| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pMETalpha C, pMETα C, pMETalphaC, pMETαC |
| 质粒类型: | 毕赤酵母蛋白表达载体（methanoloica酵母） |
| 表达水平: | 高拷贝 |
| 诱导方法: | 甲醇诱导 |
| 启动子: | AUG1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8049 bp |
| 5' 测序引物及序列: | AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´; alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´ |
| 3' 测序引物及序列: | AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´ |
| 载体标签: | C-V5， C-His，N-alpha分泌因子 |
| 载体抗性: | 氨苄 |
| 筛选标记: | ADE2 |
| 备注: | 含有alpha factor酵母分泌信号肽，利用甲醇诱导蛋白在methanoloica酵母中分泌表达蛋白。 |
| 产品目录号: | K1780-01 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pMETαC载体 pMETalpha C质粒基本信息
pMETαC质粒图谱 pMETalpha C载体图谱和pMETαC载体序列 pMETalpha C质粒序列多克隆位点信息
pMETαC质粒 pMETalpha C载体简介
pMETαC质粒序列 pMETalpha C载体序列
LOCUS pMET\alpha\C 8049 bp DNA circular 1-AUG-2013
DEFINITION pMET alpha C.
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT http://www.biofeng.com/
COMMENT VNTNAME|pMET alpha C|
COMMENT VNTOAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
misc_feature 1106..1130
/vntifkey="21"
/label=AUG1_fwd_primer
misc_feature complement(1668..1689)
/vntifkey="21"
/label=AUG1_rev_primer
CDS 3091..3666
/vntifkey="4"
/label=ORF\frame\1
CDS 3672..4805
/vntifkey="4"
/label=ORF\frame\3
CDS 4969..5967
/vntifkey="4"
/label=ORF\frame\1
gene complement(6780..7640)
/gene="Ampicillin"
/vntifkey="60"
CDS complement(6780..7640)
/vntifkey="4"
/label=ORF\frame\2
promoter complement(7682..7710)
/vntifkey="30"
/label=AmpR_promoter
sig_peptide 1197..1463
/vntifkey="94"
/label=Alpha\factor\secretion\signal
BASE COUNT 2363 a 1741 c 1568 g 2377 t
ORIGIN
1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag
61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt
121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac
181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca
241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac
301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa
361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc
421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa
481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg
541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc
601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa
661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct
721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct
781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg
841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa
901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat
961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct
1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt
1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac
1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcaaaatga
1201 gatttccttc aatttttact gcagttttat tcgcagcatc ctccgcatta gctgctccag
1261 tcaacactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact
1321 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg
1381 ggttattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc
1441 tcgagaaaag agaggctgaa gcatcgatga attcacgtgg cccagccggc cgtggatcca
1501 cgcgtcgtcg acccgcggcg gccgccagct tactagtagg taagcctatc cctaaccctc
1561 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga tctagtatac
1621 aattctaggg ctgcctgttt ggatattttt ataatttttg agagtttgcc aactaatgtt
1681 tttctcttct atgatattta tcatgtagtt ggatatcttt ttttctttga aaagttttta
1741 tttagttctt tctctctttt ttataatgtt ttcagtttct ttcctttata ttgaaaatac
1801 gatgttcttt gtctttcttt tttaaatcct caattataat gtgtaccgtt tgatcaactt
1861 gtaaaattgt ttgtagtcct gtaatcttaa ttgacgattg gttgatagtt taccccagta
1921 tattgtgcgg ctgagatatt acccgattcg gaagattgat tttttcaaca gacataaacc
1981 cataggagat ttagtacagc gacatatgct aattgcctga taatgctcac taacattttc
2041 tccttttaat ctggtatatg catacaggta tgttgatatg gccttgttga tcaaagctac
2101 gaaagtgtag actatttccc aaacagcccc caaccaccac ctcacacgcg cacactgcga
2161 agtgaaagcg ctcccttatt ttcttctccg ctggttcaaa acaaaaaaga atctaatctg
2221 tagacacacg ccccgtaaaa cccaatcttc atcatcgtaa ttttcagtga cggacgatcg
2281 atcaaccata tagtttgtta gtgactgagc ttcagtagtt gacctcttga agaaataacg
2341 tcttgaacaa catactttat acctaccaac attcgccatc aacaacagtg tgacactgac
2401 ataactttac atccattcat cgacaaaaaa atgtctctag ttcggattca tgcttatggg
2461 ttagctattt cgcccaatgt gtccatctga cattactatt ttgcatttta atttaattag
2521 aacttgacta gcgcactacc agcatatcat ctcatttccg taaataccaa atgtattata
2581 tattgaaagc ttttgaccag gttattataa aagaaacttc atgctcgaaa aagatcattt
2641 cgaaaagttg cctagtttca tgaaatttta aagcagttta tataaatttt accttttgat
2701 gcggaattga ctttttcttg aataatacat aacttttctt aaaagaatca aagacagata
2761 aaatttaaga gatattaaat attagtgaga agccgagaat tttgtaacac caacataaca
2821 ctgacatctt taacaacttt taattatgat acatttctta cgtcatgatt gattattaca
2881 gctatgctga caaatgactc ttgttgcatg gctacgaacc gggtaatact aagtgattga
2941 ctcttgctga ccttttatta agaactaaat ggacaatatt atggagcatt tcatgtataa
3001 attggtgcgt aaaatcgttg gatctctctt ctaagtacat cctactataa caatcaagaa
3061 aaacaagaaa atcggacaaa acaatcaagt atggattcta gaacagttgg tatattagga
3121 gggggacaat tgggacgtat gattgttgag gcagcaaaca ggctcaacat taagacggta
3181 atactagatg ctgaaaattc tcctgccaaa caaataagca actccaatga ccacgttaat
3241 ggctcctttt ccaatcctct tgatatcgaa aaactagctg aaaaatgtga tgtgctaacg
3301 attgagattg agcatgttga tgttcctaca ctaaagaatc ttcaagtaaa acatcccaaa
3361 ttaaaaattt acccttctcc agaaacaatc agattgatac aagacaaata tattcaaaaa
3421 gagcatttaa tcaaaaatgg tatagcagtt acccaaagtg ttcctgtgga acaagccagt
3481 gagacgtccc tattgaatgt tggaagagat ttgggttttc cattcgtctt gaagtcgagg
3541 actttggcat acgatggaag aggtaacttc gttgtaaaga ataaggaaat gattccggaa
3601 gctttggaag tactgaagga tcgtcctttg tcgccgaaaa atgggcacca tttactaaag
3661 aattagcagt catgattgtg agatctgtta acggtttagt gttttcttac ccaattgtag
3721 agactatcca caaggacaat atttgtgact tatgttatgc gcctgctaga gttccggact
3781 ccgttcaact taaggcgaag ttgttggcag aaaatgcaat caaatctttt cccggttgtg
3841 gtatatttgg tgtggaaatg ttctatttag aaacagggga attgcttatt aacgaaattg
3901 ccccaaggcc tcacaactct ggacattata ccattgatgc ttgcgtcact tctcaatttg
3961 aagctcattt gagatcaata ttggatttgc caatgccaaa gaatttcaca tctttctcca
4021 ccattacaac gaacgccatt atgctaaatg ttcttggaga caaacataca aaagataaag
4081 agctagaaac ttgcgaaaga gcattggcga ctccaggttc ctcagtgtac ttatatggaa
4141 aagagtctag acctaacaga aaagtaggtc acataaatat tattgcctcc agtatggcgg
4201 aatgtgaaca aaggctgaac tacattacag gtagaactga tattccaatc aaaatctctg
4261 tcgctcaaaa gttggacttg gaagcaatgg tcaaaccatt ggttggaatc atcatgggat
4321 cagactctga cttgccggta atgtctgccg catgtgcggt tttaaaagat tttggcgttc
4381 catttgaagt gacaatagtc tctgctcata gaactccaca taggatgtca gcatatgcta
4441 tttccgcaag caagcgtgga attaaaacaa ttatcgctgg agctggtggg gctgctcact
4501 tgccaggtat ggtggctgca atgacaccac ttcctgtcat cggtgtgccc gtaaaaggtt
4561 cttgtctaga tggagtagat tctttacatt caattgtgca aatgcctaga ggtgttccag
4621 tagctaccgt cgctattaat aatagtacga acgctgcgct gttggctgtc agactgcttg
4681 gcgcttatga ttcaagttat acaacgaaaa tggaacagtt tttattaaag caagaagaag
4741 aagttcttgt caaagcacaa aagttagaaa ctgtcggtta cgaagcttat ctagaaaaca
4801 agtaatatat aagtttattg atatacttgt acagcaaata attataaaat gatataccta
4861 ttttttaggc tttgttatga ttacatcaaa tgtggacttc atacatagaa atcaacgctt
4921 acaggtgtcc ttttttaaga atttcataca taagatctac tagaatcaat gttaaacctg
4981 ttatcattag aacagccatc tccagaaggt tctatccaag tgaaaacttt ggtcttcaag
5041 ccaaagactg ctaaaacaca aaccccagtc ccaattattg ttgttgctga aacttctact
5101 tctactccgg gtggtgttat agccaaagct tcgggcacta aagagccaag attagcaaac
5161 gatgaattga ccaaccaatt ctttggttct cctgctaaag agttttctat tgccagctta
5221 tcaaacgaac acaaagacgg taaagtcaag atcattattg atgctgggat caaagatgat
5281 gactcgacta attacgttat ttccaacact gttgctaagt tagttttaac cggtaagcaa
5341 ttattaggtt tcttatccaa ctacgaacca accatcgttg attttactgc tgaagctgca
5401 accgctgccc caactccagt tgttaagaaa gaacctgctg catctggtaa gcttgatgat
5461 gccaaattaa ttggtgttac tgctgacaag gaaaaagatt tctccaactg gtactcccaa
5521 attctaatga agggtgaaat gttagactac tacgatgtct ctggttgtta cattatgaga
5581 ccagcttctt acacaatttg ggaaaagatt caatcttggt tcgattcgaa gattaaagaa
5641 gatggtgtta ctaatgctta tttcccaatg tttgtctcat ccaaggtttt agaaaaggaa
5701 aaggaccaca tcgatggttt cgctcctgaa gttgcttggg ttaccagagc tggtaaatct
5761 gaattagaag aaccaattgc tattagacca acttctgaaa ctgttatggg taccctgcag
5821 gggccggcct taattaaggc gcgcccttcc tcgctcactg actcgctgcg ctcggtcgtt
5881 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
5941 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
6001 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
6061 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6121 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6181 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
6241 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
6301 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
6361 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
6421 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
6481 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
6541 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
6601 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
6661 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
6721 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
6781 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
6841 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
6901 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
6961 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
7021 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
7081 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
7141 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
7201 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
7261 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
7321 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
7381 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
7441 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
7501 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
7561 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
7621 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
7681 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
7741 ccgcgcacat ttccccgaaa agtgccacct gtatgcggtg tgaaataccg cacagatgcg
7801 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
7861 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
7921 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
7981 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc aagggcgatg
8041 gcccactac
//
