac aagccggaaa gcgatatact cgtgcacacc
6301 gcctacgacg agagcaccga cgagaatgtc atgcttctga ctagcgacgc ccctgaatac
6361 aagccttggg ctctggtcat acaggatagc aacggtgaga acaagattaa gatgctctct
6421 ggtggttctc ccaagaagaa gaggaaagtc taaccggtca tcatcaccat caccattgag
6481 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
6541 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
6601 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
6661 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
6721 gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg acctctagct
6781 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
6841 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga
6901 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
6961 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
7021 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
7081 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
7141 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
7201 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
7261 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
7321 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
7381 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
7441 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
7501 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
7561 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
7621 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
7681 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
7741 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
7801 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
7861 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
7921 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
7981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
8041 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
8101 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
8161 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
8221 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
8281 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
8341 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
8401 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
8461 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
8521 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
8581 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
8641 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
8701 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
8761 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
8821 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
8881 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
8941 aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtcgactc
9001 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
9061 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
9121 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
9181 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
9241 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
9301 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
9361 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
9421 acttggcagt acatcaagtg tatc
//
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